A Regulatory Element Is Characterized by Purine-Mediated and Cell-Type-Specific Gene Transcription:

• DOI: 10.1128/mcb.10.10.5600-b
• Publication Date: 2015-10-01T21:59:57Z
• Authors: Kui Zhao; Xiaohui Cheng; Zhenyu Ye; Yecheng Li; Wei Peng; Yongyou Wu; Chungen Xing; Liam Baird; Masayuki Yamamoto; Robert A. Waterland; Randy L. Jirtle; Joyce van de Leemput; Zhe Han; Richard V. Parry; Jens M. Chemnitz; Kenneth A. Frauwirth; Anthony R. Lanfranco; Inbal Braunstein; Sumire Kobayashi; Peter S. Linsley; Craig B. Thompson; James L. Riley; Philippe P. Roux; Ivan Topisirović; Steffen Jung; Júlio Aliberti; Petra Graemmel; Mary Jean Sunshine; Georg W. Kreutzberg; Alan Sher; Dan R. Littman; Nhien Tran; Aaron Broun; Kai Ge; Jiannan Guo; Matthew Garrett; Gos Micklem; Saverio Brogna; Chi V. Dang; Corrine Corrina R...; Ashish Lal; Mark J. Demma; Claudio Mapelli; Angie Sun; Smaranda Bodea; Benjamin Ruprecht; Sarah Javaid; Derek Wiswell; Eric S. Muise; Shiying Chen; John Zelina; Federica Orvieto; Alessia Santoprete; Simona Altezza; Federica Tucci; Enrique Escandón; Brian E. Hall; Kallol Ray; Abbas M. Walji; Jennifer O’Neil; Sebastian Alers; Antje S Löffler; Sebastian Wesselborg; Björn Stork; Kaifu Chen; Wang Jian-hu; Xia Zheng; Dongyu Zhao; Wei Li; Jessica K. Tyler; Eek‐hoon Jho; Tong Zhang; Claire Domon‐Dell; Choun‐Ki Joo; Jean‐Noël Freund; Frank Costantini; Steven P. Gygi; Yvan Rochon; B. Robert Franza; Ruedi Aebersold; Judith Lopes; Cyril Ribeyre; Alain Nicolas; Ji‐Eun Lee; Hannah Schmidt; Binbin Lai; Tayeba Khan; Eric S. Muise; Puneeth Iyengar; Zhao V. Wang; Manisha Chandalia; Nicola Abate; Bei B. Zhang; Paolo Bonaldo; Streamson C. Chua; Philipp E. Scherer; Cheng‐Jun Hu; Li-Yi Wang; Lewis A. Chodosh; Brian Keith; M. Celeste Simon
• Keywords: Exosome

Exosomes are microvesicles secreted by body cells for intercellular communication. The circular RNA circ_0000338 was found to be present in extracellular vesicles and improve the chemoresistance of colorectal cancer (CRC) cells. However, role exosomal 5-fluorouracil (5-FU) resistance CRC is largely unknown. levels circ_0000338, microRNA 217 (miR-217), miR-485-3p were detected using quantitative real-time PCR (qRT-PCR). 50% inhibitory concentration (IC50) values 5-FU, cell proliferation, apoptosis evaluated counting kit 8 (CCK-8), colony formation, flow cytometry, Western blot assays. interaction between miR-217 or confirmed immunoprecipitation (RIP), dual-luciferase reporter, pulldown isolated ultracentrifugation qualified transmission electron microscopy (TEM), Nanosight tracking analysis (NTA), blotting. Xenograft models performed analyze whether circ_0000338-loaded exosomes could increase 5-FU vivo. level elevated 5-FU-resistant tissues cells, knockdown sensitized through enhancing decreasing proliferation vitro. Mechanistically, directly bound miR-485-3p, inhibition reversed effects on CRC. Additionally, incorporated into transmitted 5-FU-sensitive Treatment-sensitive with containing reduced response both vitro Besides that, higher patients exhibiting showed good diagnostic efficiency delivery via enhanced negative regulation indicating a promising therapeutic marker 5-FU-based chemotherapy patients.