Scope Due to the low bioavailability of resveratrol, determining whether its metabolites exert any beneficial effect is an interesting issue. Methods and results 3 T 3‐ L 1 maturing pre‐adipocytes were treated from day 0 8 differentiation mature adipocytes for 24 h on 12 with 1, 10, or 25 μM resveratrol metabolites. Triacylglycerols assessed by spectrophotometry gene expression real time RT‐PCR. Resveratrol, trans ‐resveratrol‐4′‐ O ‐glucuronide ‐resveratrol‐3‐ ‐sulfate reduced...

This study was designed to analyze the anti-adipogenic effect of fifteen phenolic compounds from various chemical groups in 3T3-L1 pre-adipocytes. Cells were treated with 25 μM, 10 μM or 1 apigenin, luteolin, catechin, epicatechin, epigallocatechin, genistein, daizein, naringenin, hesperidin, quercetin, kaempferol, resveratrol, vanillic acid, piceatannol and pterostilbene for 8 days. At lipid accumulation reduced by all compounds, exception epicatechin epigallocatechin. a dose quercetin...

Due to the low bioavailability of resveratrol, determining whether its metabolites exert any beneficial effect is an interesting issue.3T3-L1 maturing pre-adipocytes were treated during differentiation with 25 µM resveratrol or and 3T3-L1 mature adipocytes for 24 hours 10 metabolites. The gene expression adiponectin, leptin, visfatin apelin was assessed by Real Time RT-PCR their concentration in incubation medium quantified ELISA.Resveratrol reduced mRNA levels leptin increased those...

Scope . To determine whether doses of quercetin in the range serum concentrations exert any effect on triacylglycerol accumulation maturing preadipocytes and mature adipocytes. The influence expression adipogenic markers as well gene activity enzymes involved metabolism were assessed. Methods Results 3T3-L1 treated during differentiation adipocytes for 24 hours with low (0.1–10 µ M) quercetin. Triacylglycerol content both cell types free fatty acid glycerol incubation medium measured...

Abstract Opuntia ficus-indica fruits have been widely used due to their nutritional composition and beneficial effects on health, particularly against chronic diseases such as diabetes, obesity, cardiovascular cancer, among others. In recent years, prickly pear peel pulp extracts characterised, a high number of bioactive compounds identified. This study aimed analyse the triglyceride-lowering effect obtained from three varieties (Pelota, Sanguinos, Colorada) in 3T3-L1 maturing mature...

Proposed miRNA mechanisms of action resveratrol in triacylglycerol metabolism changes adipose tissue.

Objective Scientific research is constantly striving to find molecules which are effective against excessive body fat and its associated complications. Taking into account the beneficial effects that resveratrol exerts on other pathologies through miRNA, aim of present work was analyze possible involvement miRNAs in regulation adipogenic transcription factors peroxisome proliferator-activated receptor γ (pparγ), CCAAT enhancer-binding proteins α β (cebpβ cebpα) induced by metabolites....

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The aim of the present study was to determine whether reduction in liver fat previously observed our laboratory a cohort rats which had been fed an obesogenic diet mediated by changes expression microRNA (miRNA)-103-3p, miRNA-107-3p and miRNA-122-5p, represent 70% total miRNAs liver, as well their target genes. three analysed reduced treated with resveratrol. A sterol-regulatory element binding protein 1 (SREBP1) increase carnitine palmitoyltransferase 1a (CPT1a) were resveratrol-treated...

Quercetin (Q) has rapid metabolism, which may make it worthwhile to focus on the potential activity of its metabolites. Our aim was evaluate triglyceride-lowering effects Q metabolites in mature and pre-adipocytes, compare them those induced by Q. 3T3-L1 pre-adipocytes were treated with 0.1, 1 10 µM Q, tamarixetin (TAM), isorhamnetin (ISO), quercetin-3-O-glucuronide (3G), quercetin-3-O-sulfate (3S), as well 3S quercetin-4-O-sulfate (4S) mixture (3S+4S). Triglyceride (TG) content both cell...

Trans-ε-viniferin, a resveratrol dimer found mainly in grapevine wood, has shown protective capacities against hepatic steatosis vivo. Nevertheless, this compound is very poorly bioavailable. Thus, the aim of present study to determine potential anti-steatotic properties 1 and 10 µM trans-ε-viniferin its four glucuronide metabolites AML-12 cells treated with palmitic acid as an vitro model steatosis. The effect molecules cell viability triglyceride accumulation, underlying mechanisms action...

Phenolic compounds have emerged in recent years as an option to face insulin resistance and diabetes. The central aim of this study was: (1) demonstrate that physiological doses resveratrol (RSV) or quercetin (Q) can influence glucose metabolism human myotubes, (2) establish whether AMP-activated protein kinase (AMPK) B –PKB- (Akt) pathways are involved effect. In addition, the effects these polyphenols on mitochondrial biogenesis fatty acid oxidation were analysed. Myotubes from healthy...

Opuntia stricta var. dillenii, a member of the Cactaceae family, produces fruit known as prickly pear. This is rich in bioactive compounds, including betalains and phenolic which play an important role health promotion due to their antioxidant anti-inflammatory properties. study aims investigate impact pear extracts obtained from whole fruit, peel, pulp, industrial by-product (bagasse) on differentiation 3T3-L1 pre-adipocytes. During process, pre-adipocytes were treated with at...

Gibel gantzatsu ez-alkoholikoa (GGEA) espektro zabaleko gaixotasuna da, zeinak gaixotasun-multzo bat hartzen baitu barnean, gibel esteatositik hasi eta gibel-minbiziraino iritsi daitekeena. Haren garapenerako arrisku faktore garrantzitsuenak obesitatea intsulinarekiko erresistentzia dira, gaur egun osasun-arazo pandemikoa izatera direnak. Populazioaren %25ek gibel-esteatosia pairatzen duela uste da: datu hauek gutxi gorabeherakoak asintomatikoa izanik duen diagnosi zaila baita. Prebalentzia...

Minbizia egungo osasun-arazo publiko garrantzitsuenetarikoa da; izan ere, tumoreen intzidentzia izugarri handitu da azken hamarkadetan. Beste faktore askoren artean, elikadura-patroiak nabarmen eragiten du minbiziaren garapenean; hala nola, obesitateak eta haren komorbilitateek. Hainbat minbizi mota garatzeko arriskua areagotzen dute, besteak beste gizon emakumezkoetan tiroide, hestegorri, gibel, behazun-besikula, kolon, giltzurrun mieloma anitzeko minbiziak, bular endometrioko minbiziak...