A5007442081- Atherosclerosis and Cardiovascular Diseases
- Cholesterol and Lipid Metabolism
- Cancer, Lipids, and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Cell Adhesion Molecules Research
- Lipoproteins and Cardiovascular Health
- Fungal and yeast genetics research
- Prion Diseases and Protein Misfolding
- RNA Research and Splicing
- Fatty Acid Research and Health
- Protease and Inhibitor Mechanisms
- Biomarkers in Disease Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Antiplatelet Therapy and Cardiovascular Diseases
- Peroxisome Proliferator-Activated Receptors
- Platelet Disorders and Treatments
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Cardiovascular Disease and Adiposity
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Metabolomics and Mass Spectrometry Studies
- Nitric Oxide and Endothelin Effects
- Endoplasmic Reticulum Stress and Disease
National Medical Research Center of Cardiology
1981-2021
Institute of Experimental Cardiology
2001-2011
National Research Center for Hematology Russian Academy of Medical Sciences
2007
The Alfred Hospital
1999-2004
Ministry of Health of the Russian Federation
2003
Montana State University
2002
Russian Academy of Sciences
1980-1995
Boston University
1992
Houston Methodist
1992
Academy of Medical Sciences
1980-1986
Mesenchymal stem cells (MSCs) have the capability for renewal and differentiation into various lineages of mesenchymal tissues. These features MSCs attract a lot attention from investigators in context cell-based therapies several human diseases. Despite fact that bone marrow represents main available source MSCs, use marrow-derived is not always acceptable due to high degree viral infection significant drop cell number proliferative/differentiation capacity with age. Thus, search possible...
Abstract The SUP35 gene of yeast Saccharomyces cerevisiae encodes a 76.5-kD ribosome-associated protein (Sup35p), the C-terminal part which exhibits high degree similarity to EF-1 alpha elongation factor, while its N-terminal region is unique. Mutations in or overexpression can generate an omnipotent suppressor effect. In present study wild-type was replaced with deletion alleles generated vitro that encode Sup35p lacking all unique region. These 5'-deletion lead, haploid strain,...
SUP35 is an omnipotent suppressor gene of Saccharomyces cerevisiae coding for a protein consisting C-terminal part similar to the elongation factor EF-1 alpha and unique N-terminal sequence 253 amino acids. Twelve truncated versions were generated by deletion fragments internal sequence. Functional studies these mutants showed that: (i) only alpha-like Sup35 essential cell viability; (ii) overexpression either or full-length decreases translational fidelity, resulting in suppression reduced...
The yeast cytoplasmically inherited genetic determinant [ PSI + ] is presumed to be a manifestation of the prion-like properties Sup35 protein (Sup35p). Here, cell-free conversion Sup35p from psi − cells (Sup35p ) ]-specific form was observed. reaction could repeated for several consecutive cycles, thus modeling in vitro continuous propagation. Size fractionation lysates demonstrated that converting activity associated solely with aggregates, which agrees nucleation model purified and showed...
Vinculin- and caldesmon-immunoreactive forms actin isoform patterns were studied in samples of normal atherosclerotic human aorta. After removal adventitia endothelium, the remaining tissue was divided into three layers: media, muscular-elastic (adjacent to media) intima, subendothelial (juxtaluminal) intima. In media aorta, meta-vinculin accounted for 41.0 +/- 0.9% (mean SEM) total immunoreactive vinculin (meta-vinculin + vinculin); 150-kDa caldesmon 78.2 5.1% (150-kDa 70-kDa); fractional...
Some animal studies suggest that transforming growth factor-beta (TGF-beta) protects vessels from atherosclerosis by preventing intima formation, but others indicate a role in vessel proteoglycan accumulation and lipoprotein retention. To distinguish between these possibilities humans, immunohistochemical were performed examining the coexpression of TGF-beta isoforms receptors ALK-5 TbetaR-II aorta during various stages atherosclerotic lesion development.The spatial relationships TGF-beta1,...
Objective— Despite studies implicating superoxide anion–producing oxidases in atherosclerosis, their characteristics, expression, and regulation cells of lesions are poorly understood. We examined the following: (1) whether cytochrome b 558 –dependent NAD(P)H oxidase–phox peptides expressed by intimal smooth muscle (iSMCs) macrophages human aortic atherosclerotic (2) oxidase represents a major iSMCs. Methods Results— Using combination immunochemical reverse transcription–polymerase chain...
Objective— Transforming growth factor-beta (TGF-β) has been implicated in the pathogenesis of human atherosclerosis but its actions during lesion progression are poorly understood. Smad2, Smad3, and Smad4 proteins signaling molecules by which TGF-β modulates gene transcription. Our objective was to define humans examining expression Smads relation TGF-β–mediated responses. Methods Results— Immunohistochemistry reverse-transcription polymerase chain reaction demonstrated macrophages...
Low density lipoprotein (LDL) from patients with coronary atherosclerosis and diabetes mellitus as well in vitro desialylated LDL, glycosylated (a) caused a twofold to fourfold rise cholesteryl ester cultured human blood monocytes intimal smooth muscle cells isolated normal aorta. Native LDL healthy subjects failed induce intracellular lipid accumulation. We have demonstrated by laser correlative photometry gel filtration chromatography that vivo modified lipoproteins form aggregates under...
The [PSI+] prion determinant of Saccharomyces cerevisiae causes nonsense suppressor phenotype due to a reduced function the translation termination factor Sup35 (eRF3) polymerized into amyloid fibrils. Prion state Rnq1 protein, [PIN+], is required for de novo generation but not propagation. Yeast [psi-] [PIN+] cells overproducing can exhibit suppression without stable [PSI+]. Here, we show that in such cells, most represents polymers, although remaining monomer sufficient normal termination....
The blood serum of patients with coronary atherosclerosis possesses an ability to induce the accumulation cellular lipids in primary cultures human aortic intimal cells. Factors responsible for this property atherosclerotic patients' sera are represented by modified (desialylated) low density lipoprotein (LDL) and a nonlipid factor interacting LDL. It was assumed that antibodies against Total immunoglobulin G (IgG) fraction isolated from patients, IgGs LDL (anti-LDL) were then purified...
The SUP45 and SUP35 genes of Saccharomyces cerevisiae encode polypeptide chain release factors eRF1 eRF3, respectively. It has been suggested that the Sup35 protein (Sup35p) is subject to a heritable conformational switch, similar mammalian prions, thus giving rise non-Mendelian [PSI+] nonsense suppressor determinant. In state, Sup35p forms high-molecular-weight aggregates which may inhibit activity, leading phenotype. composed N-terminal domain (N) required for maintenance, presumably...
The Sup35 (eRF3) translation termination factor of Saccharomyces cerevisiae can undergo a prion-like conformational conversion, thus resulting in the [PSI +] nonsense-suppressor determinant.In vivo this process depends critically on chaperone Hsp104, whose lack or overexpression cure +]. use artificial prion +PS] based hybrid Sup35PS with domain from yeast Pichia methanolicaallowed us to uncover three more chaperones, Ssb1, Ssa1, and Ydj1, determinants. Here, we used search multicopy genomic...
Low density lipoproteins (LDL) isolated from the plasma of patients with angiographically demonstrable coronary heart disease (CHD) induced accumulation triglycerides, free cholesterol, and cholesteryl esters in cultured macrophages, smooth muscle cells, endothelial cells derived uninvolved intima human aorta, but not skin fibroblasts or hepatoma cells.The sialic acid content LDL CHD was 40-75% lower than that healthy donors.There a negative correlation between LDL-induced total...