A5100703518- Circular RNAs in diseases
- Virus-based gene therapy research
- MicroRNA in disease regulation
- Glycosylation and Glycoproteins Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- 14-3-3 protein interactions
- Ubiquitin and proteasome pathways
- Inflammatory Bowel Disease
- Radiomics and Machine Learning in Medical Imaging
- Ferroptosis and cancer prognosis
- Cancer-related gene regulation
- Bacteriophages and microbial interactions
- Cancer Mechanisms and Therapy
- Autophagy in Disease and Therapy
- Extracellular vesicles in disease
- Mesenchymal stem cell research
- Pancreatic and Hepatic Oncology Research
- Virology and Viral Diseases
- Advanced Proteomics Techniques and Applications
- Cancer Research and Treatments
- Genetic Syndromes and Imprinting
- Cell death mechanisms and regulation
- Health Sciences Research and Education
- Gallbladder and Bile Duct Disorders
The First Affiliated Hospital, Sun Yat-sen University
2024-2025
Sun Yat-sen University
2024-2025
Second Affiliated Hospital of Soochow University
1989-2024
Soochow University
1989-2024
University of Chicago Medical Center
2018-2019
Molecular Oncology (United States)
2018-2019
Marshfield Clinic
2013-2014
University of Virginia
1994-1995
Mesenchymal stem cells (MSCs) are ubiquitously-existing multipotent progenitors that can self-renew and differentiate into multiple lineages including osteocytes, chondrocytes, adipocytes, tenocytes myocytes. MSCs represent one of the most commonly-used adult serve as excellent progenitor cell models for investigating lineage-specific differentiation regulated by various cellular signaling pathways, such bone morphogenetic proteins (BMPs). As members TGFβ superfamily, BMPs play diverse...
ABSTRACTExosomes are microvesicles secreted by body cells for intercellular communication. The circular RNA circ_0000338 was found to be present in extracellular vesicles and improve the chemoresistance of colorectal cancer (CRC) cells. However, role exosomal 5-fluorouracil (5-FU) resistance CRC is largely unknown. levels circ_0000338, microRNA 217 (miR-217), miR-485-3p were detected using quantitative real-time PCR (qRT-PCR). 50% inhibitory concentration (IC50) values 5-FU, cell...
Pancreatic ductal adenocarcinoma (PDAC) is one of the most deadly malignancies with <5% five-year survival rate due to late diagnosis, limited treatment options and chemoresistance. There thus an urgent unmet clinical need develop effective anticancer drugs treat pancreatic cancer. Here, we study potential repurposing monensin as drug for chemo-resistant Using two commonly-used cancer cell lines PANC-1 MiaPaCa-2, show that suppresses proliferation migration, cycle progression, while solicits...
The matrix protein M1 of influenza virus A/WSN/33 was shown by immunofluorescent staining to be transported into the nuclei transfected cells without requiring other viral proteins. We postulated existence a potential signal sequence at amino acids 101 105 (RKLKR) that is required for nuclear localization protein. When CV1 were with recombinant vectors expressing entire (amino 1 252) or just first 112 N-terminal acids, both complete and truncated nucleus. In contrast, expression in coding...
MicroRNAs (miRNAs) are ~22 nucleotide noncoding RNAs that involved in virtually all aspects of cellular process as their deregulations associated with many pathological conditions. Mature miRNAs (mMIRs) generated through a series tightly-regulated nuclear and cytoplasmic processing events the transcribed primary, precursor mMIRs. Effective manipulations miRNA expression enable us to gain insights into functions explore potential therapeutic applications. Currently, overexpression is achieved...
Transfection of mammalian CV1 cells with a recombinant M-gene pTM1 plasmid, driven by vaccinia virus-expressed phage T7 polymerase, resulted in the expression matrix (M) protein, which is progressively released from exterior surface transfected-cell plasma membrane. Exocytosis M protein begins 2 to 4 h posttransfection and reaches peak 10 16 posttransfection; dye uptake studies reveal that > 97% are alive have intact membranes at posttransfection. Density gradient centrifugation labeling...
Herpes simplex virus type 1 (HSV-1) capsids were found to assemble spontaneously in a cell-free system consisting of extracts prepared from insect cells that had been infected with recombinant baculoviruses coding for HSV-1 capsid proteins. The formed this resembled native morphology as judged by electron microscopy, sedimentation rate on sucrose density gradients, protein composition, and their ability react antibodies specific the major protein, VP5. Optimal assembly required presence...
