A5068355941- Botanical Research and Chemistry
- Plant Toxicity and Pharmacological Properties
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Phytochemicals and Antioxidant Activities
- Plant and fungal interactions
- Forensic Toxicology and Drug Analysis
- Cannabis and Cannabinoid Research
- Natural Compound Pharmacology Studies
- Fermentation and Sensory Analysis
- Drug-Induced Hepatotoxicity and Protection
- Alcohol Consumption and Health Effects
- Plant Physiology and Cultivation Studies
- Poisoning and overdose treatments
- Botany, Ecology, and Taxonomy Studies
- Natural product bioactivities and synthesis
- Biochemical and biochemical processes
- Bee Products Chemical Analysis
- Plant and animal studies
- Plant Molecular Biology Research
- Horticultural and Viticultural Research
- Plant Pathogens and Fungal Diseases
- Food Quality and Safety Studies
- Phytochemistry and Biological Activities
Technische Universität Braunschweig
2015-2025
University of Michigan
2002
University of Würzburg
1996-1999
Pernod Ricard (France)
1997
University of Turin
1997
Some basil varieties are able to convert the phenylpropenes chavicol and eugenol methylchavicol methyleugenol, respectively. Chavicol O-methyltransferase (CVOMT) (EOMT) cDNAs were isolated from sweet variety EMX-1 using a biochemical genomics approach. These encode proteins that 90% identical each other very similar several isoflavone O-methyltransferases such as IOMT, which catalyzes 4'-O-methylation of 2,7,4'-trihydroxyisoflavanone. On hand, CVOMT1 EOMT1 related only distantly (iso)eugenol...
Abstract Rose (Rosa hybrida) flowers produce and emit a diverse array of volatiles, characteristic to their unique scent. One the most prominent compounds in floral volatiles many rose varieties is methoxylated phenolic derivative 3,5-dimethoxytoluene (orcinol dimethyl ether). Cell-free extracts derived from developing petals displayedO-methyltransferase (OMT) activities toward several substrates, including 3,5-dihydroxytoluene (orcinol), 3-methoxy,5-hydroxytoluene monomethyl ether),...
Recently, contamination of honey with pyrrolizidine alkaloids (PA) has been reported as potential health risk. Therefore, it was interest to develop a reliable tool for selective and quantitative determination PA in honey. Sample preparation the novel method comprises strong cation exchange SPE (SCX-SPE), followed by two reduction steps using zinc LiAlH(4), well subsequent silylation. During this procedure separated are converted into necin backbone, common structural feature toxicity, which...
Abstract Sweet basil (Ocimum basilicum) peltate glandular trichomes produce a variety of small molecular weight phenylpropanoids, such as eugenol, caffeic acid, and rosmarinic that result from meta hydroxylation reactions. Some lines do not synthesize eugenol but instead chavicol, phenylpropanoid does contain metahydroxyl group. Two distinct acyltransferases,p-coumaroyl-coenzyme A:shikimic acidp-coumaroyl transferase andp-coumaroyl-coenzyme A:4-hydroxyphenyllactic transferase, responsible...
Recently, 1,2-dehydropyrrolizidine alkaloid (PA) ester alkaloids, found predominantly as their N-oxides (PANOs, pyrrolizidine N-oxides), have been reported in both honey and pollen obtained directly from PA plants loads collected by bees, raising the possibility of health risks for consumers these products. We confirm findings regard to floral pollen, using flowers known Senecio jacobaea, S. vernalis, Echium vulgare pollinia Phalaenopsis hybrids, we extend analyses 1,2-unsaturated PAs PANOs...
Pyrrolizidine alkaloids (PAs) are a structurally diverse group of toxicologically relevant secondary plant metabolites. Currently, two analytical methods used to determine PA content in honey. To achieve reasonably high sensitivity and selectivity, mass spectrometry detection is demanded. One method an HPLC-ESI-MS-MS approach, the other sum parameter utilising HRGC-EI-MS operated selected ion monitoring mode (SIM). date, no fully validated or standardised exists measure establish LC-MS...
