A5000444771- Herpesvirus Infections and Treatments
- Cytomegalovirus and herpesvirus research
- Aortic Disease and Treatment Approaches
- Animal Virus Infections Studies
- Aortic aneurysm repair treatments
- Cardiac Valve Diseases and Treatments
- Animal Disease Management and Epidemiology
- Immune Cell Function and Interaction
- Venous Thromboembolism Diagnosis and Management
- Toxoplasma gondii Research Studies
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Liver Disease Diagnosis and Treatment
- Cardiac, Anesthesia and Surgical Outcomes
- Cardiac Structural Anomalies and Repair
- Vector-borne infectious diseases
- Infective Endocarditis Diagnosis and Management
- Hip and Femur Fractures
- Coronary Artery Anomalies
- Cardiac Arrhythmias and Treatments
- NF-κB Signaling Pathways
- Inflammatory Bowel Disease
- Organ and Tissue Transplantation Research
- Medical Research and Treatments
Affiliated Hospital of North Sichuan Medical College
2011-2025
Sichuan University
2008-2025
North Sichuan Medical University
2025
West China Hospital of Sichuan University
2008-2024
Shanghai Institute of Measurement and Testing Technology
2023
The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture
2020-2022
Wuhan Third Hospital
2021
Tongren Hospital
2021
Ghent University
2015-2016
People's Liberation Army 401 Hospital
2014
Abstract Background Duck plague virus (DPV) is the causative agent of Plague (DP) that causes significant morbidity and mortality throughout duck-producing areas world. The diagnosis DP currently relies on use live or inactivated whole DPV virion as antigens in ELISA, but it too laborious expensive for routine application, still difficult to get purified with current technology. Results In this study, we describe expression purification a recombinant Thymidine Kinase (TK) protein which makes...
To evaluate the safety and efficacy of percutaneous closure (PC) using modified double-disc ventricular septal defect (VSD) occluders in patients with ruptured sinus Valsalva aneurysm (RSVA).Between 2005 2012, 25 were enrolled our study for attempted PC. There five (20%) RSVA combined VSD two (8%) aortic regurgitation (AR). The median age was 45 years (24-74 years). QP/QS 2.4±0.6 (1.6-4.0). diameter lesions 6 mm (4-8 mm) while size 8 (6-12 mm). Twenty-three (92%) underwent PC successfully....
Murine cytomegalovirus (MCMV) Smith strain is widely used in mouse models to study HCMV infections. Due high serial passages, MCMV has acquired genetic and biological changes. Therefore, a low passaged would be more relevant develop models. Here, the pathogenesis of an infection with was compared that Belgian isolate HaNa1 BALB/c adult mice following oronasal inoculation either (104 TCID50/mouse) or (106 dose. Both strains were mainly replicating nasal mucosa submandibular glands for one two...
At present, alphaherpesviruses gI gene and its encoding protein have been extensively studied. It is likely that homolog play similar roles in virions direct cell-to-cell spread of alphaherpesviruses. But, little known about the characteristics DEV gene. In this study, we expressed presented basic properties protein.The special 1221-bp fragment containing complete open reading frame(ORF) duck enteritis virus(DEV) was extracted from plasmid pMD18-T-gI, then cloned into prokaryotic expression...
Systemic immune-inflammation index (SII) is a novel biomarker that can predict poor outcomes in tumours, nervous system diseases and chronic heart failure. Here, we investigated the predictive value of SII on postoperative short-term prognosis valve (HVDs).This retrospective cohort study enrolled all consecutive patients with HVDs (aortic stenosis, aortic regurgitation, mitral stenosis regurgitation) who underwent surgery (valve replacement or repair) at Affiliated Hospital North Sichuan...
Abstract Knowledge of the intracellular location a protein can provide useful insights into its function. Bioinformatic studies have predicted that DEV pUL38 mainly targets cytoplasm and nucleus. In this study, we obtained anti-pUL38 polyclonal sera. These antibodies were functional in western blotting immunofluorescence DEV-infected duck embryo fibroblasts (DEFs). was expressed as 51-kDa from 8 h post-infection onward, initially showing diffuse distribution throughout cytoplasm, later...
Abstract Acute type A aortic dissection (a-TAAD) is a severe disease characterized by high mortality, which can be fatal in elderly patients. The objective of this study was to investigate the safety and efficacy two-stage II hybrid arch repair (HAR) patients with acute (a-TAAD). This single-center, retrospective involving 119 a-TAAD, including 82 males 37 females, aged 22–81 years old. Eighty-eight underwent total replacement (TAR) frozen elephant trunk (FET) implantation (TAR FET group) 31...
BACKGROUND: The study intends to analyze influences of different anesthesia and analgesia on cellular immune cognitive functions patients undergoing thoracotomy for esophageal cancer (EsC).METHODS: Patients EsC were divided into four groups: Group A (received general [GA]) postoperative intravenous analgesia); B GA epidural C combined with thoracic [TEA]) D TEA analgesia). T-lymphocyte subsets determined at 30 min before induction (T0), 2 h after skin incision (T1), 4 (T2), 24 (T3), 48 (T4)...
Abstract Duck virus enteritis (DVE) is an acute, contagious herpesvirus infection of ducks, geese, and swans, which has produced significant economic losses in domestic wild waterfowl. With the purpose decreasing commercial duck industry, studying unknown glycoprotein K (gK) DEV may be a new method for preferably preventing curing this disease. So first time to product purify rabbit anti-tgK polyclonal antibody. Through western blot ELISA assay, truncated (tgK) good antigenicity, also...
Glycoprotein K encoded by the DEV-UL53 gene is one of DEV envelope glycoproteins. Like most members alphaherpesvirinae, genes in UL region its genome are well conserved, which means that major glycoprotein possessing similar functions alphaherpesvirinae plays an essential role viral replication and cell fusion. sequence analysis indicates protein possesses typical characteristics membrane glycoproteins containing five transmembrane helix structures NH <sub...
To determine the distribution of duck plague virus (DPV) gE protein in paraformaldehyde-fixed, paraffin-embedded tissues experimentally DPV-infected ducks, an indirect immunoperoxidase assay was established to detect glycoprotein E (gE) for first time. The rabbit anti-His-gE serum, raised against recombinant His-gE fusion expressed Escherichia coli BL21 (DE3), prepared and purified. Western blotting immunofluorescence analysis showed that serum had a high level reactivity specificity could...
Duck enteritis virus (DEV) is an alphaherpesvirinae that causes acute, contagious, and fatal disease. In this study, the DEV-UL53 gene from Key Laboratory of Animal Disease Human Health Sichuan Province was cloned sequenced. Nucleotide sequence analysis revealed a 1032 bp open reading frame (ORF) theoretically encoding 343-amino acid polypeptide homologous to UL53 alphaherpesvirinaes but there just predicted 226aa peptide , which could be expressed facilely,by using GENSCAN. The had base...
Abstract Acute aortic dissection (AAD) is a serious disease characterized by high mortality. However, there are no accurate indicators to predict in-hospital The objective of this study was identify the potential value ischemia modified albumin (IMA) in prediction mortality AAD patients. This single-center, prospective involved 314 patients undergoing AAD, including 197 males and 117 females, aged 26–87 (57.14 ± 21.71) years old, 116 cases TAAD 198 TBAD (37 complicated, 114 risk, 47...