A5026443169- CAR-T cell therapy research
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- CRISPR and Genetic Engineering
- Listeria monocytogenes in Food Safety
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Neurogenesis and neuroplasticity mechanisms
- Microbial Inactivation Methods
- Nanowire Synthesis and Applications
- Cellular Mechanics and Interactions
- Essential Oils and Antimicrobial Activity
- Transgenic Plants and Applications
- Cell Adhesion Molecules Research
- Lipid Membrane Structure and Behavior
- Toxin Mechanisms and Immunotoxins
- Biosimilars and Bioanalytical Methods
- Caveolin-1 and cellular processes
- Neuroinflammation and Neurodegeneration Mechanisms
- Congenital gastrointestinal and neural anomalies
- Nerve injury and regeneration
- Olfactory and Sensory Function Studies
- Biosensors and Analytical Detection
- Salmonella and Campylobacter epidemiology
University of Helsinki
2022-2025
University of Edinburgh
2023
University of Auckland
2009-2017
University of Bristol
2005-2007
Universidad de León
2005-2007
University of Saskatchewan
2005
Abstract Engineered T cell therapies have emerged as a promising approach for cancer treatment, yet their application to solid tumors remains challenging due the limited specificity and persistence of current antigen recognition strategies. Here, we introduced sherpabodies, engineered from human SH3 domain scaffold, class antibody-mimetic proteins capable precise tumor-associated recognition. A phage display library identified sherpabodies against panel popular antigens (TAA), which were...
ABSTRACT We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of target hly gene fragment and an internal amplification control (IAC). The IAC is chimeric double-stranded DNA containing rapeseed BnACCg8 flanked by -specific sequences. This detected using second TaqMan probe labeled with different fluorophore, enabling simultaneous monitoring signals. -IAC had specificity sensitivity 100%, as assessed 49 L. isolates serotypes 96...
The ability to culture neural progenitor cells from the adult human brain has provided an exciting opportunity develop and test potential therapies on cells. To achieve a reliable reproducible cell (AhNPC) system for this purpose, study fully characterized cellular composition of AhNPC cultures, as well possible changes in vitro over prolonged periods. We isolated neurogenic subventricular zone/hippocampus (SVZ/HP) found heterogeneous population comprised several types post-mitotic (neurons,...
Abstract The human brain is a highly vascular organ in which the blood-brain barrier (BBB) tightly regulates molecules entering brain. Pericytes are an integral cell type of BBB, regulating integrity, neuroinflammation, angiogenesis and wound repair. Despite their importance, identifying pericytes amongst other perivascular types deciphering specific role neurovasculature remains challenge. Using primary adult cultures fluorescent-activated sorting, we identified two CD73 + CD45 −...
Chimeric antigen receptor (CAR) T-cell immunotherapies for solid tumors face critical challenges such as heterogeneous expression. We characterized stage-specific embryonic antigen-4 (SSEA-4) cell-surface glycolipid a target CAR therapy. SSEA-4 is mainly expressed during embryogenesis but also found in several cancer types making it an attractive tumor-associated antigen. Anti-SSEA-4 CAR-T cells were generated and assessed preclinically vitro vivo antitumor response safety. effectively...
Summary Listeria ivanovii differs from the human pathogen monocytogenes in that it specifically affects ruminants, causing septicaemia and abortion but not meningo‐encephalitis. The genetic characterization of spontaneous L. mutants lacking virulence factor SmcL (sphingomyelinase) led us to identify LIPI‐2, first species‐specific pathogenicity island . Besides SmcL, this 22 kb chromosomal locus encodes 10 internalin (Inl) proteins: i‐InlB1 ‐B2 are large/surface‐associated Inls similar InlB;...
<p>Figure S5. Cytotoxic activity of Sb CARs expressed in human primary CD8+ T cells (related to Figure 2E)</p>
<p>Figure S2. Jurkat T cell reporter activation (NF-kB-ECFP) with plastic-immobilized target proteins (related to Figure 2B).</p>
<p>Figure S15. Gating strategy to measure intratumoral T cell expansion and CAR expression in a mouse model (related figure 5D).</p>
<p>Figure S14. HCC1569 breast tumor volume measurements of individual mice treated with T cells expressing constitutive or inducible Sb1205 anti-HER2 CAR (related to Figure 5B).</p>
<p>Figure S3. Antigen expression and HER2 knock-down in a panel of cancer cell lines additional Jurkat T reporter activation (NFAT-GFP) assays co-culture with 293T* (HER2 KD) cells transfected tumor associated antigens (related to Figure 2C, 2D.</p>
<p>Figure S8. In vitro and in vivo activity of low a7inity scFv-guided CAR (KD = 3910 nM).</p>
<p>Figure S9. Tumor volume measurement of individual mice treated with T cells expressing Sb1205 anti-HER2 CAR (related to Figure 4C and E).</p>
<p>Figure S4. NFAT-EGFP reporter activation in Jurkat T cells exposed to phorbol myristateacetate (PMA) and ionomycin or co-cultured with target expressing the cognate tumor associated antigen.</p>
<p>Figure S12. Statistical analysis of fraction CD62L+CD45RA+ in T cells engineered with constitutive ScFv and SbCAR inducible (related to Figure 5A).</p>
<p>Figure S11. Gating strategy to define % CD62L+CD45RA+ T cells (related Figure 5A).</p>
<p>Supplementary Figure 1. Binding specificity of sherpabodies against a panel promiscuouslyinteracting macromolecules</p>
<div>Abstract<p>Engineered T-cell therapies have emerged as a promising approach for cancer treatment, yet their application to solid tumors remains challenging because of the limited specificity and persistence current antigen recognition strategies. In this study, we introduce sherpabodies, engineered from human SH3 domain scaffold, class antibody-mimetic proteins capable precise tumor-associated (TAA) recognition. A phage display library identified sherpabodies against panel...
<p>Figure S10. Luminescence measurements of T cells expressing E:Luc reporter (related for Figure 4D).</p>
<p>Nucleotide sequences of constructs used in the study</p>
<p>Figure S6. Cytokine secretion of SbCARs expressed in human primary CD8 T cells (related to Figure 2E).</p>
<p>Figure S13. SKOV3 ovarian tumor volume measurements of individual mice treated with T cells expressing constitutive or inducible Sb1205 anti-HER2 CAR (related to Figure 5B and C).</p>
<p>Figure S7. Jurkat T cell reporter activation (NFAT-EGFP) for multispecific SbCARs in cocultures with a panel of cancer lines.</p>