A5057321397- Pharmacological Effects of Natural Compounds
- Food Quality and Safety Studies
- Food composition and properties
- Traditional Chinese Medicine Analysis
- Natural product bioactivities and synthesis
- Seed and Plant Biochemistry
- Genetically Modified Organisms Research
- Chromatography in Natural Products
- Phytochemicals and Antioxidant Activities
- Flavonoids in Medical Research
- Phytochemistry and Biological Activities
Xinjiang Medical University
2024
Fujian Agriculture and Forestry University
2011
Rosa laxa Retz. is an unexplored Rosaceae plant in Xinjiang, China, and its flower traditionally used Kazak to treat the common cold, fever, epileptic seizures lessen effects of aging. In present study, pharmacognostic profiles, physicochemical properties, phytochemical characteristics, vitro antioxidant potency flos (RLF) were presented. evaluation RLF, organoleptic internal structures, powder information observed, parameters, including moisture content, ash, pH value, swelling degree,...
摘要： 蛋白质提取方法和电泳条件是蛋白质组学研究中双向电泳的关键。本文初步建立起一套适合圆果种黄麻功能叶总蛋白双向电泳(2-DE)分析的高效提取方法及电泳条件，在双向电泳技术中，比较了TCA丙酮、Tris-base/丙酮、Tris-HCl的蛋白提取方法，并优化了上样量和样品制备方法。结果表明, TCA丙酮法提取蛋白质产量高，达79.0 mg g–1，比Tris-base/丙酮法和Tris-HCl的产量分别高出44.2%和114.1%。该法得到的2-DE图谱背景清晰，蛋白点数最多，约602个，较其他两种方法更适合用于黄麻功能叶总蛋白双向电泳分析；350 mg为最佳上样量。这为黄麻蛋白质组学后续研究奠定了基础。