A5054464125- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Neurogenetic and Muscular Disorders Research
- Molecular Biology Techniques and Applications
- Pluripotent Stem Cells Research
- Effects of Radiation Exposure
- Cerebral Palsy and Movement Disorders
- Birth, Development, and Health
- Radiation Therapy and Dosimetry
- Radiation Effects and Dosimetry
- Neonatal and fetal brain pathology
- Zebrafish Biomedical Research Applications
- RNA regulation and disease
- Biomedical Ethics and Regulation
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Carcinogens and Genotoxicity Assessment
- Viral Infectious Diseases and Gene Expression in Insects
- Neonatal Respiratory Health Research
- Prenatal Substance Exposure Effects
- Advanced Neuroimaging Techniques and Applications
- Neuroscience and Neural Engineering
- Central Venous Catheters and Hemodialysis
- Pediatric Pain Management Techniques
- Mesenchymal stem cell research
PerkinElmer (United States)
2022
Wayne State University
1999-2014
Children's Hospital of Michigan
2001-2014
Detroit Medical Center
2001
University of Wisconsin–Madison
1996-1999
Albert Einstein College of Medicine
1996-1997
Ethanol (ETOH) consumption by pregnant women can result in Fetal Alcohol Spectrum Disorder (FASD). To date, the cellular targets and mechanisms responsible for FASD are not fully characterized. Our aim was to determine if ETOH affect fetal human brain-derived neural progenitor cells (NPC).Neural were isolated positive selection from normal second trimester brains (n = 4) cultured, up 72 hours, mitogenic media containing 0, 1, 10, or 100 mM ETOH. From 48 hours culture, neurospheres generated...
Defensins are cationic peptides with broad‐spectrum antimicrobial activity. They members of a supergene family consisting α and β subtypes each subtype is comprised number different isoforms. For example, human α‐defensin (HAD) has six isoforms, which expressed by polymorphonuclear leukocytes Paneth cells. In contrast, β‐defensin (HBD) two isoforms that epithelial cells the skin, gut, respiratory urogenital tracts. Recently, HBD‐1 was detected in brain biopsy tissue. However, little known...
Loss-of-function mutations in the genes encoding PINK1 and PRKN result early-onset Parkinson disease (EOPD). Together encoded enzymes direct a neuroprotective pathway that ensures elimination of damaged mitochondria via autophagy. We performed genome-wide high content imaging miRNA screen for inhibitors PINK1-PRKN identified all three members family 29 (miR-29). Using RNAseq we target found siRNA against ATG9A phenocopied effects miR-29 inhibited initiation mitophagy. Furthermore, discovered...
Objectives: To evaluate the effect of oral sucrose on skin blood flow (SBF; perfusion units; PU) measured by Laser Doppler Imager (LDI) in term newborns and pain response (Neonatal Infant Pain Scale score; NIPS score) during heel lance; (2) determine SBF changes (3) relationship between NIPS. Materials Methods: Term infants ≤7 days old (n=56) undergoing routine lance were randomized to pretreatment with 2.0 mL 24% (n=29) or sterile water (n=27) a double-blinded, placebo-controlled trial. was...
The development of safe crop protection products is a complex process that traditionally relies on intensive animal use for hazard identification. Methods capture toxicity in early stages agrochemical discovery programs enable more efficient and sustainable product pipeline. Here, we explored whether the zebrafish model can be leveraged to identify mammalian-relevant toxicity. We used transgenic assess developmental following exposures known mammalian teratogens captured larval morphological...
Rapid and reliable methods for performing biological dosimetry are of paramount importance in the event a large-scale nuclear event. Traditional approaches lack requisite rapid assessment capability, ease use, portability low cost, which factors needed triaging large number victims. Here we describe results experiments mice were acutely exposed to 60Co gamma rays at doses 0 (control) 10 Gy. Blood was obtained from irradiated 0.5, 1, 2, 3, 5, 7 days after exposure. mRNA expression levels 106...
In a large-scale nuclear incident, many thousands of people may be exposed to wide range radiation doses. Rapid biological dosimetry will required on an individualized basis estimate the exposures and make treatment decisions. To ameliorate adverse effects exposure, victims treated with one or more cytokine growth factors, including granulocyte colony-stimulating factor (G-CSF), which has therapeutic efficacy for treating radiation-induced bone marrow ablation by stimulating granulopoiesis....
A comprehensive evaluation of myelination during normal human development is essential to understand the pathology congenital diseases white matter. The present study establishes quantitative values for oligodendrocyte-specific gene expression early stages in fetal spinal cord. Complementary techniques Northern and immunoblotting were used determine relative amounts mRNAs proteins between 12 24 gestational weeks. Values determined myelin basic protein, 2',3'-cyclic nucleotide...
Enriched cultures of human oligodendrocytes were obtained from fetal brain specimens between 16 and 21 gestational weeks. Brain cells separated over a Percoll density gradient collected as two fractions with initial relative densities approximately 1.035 g/ml 1.102 g/ml, for 1 2, respectively. After separation, 58.3 67.7% the in respectively, labeled by antibody O4 that recognizes immature oligodendrocytes. A total 15.5 29.4% positive tubulin-βIII, marker neurons but none freshly isolated...
The isolation culturing and expansion of human neural progenitors cells has important potential clinical applications in cellular transplantation strategies as well developmental studies involving the central nervous system (CNS). This study describes an efficient method to culture neurons astrocytes primary cultures, from proliferative progenitor derived second trimester fetal CNS tissue. Second tissue was mechanically dissociated subjected trypsindissociation trituration. resulting...
The amount of myelin basic protein (MBP) was quantified in human fetal spinal cords from 12 to 24 gestational weeks (GW). MBP expression determined by Northern blot, quantitative immunoblot, and immunocytochemistry. development compact analyzed electron microscopy. Thirty-eight were obtained after elective termination intrauterine pregnancies healthy women. blot analysis showed a 15.8-fold increase mRNA between 18 GW. From GW, increased 2.2-fold. data paralleled immunoblot results that...
Human induced pluripotent stem cell (iPSC)-derived neurons are being increasingly used for high content imaging and screening. However, iPSC-derived neuronal differentiation maturation is time-intensive, often requiring >8 weeks. Unfortunately, the differentiating maturing cultures also tend to migrate coalesce into ganglion-like clusters making single-cell analysis challenging, especially in miniaturized formats. Using our defined extracellular matrix low oxygen culturing conditions of...
Enriched cultures of human oligodendrocytes were obtained from fetal brain specimens between 16 and 21 gestational weeks. Brain cells separated over a Percoll density gradient collected as two fractions with initial relative densities approximately 1.035 g/ml 1.102 g/ml, for 1 2, respectively. After separation, 58.3 67.7% the in respectively, labeled by antibody O4 that recognizes immature oligodendrocytes. A total 15.5 29.4% positive tubulin-βIII, marker neurons but none freshly isolated...
The amount of myelin basic protein (MBP) was quantified in human fetal spinal cords from 12 to 24 gestational weeks (GW). MBP expression determined by Northern blot, quantitative immunoblot, and immunocytochemistry. development compact analyzed electron microscopy. Thirty-eight were obtained after elective termination intrauterine pregnancies healthy women. blot analysis showed a 15.8-fold increase mRNA between 18 GW. From GW, increased 2.2-fold. data paralleled immunoblot results that...