A5075152395- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Tissue Engineering and Regenerative Medicine
- Ear Surgery and Otitis Media
- Mesenchymal stem cell research
- Hormonal Regulation and Hypertension
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Cardiac Ischemia and Reperfusion
- Corneal Surgery and Treatments
- Cardiac Fibrosis and Remodeling
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Cell Adhesion Molecules Research
- Wnt/β-catenin signaling in development and cancer
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Coronary Interventions and Diagnostics
- Cardiac Structural Anomalies and Repair
- Peroxisome Proliferator-Activated Receptors
- Diabetes Treatment and Management
- Protease and Inhibitor Mechanisms
- Ear and Head Tumors
- Metabolism, Diabetes, and Cancer
- Hearing, Cochlea, Tinnitus, Genetics
The University of Western Australia
2015-2024
Harry Perkins Institute of Medical Research
2022-2024
Queen Elizabeth II Medical Centre
2017-2024
Ear Science Institute Australia
2012-2021
O'Brien Institute
2007-2018
The University of Melbourne
2005-2018
St Vincent's Hospital
2005-2014
St. Vincent's Birmingham
2012
St. Vincent's Hospital
2012
Saint Vincent's Catholic Medical Center
2012
Uninephrectomized rats drinking 1% sodium chloride were given aldosterone (Aldo, 0.75 microgram/h, subcutaneous [s.c.] infusion), deoxycorticosterone (DOC, 20 mg/wk, s.c.), corticosterone (B, 2 mg/d, or the antiglucocorticoid-antiprogestin RU486 (2 s.c.) for 8 wk, and hemodynamic tissue responses compared with a non-steroid-treated control group. Aldo DOC markedly increased systolic BP caused considerable (40-50%) cardiac hypertrophy; B neither hypertension nor hypertrophy. Measurements of...
Human adult mesenchymal stem cells (MSCs) support the engineering of functional tissue constructs by secreting angiogenic and cytoprotective factors, which act in a paracrine fashion to influence cell survival vascularization. MSCs have been isolated from many different sources, but little is known about how factor secretion varies between MSC populations. We evaluated expression patterns adipose (ASCs), bone marrow (BMSCs), dermal tissues [dermal sheath (DSCs) papilla (DPCs)]. Specifically,...
Background —The detrimental effects of high dietary salt intake may not only involve on blood pressure and organ hypertrophy but also lead to tissue fibrosis independently these factors. Methods Results effect a normal (1%) or (8%) sodium chloride diet myocardial renal was assessed by quantitative histomorphometry in spontaneously hypertensive rats (SHRs) normotensive Wistar-Kyoto (WKYs). The transforming growth factor-β 1 (TGF-β ) gene expression Northern blot hybridization. A high-salt...
Background — Atherosclerosis is a major complication of diabetes, but the mechanisms by which diabetes promotes macrovascular disease have not been fully delineated. Although several animal studies demonstrated that inhibition ACE results in decrease development atherosclerotic lesions, information about potential benefits these agents on complex and advanced lesions as observed long-term lacking. The aim this study was to evaluate whether treatment with inhibitor perindopril affects...
Background— Cardiac tissue engineering offers the prospect of a novel treatment for acquired or congenital heart defects. We have created vascularized pieces beating cardiac muscle in rat that are as thick adult right ventricle wall. Method and Results— Neonatal cardiomyocytes Matrigel were implanted with an arteriovenous blood vessel loop into 0.5-mL patented tissue-engineering chamber, located subcutaneously groin. Chambers harvested 1, 4, 10 weeks after insertion. At 4 weeks, all...
Human adipose-derived stem cells (ASCs) may differentiate into cardiomyocytes and this provides a source of donor for tissue engineering. In study, we evaluated cardiomyogenic differentiation protocols using DNA demethylating agent 5-azacytidine (5-aza), modified medium (MCM), histone deacetylase inhibitor trichostatin A (TSA) co-culture with neonatal rat cardiomyocytes. 5-aza treatment reduced both cardiac actin TropT mRNA expression. Incubation in MCM only slightly increased gene...
