The diagnostic value of automated quantitative DNA cytometry for pancreatic malignancy

• DOI: 10.3760/cma.j.issn.1007-5232.2018.03.002
• Publication Date: 2018-03-20
• Authors: Xiaoyin Zhang; Min Zhao; Xin Fu; Yang Li; Qiao Yang; Na Liu; Changcun Guo; Xiaoru Ke; Xin Wang; Guo Xuegang; Kaichun Wu; Daiming Fan
• Keywords: Youden's J statistic; Cytometry

Objective To estimate the diagnostic value of cytology, DNA-ICM (DNA-image cytometry), cytology combined with for pancreatic malignancy, and to explore cut-off DNA-ICM.

Methods Patients suspicious malignancy were retrospectively identified. In total, 145 EUS-FNA specimens acquired from 140 separate patients examined by DNA-ICM. Diagnostic values among combination techniques in detecting compared.

Results Compared had a lower sensitivity (63.0% VS 82.4%) accuracy (69.7% 85.5%). After combining techniques, significantly improved compared that (0.941 0.912, P=0.007 0) or only 0.815, P<0.000 1). By using Youden index, detect was one cell DI (DNA index)≥2.5. Notably, this standard, increased 72.3% 77.2%, those 91.6% 93.1%, respectively.

Conclusion Automated is an objective effective method malignancy. Although has than conventional obtained after techniques.

Key words: Pancreatic neoplasms; Pathology; DNA probes; Cytodiagnosis