Synthetic compounds mimicking cannabis‐like effects are a recent trend. Currently, these so‐called synthetic cannabinoids the largest and fastest growing class of newly appearing designer drugs. Many national authorities continuously adapting their regulations to keep pace with permanently changing variety compounds. We have analyzed eight herbal smoking blends containing cannabinoids. Altogether, nine could be identified, namely AM‐2201, AM‐2201‐pMe (MAM‐2201), AM‐1220, AM‐1220‐azepane,...
Plants have evolved a variety of mechanisms for dealing with insect herbivory among which chemical defense through secondary metabolites plays prominent role. Physiological, behavioural and sensorical adaptations to these chemicals provide herbivores selective advantages allowing them diversify within the newly occupied ecological niche. In turn, this may influence evolution plant metabolism giving rise e.g. new defenses. The association Pierid butterflies plants Brassicales has been cited...
Abstract Although a number of plant natural products are derived from benzoic acid, the biosynthesis this structurally simple precursor is poorly understood. Hypericum calycinum cell cultures accumulate acid-derived xanthone phytoalexin, hyperxanthone E, in response to elicitor treatment. Using subtracted complementary DNA (cDNA) library and sequence information about conserved coenzyme A (CoA) ligase motifs, cDNA encoding cinnamate:CoA (CNL) was isolated. This enzyme channels metabolic flux...
Contamination of food and feed with pyrrolizidine alkaloids is currently discussed as a potential health risk. Here, we report the development new HPLC-ESI-MS/MS sum parameter method to quantitate alkaloid content in complex matrices. The procedure was validated for honey culinary herbs. Isotopically labeled 7-O-9-O-dibutyroyl-[9,9-(2)H2]-retronecine synthesized utilized an internal standard validation quantitation. total sample expressed single parameter: retronecine equivalents (RE). Ld/Lq...
Xanthones are natural products present in plants and microorganisms. In plants, their biosynthesis starts with regioselective cyclization of 2,3',4,6-tetrahydroxybenzophenone to either 1,3,5- or 1,3,7-trihydroxyxanthones, catalysed by cytochrome P450 (CYP) enzymes. Here we isolate express CYP81AA-coding sequences from Hypericum calycinum H. perforatum yeast. Microsomes catalyse two consecutive reactions, that is, 3'-hydroxylation 2,4,6-trihydroxybenzophenone C-O phenol coupling the resulting...
The rapid expansion of lithium-ion batteries (LIBs), largely driven by the rising demand for electric vehicles, will lead to a significant increase in end-of-life (EOL) batteries, necessitating efficient recycling processes, which must be accompanied equally purification steps. This study addresses challenge reusing organic electrolyte solvents from spent LIBs, key component often overlooked existing strategies. To address this issue, we developed gas chromatography (GC) method. A variety...
In recent years, there has been a steadily growing number of published data on pyrrolizidine alkaloids (PAs) in honey and pollen. This raises the question whether and/or pollen used as ingredients food processing might provoke downstream contamination chain. Here we addressed two different facets connection with PAs First, analysed PA content several types such mead (n = 19), candy 10), fennel 9), soft drinks 5), power bars cereals 7), jelly babies 3), baby supplements 3) fruit sauce 1) that...
The Rosaceous subtribe Pyrinae (formerly subfamily Maloideae) is well-known for its economically important fruit trees, such as apple and pear, also includes Sorbus aucuparia. Elicitor-treated S. aucuparia cell cultures are used to study the biosynthesis of Pyrinae-specific phytoalexins, biphenyls dibenzofurans. Three (aucuparin, noraucuparin, 2′-hydroxyaucuparin) a dibenzofuran (eriobofuran) were isolated structure elucidated using GC-MS NMR. A second low abundance was tentatively assigned...