A5100745762- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Pluripotent Stem Cells Research
- Microfluidic and Bio-sensing Technologies
- Modular Robots and Swarm Intelligence
- Image and Signal Denoising Methods
- Photoacoustic and Ultrasonic Imaging
- Neuroscience and Neural Engineering
- Tissue Engineering and Regenerative Medicine
- Leaf Properties and Growth Measurement
- Additive Manufacturing and 3D Printing Technologies
- Advanced optical system design
- CAR-T cell therapy research
- Ophthalmology and Visual Impairment Studies
- Muscle Physiology and Disorders
- Image Processing Techniques and Applications
- RNA Interference and Gene Delivery
- Corneal surgery and disorders
- Optical Coherence Tomography Applications
- Advanced biosensing and bioanalysis techniques
- Graphene and Nanomaterials Applications
- Optical Systems and Laser Technology
- Genomics and Chromatin Dynamics
University of California, Los Angeles
2023-2025
Zhejiang University
2025
UCLA Health
2023
Northeastern University
2013
We investigate how matrix stiffness regulates chromatin reorganization and cell reprogramming find that acts as a biphasic regulator of epigenetic state fibroblast-to-neuron conversion efficiency, maximized at an intermediate 20 kPa. ATAC sequencing analysis shows the same trend accessibility to neuronal genes these levels. Concurrently, we observe peak levels histone acetylation acetyltransferase (HAT) activity in nucleus on kPa matrices, inhibiting HAT abolishes effects. G-actin cofilin,...
Synthetic Notch (synNotch) receptors are genetically encoded, modular synthetic that enable mammalian cells to detect environmental signals and respond by activating user-prescribed transcriptional programs. Although some materials have been modified present synNotch ligands with coarse spatial control, applications in tissue engineering generally require extracellular matrix (ECM)-derived scaffolds and/or finer positioning of multiple ligands. Thus, we develop here a suite activate for...
Abstract The role of transcription factors and biomolecules in cell type conversion has been widely studied. Yet, it remains unclear whether how intracellular mechanotransduction through focal adhesions (FAs) the cytoskeleton regulates epigenetic state reprogramming. Here, is shown that cytoskeletal structures mechanical properties cells are modulated during early phase induced neuronal (iN) reprogramming, with an increase actin assembly by Ascl1 transgene. reduction tension or adhesion at...
The integration of intramuscular fat—or marbling—into cultured meat will be critical for texture, mouthfeel, flavor, and thus consumer appeal. However, culturing muscle tissue with marbling is challenging since myocytes adipocytes have different media scaffold requirements optimal growth differentiation. Here, we present an approach to engineer multicomponent using myogenic adipogenic microtissues. key innovation in our the engineering microtissues scaffolds customized physical properties;...
Optical coherence tomography (OCT) is a promising technology, which could be used in variety of imaging applications. However, OCT images are usually degraded by speckle noise. Speckle noise reduction particularly challenging because it difficult to separate the and information components pattern. In this study, novel technique, based on robust principal component analysis (RPCA), presented applied for first time. The proposed technique gives an optimal estimate image domain transformations...
Extracellular matrices of living tissues exhibit viscoelastic properties, yet how these properties regulate chromatin and the epigenome remains unclear. Here, we show that substrates induce changes in nuclear architecture epigenome, with more pronounced effects on softer surfaces. Fibroblasts display larger nuclei, lower compaction, differential expression distinct sets genes related to cytoskeleton function, compared those elastic Slow-relaxing reduce lamin A/C enhance remodeling. These...
The predecessor of China Optics was and Applied Digest, which founded in 1985. At that time, it the only retrieval journal field optics China. end 2008, Digest renamed
Abstract Cell reprogramming and manufacturing have broad applications in tissue regeneration disease treatment. However, many derived cell types lack unique surface markers for protein‐based sorting, making it difficult to isolate these cells from mixed populations. Additionally, there is a need identify of interest at the early stages expansion. To address this challenge, nucleic acid‐based gold nanorod (NAGNR) fluorescent biosensor was engineered detect mRNA expression intracellular...
<title>Abstract</title> Immunotherapy shows great promise for cancer treatment, but its effectiveness and durability remain limited. Thus, there is unmet needs more robust technology immune cell engineering. Here we develop a scalable microfluidic platform to fabricate synthetic viscoelastic activating cells (SynVACs) with programmable mechanical chemical properties. We demonstrate that the nature of SynVACs significantly impacts T functionality. Compared rigid or elastic microspheres,...