Large artery stiffening increases cardiovascular risk and promotes isolated systolic hypertension which is more prevalent in elderly women than men. Variation sex steroid levels between males females throughout life may modulate arterial stiffness. We hypothesized that steroids directly influence expression of important structural proteins determine biomechanical properties. Human aortic smooth muscle cells were incubated with physiological concentrations 17beta-estradiol, progesterone,...
To grow more robust cardiac tissue for implantation in vivo, strategies to improve survival of implanted stem cells are required. Here we report the protective effects hypoxic preconditioning (HPC) and identify mechanisms improving adipose-derived (ASC) vitro. Human ASC were preconditioned 24 h with hypoxia then exposed simulated ischemia a further h. HPC significantly increased viability, reduced cell injury apoptosis compared non-preconditioned under ischemic conditions, as shown by...
Adult mesenchymal stem cells secrete a variety of angiogenic cytokines and growth factors, so we proposed that these paracrine mechanisms may be used to promote vascularization for tissue engineering in vivo. We tested whether or not human adipose-derived (ASCs) formation rats. ASCs were evaluated vitro mRNA expression including the vascular endothelial factor, basic fibroblast interleukin-8 (IL-8), stromal cell-derived factor-1 (SDF-1) proliferative activity on microvascular cells. For vivo...
Human adipose-derived stem cells (ASCs) secrete cytokines and growth factors that can be harnessed in a paracrine fashion for promotion of angiogenesis, cell survival, activation endogenous cells. We recently showed hypoxia is powerful stimulus an angiogenic activity from ASCs vitro here we investigate the biological significance this vivo angiogenesis model. A single exposure to severe (<0.1% O2) significantly increased both transcriptional translational level vascular endothelial factor-A...
Hydrogel bioprinting is a major area of focus in the field tissue engineering. However, 3D printed hydrogel scaffolds often suffer from low printing accuracy and poor mechanical properties because their soft nature tendency to shrink. This makes it challenging process them into structural materials. In this study, natural chitosan were, for first time, reinforced with milled silk particles fabricated by printing. Compared pure scaffolds, addition resulted up 5-fold increase compressive...
Some animal studies suggest that transforming growth factor-beta (TGF-beta) protects vessels from atherosclerosis by preventing intima formation, but others indicate a role in vessel proteoglycan accumulation and lipoprotein retention. To distinguish between these possibilities humans, immunohistochemical were performed examining the coexpression of TGF-beta isoforms receptors ALK-5 TbetaR-II aorta during various stages atherosclerotic lesion development.The spatial relationships TGF-beta1,...
Background —β-Adrenergic signaling is downregulated in the failing heart, and significance of such change remains unclear. Methods Results —To address role β-adrenergic dysfunction heart failure (HF), aortic stenosis (AS) was induced wild-type (WT) transgenic (TG) mice with cardiac targeted overexpression β 2 -adrenergic receptors (ARs), animals were studied 9 weeks later. The extents increase systolic arterial pressure ( P <0.01 versus controls), left ventricular (LV) hypertrophy (TG,...
Tympanic membrane (TM) perforations lead to significant hearing loss and result in possible infection of the middle ear. Myringoplasty is commonly performed repair chronic perforations. Although various grafts materials have been used promote TM regeneration, all associated limitations. The aim this study was evaluate efficacy feasibility two graft materials, silk fibroin scaffold (SFS) porcine-derived acellular collagen type I/III (ACS), compared with (paper patch Gelfoam) for promotion...
Androgens may contribute to higher cardiovascular risk in men via deleterious effects on vascular endothelial cells (EC). We examined the of androgens male human umbilical vein EC (EA.hy926) culture. [3H]Thymidine incorporation assays showed that after 24-h serum deprivation, testosterone (T) (but not dehydroepiandrosterone nor 17β-E2) induced significant dose-dependent decreases DNA synthesis (10–16% at 1–100 nmol/liter); AR antagonist flutamide (100 nmol/liter) abolished this effect T....