- Plant biochemistry and biosynthesis
- Viral gastroenteritis research and epidemiology
- Polyamine Metabolism and Applications
- Essential Oils and Antimicrobial Activity
- Advanced Software Engineering Methodologies
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Food Quality and Safety Studies
- Machine Learning and Algorithms
- Hepatitis Viruses Studies and Epidemiology
- RNA and protein synthesis mechanisms
- Plant Pathogenic Bacteria Studies
- Protein Hydrolysis and Bioactive Peptides
- Photosynthetic Processes and Mechanisms
- Amino Acid Enzymes and Metabolism
- Plant Gene Expression Analysis
- Sesquiterpenes and Asteraceae Studies
- Service-Oriented Architecture and Web Services
- Plant nutrient uptake and metabolism
- Plant Reproductive Biology
- Liver Diseases and Immunity
- Cytomegalovirus and herpesvirus research
- Software Engineering Techniques and Practices
- Algorithms and Data Compression
- Machine Learning and Data Classification
Central Institute of Medicinal and Aromatic Plants
2022-2024
Bharat Heavy Electricals (India)
2024
University of St. Gallen
1981
Board of the Swiss Federal Institutes of Technology
1979
Washington University in St. Louis
1973
Central Food Technological Research Institute
1966-1971
A solid-phase enzyme immunoassay for the determination of immunoglobulin H (IgG) and IgM antibodies to cytomegalovirus is described. The gave reliable consistent results which were in concordance with those obtained by complement fixation test indirect immunofluorescence test. Antibodies herpes simplex varicella-zoster viruses did not interfere antibodies. In a few patients Epstein-Barr virus, also detected. False-positive antibody observed sera containing both rheumatoid factor IgG This...
A gamma-glutamyl kinase (ATP-L-glutamate 5-phosphotransferase) was purified about 85-fold from crude extracts of Pseudomonas aeruginosa strain PAO 1 by (NH4)2SO4 precipitation, molecular-sieving Sephadex G-150 and DEAE-cellulose chromatography. The molecular weight this enzyme 84,000. preparation catalysed formation hydroxamate L-glutamate, ATP Mg2+ or Mn2+ with concomitant hydrolysis to ADP + Pi. L-Proline inhibited the activity 50% at 5 mM almost completely 30 mM. inhibition L-proline...
1. Cell-free extracts of Escherichia coli K12 catalyse the synthesis N-acetyl-l-phenylalanine from acetyl-CoA and l-phenylalanine. 2. The acetyl-CoA–l-phenylalanine α-N-acetyltransferase was purified 160-fold cell-free extracts. 3. enzyme has a pH optimum 8 catalyses acetylation Other l-amino acids such as histidine alanine are acetylated at slower rates. 4. A transacylase also E. its substrate specificity studied. 5. properties both these enzymes were compared with those other known amino...
Terpene synthases (TPSs) have diverse biological functions in plants. Though the roles of TPSs herbivore defense are well established many plant species, their role bacterial has been scarce and is emerging. Through functional genomics, here we report planta potato (Solanum tuberosum) terpene synthase (StTPS18) defense. Expression StTPS18 was highest leaves induced response to Pseudomonas syringae methyl jasmonate treatments. The recombinant exhibited bona fide (E,E)-farnesol activity...
SUMMARY Citral, a naturally occurring acyclic monoterpene aldehyde, is present in the essential oils of various plants, but only few produce it abundance. Despite its importance as key aroma molecule, knowledge regarding in‐planta biosynthesis citral and metabolic origin remains limited. Here, we have elucidated functions an alcohol dehydrogenase (CfADH1) aldoketo‐reductase (CfAKR2b) lemongrass ( Cymbopogon flexuosus ), one most cultivated aromatic crops for citral‐rich oil. Expression both...
Abstract Except for the genetic basis of citral-forming alcohol dehydrogenases (ADHs) in Litsea cubeba tree, and biochemical studies on enzymes from select plants, knowledge regarding in-planta biosynthesis citral its metabolic origin remains limited. Here, we have elucidated functions an ADH (CfADH1) aldoketo-reductase (CfAKR2b) lemongrass ( Cymbopogon flexuosus ), one most cultivated aromatic crops citral-rich essential oil. Expression both CfADH1 CfAKR2b showed correlation with...