Disulfidptosis, a regulated form of cell death, has been recently reported in cancers characterized by high SLC7A11 expression, including invasive breast carcinoma, lung adenocarcinoma, and hepatocellular carcinoma. However, its role colon adenocarcinoma (COAD) infrequently discussed. In this study, we developed validated prognostic model based on 20 disulfidptosis-related genes (DRGs) using LASSO Cox regression analyses. The robustness practicality were assessed via nomogram. Subsequent...
Abstract Background Crohn’s disease (CD) is a chronic inflammatory condition of the gastrointestinal tract, and while biologics like infliximab (IFX) offer significant benefits, 10-30% patients remain non-responders initially. This study aims to refine prediction treatment responses using artificial intelligence integrate multimodal information, potentially enhancing precision. Methods We conducted retrospective analysis clinical data from treated at The First Affiliated Hospital Sun Yat-sen...
Increasing evidence has demonstrated that long non‑coding (lnc) RNA is aberrantly expressed in numerous types of cancer. Colorectal cancer a common malignancy; however, the role and mechanism underlying influence lncRNA‑colon associated transcript 1 (CCAT1) colorectal yet to be elucidated. The present study revealed CCAT1 highly tissues. Bioinformatics analysis dual‑luciferase reporter gene assay indicated microRNA (miR)‑218 had complementary binding sites. Furthermore, reverse...
The membrane-binding affinity of the matrix (M) protein vesicular stomatitis virus (VSV) was examined by comparing cellular distribution wild-type (wt) M with that temperature-sensitive (ts) and deletion mutants probed indirect fluorescent-antibody staining fractionation infected or plasmid-transfected CV1 cells. M-gene mutant tsO23 caused cytopathic rounding cells at permissive temperature but not nonpermissive temperature; wt VSV also causes rounding, which prohibits study staining. Little...
Abstract Background Acute liver injury (ALI) is an essential component of sepsis associated with poor outcomes. Octanoic acid (OA), a medium‐chain fatty acid, has protective effect on sepsis‐induced organ damage, and autophagy adaptive response to sepsis. However, the underlying mechanism by which OA prevents ALI remains unknown. Therefore, we investigated whether OA‐rich enteral nutrition (EN) prevented lipopolysaccharide (LPS)‐induced through c‐Jun N‐terminal kinase (JNK)‐dependent...
Eric R. Anderson, DO*⇑1, Zhan Ye, PhD†, Michael D. Caldwell, MD, PhD‡ and James K. Burmester, PhD§,2 *Surgical Resident, Marshfield Clinic, Marshfield, WI USA †Biomedical Informatics Research Center, Clinic Foundation, Wisconsin, ‡Department of General Surgery, Director Wound Healing Program, §Clinical Correspondending Author: DO; Department Surgery; Divine Savior Health Care; 2817 New Pinery Rd; Portage 53901 USA; Tel: 608-742-4131; Email: eanderson{at}dshealthcare.com
Colorectal carcinoma (CRC) is one of the most common cancers, and associated with a poor clinical outcome. The key genes potential prognostic markers in colorectal remain to be identified explored for application. DNA expression/methylation profiles were downloaded from Gene Expression Omnibus (GEO) database identify differentially expressed/methylated (DEGs DEMs). A total 255 372 as being up-regulated down-regulated, respectively, GSE113513, GSE81558, GSE89076. There 3350 hypermethylated...
This study aimed to investigate the clinical efficacy of two surgical methods on hyperparathyroidism secondary uremia and summarize advantages disadvantages both methods.Uremic patients who received parathyroidectomy (PTX) in last 3 years were divided into groups according used [subtotal (SPTX) group total + autologous implantation (TPTX AT) group]. TPTX was performed if less than 4 glands found during surgery. The changes various indexes after operation, calculate success rate recurrence...
Abstract Background Predicting primary non-response to infliximab (IFX) is important select the optimal therapy and utilize personalized management in patients of Crohn’s disease (CD). This study aimed develop validate a deep learning (DL) model based on histological characteristics for predicting IFX CD. Methods A training cohort validation were recruited from First Affiliated Hospital Sun Yat-sen University. Clinical features, serological biomarkers, imaging features colonoscopy biopsy at...