A5088303936- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Adipokines, Inflammation, and Metabolic Diseases
- Adipose Tissue and Metabolism
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Atherosclerosis and Cardiovascular Diseases
- Electrospun Nanofibers in Biomedical Applications
- Wnt/β-catenin signaling in development and cancer
- Cardiovascular Disease and Adiposity
- Kruppel-like factors research
- TGF-β signaling in diseases
- Cancer-related molecular mechanisms research
- Nerve injury and regeneration
- Cell Adhesion Molecules Research
- Periodontal Regeneration and Treatments
- Cancer-related Molecular Pathways
- Pluripotent Stem Cells Research
- Nitric Oxide and Endothelin Effects
- Biomarkers in Disease Mechanisms
- COVID-19 Clinical Research Studies
- RNA Interference and Gene Delivery
- Virus-based gene therapy research
- Protease and Inhibitor Mechanisms
Lomonosov Moscow State University
2016-2025
Moscow State University
2009-2025
Institute of Surgery named after A. V. Vishnevskogo
2012
Institute of Experimental Cardiology
1999-2008
The Alfred Hospital
2001-2004
Ministry of Health of the Russian Federation
2000-2002
Montana State University
2002
Transplantation of adipose-derived mesenchymal stem cells (ASCs) induces tissue regeneration by accelerating the growth blood vessels and nerve. However, mechanisms which they accelerate nerve fibers are only partially understood. We used transplantation ASCs with subcutaneous matrigel implants (well-known in vivo model angiogenesis) mice limb reinnervation to check influence ASC on growth. Here we show that stimulate nerves innervated mice's limbs induce axon implants. To investigate...
Several studies demonstrate the role of adipose mesenchymal stem cells (ASCs) in angiogenesis. The angiogenic mechanism has been ascribed to paracrine factors since these secrete a plenty signal molecules and growth factors. Recently it suggested that besides soluble factors, extracellular vesicles (EVs) include exosomes microvesicles may play major cell-to-cell communication. It shown EVs are implicated process. Herein we studied whether released by ASCs mediate activity cells. Our results...
Atherosclerosis is a chronic inflammatory response of the arterial wall to injury. High-mobility group box 1 (HMGB1) DNA-binding protein, which on release from cells exhibits potent actions. We examined its expression in atherosclerotic lesions and regulation by cytokines.In lesions, HMGB1 protein expressed endothelial cells, some intimal smooth muscle macrophages. As atherosclerosis develops progresses fatty streaks fibrofatty lesion, number HMGB1-producing macrophages increases markedly....
Abstract Background Mesenchymal stem cells derived from adipose tissue (ADSC) are multipotent cells, originated the vascular-stromal compartment of fat tissue. ADSC used as an alternative cell source for many different therapies, however in ischemic cardiovascular diseases therapeutic benefit was modest. One reasons could be use autologous aged ADSC, which recently were found to have impaired functions. We therefore analysed effects age on markers and angiogenic properties ADSC. Hypoxic...
Adipose-derived stromal cells (ASCs) are suggested to be potent candidates for cell therapy of ischemic conditions due their ability stimulate blood vessel growth. ASCs produce many angiogenic and anti-apoptotic growth factors, secretion is significantly enhanced by hypoxia. Utilizing a Matrigel implant model, we showed that hypoxia-treated stimulated angiogenesis as well maturation the newly formed vessels in vivo. To elucidate mechanisms ASC action, used co-culture model with isolated from...
Abstract Introduction This study was aimed at deciphering the secretome of adipose-derived mesenchymal stromal cells (ADSCs) cultured in standard and hypoxic conditions to reveal proteins, which may be responsible for regenerative action these cells. Methods Human ADSCs were isolated from 10 healthy donors 3–4 passages. Cells serum deprived cell purity assessed using multiple surface markers. Conditioned media collected analyzed LC-MS with a focus on characterizing secreted proteins. Results...
Mesenchymal stem/stromal cells (MSC) remain a promising tool for regenerative medicine as the efficacy of MSC-based cell therapy has been demonstrated broad spectrum indications. Their therapeutic potency is mainly associated with their ability to secrete multiple factors critical tissue regeneration. Due comparable effects along superior safety MSC conditioned medium (MSC-CM) containing complex MSC-secreted products considered reasonable alternative therapy. However, lack standards...
Tissue regeneration is impaired in aged individuals. Adipose-derived mesenchymal stromal cells (ADSCs), a promising source for cell therapy, were shown to secrete various angiogenic factors and improve vascularization of ischemic tissues. We analyzed how patient age affected the properties ADSCs. ADSCs isolated from subcutaneous fat tissue patients with coronary artery disease (CAD; n = 64, 43-77 years old) without CAD (n 31, 2-82 old). ADSC phenotype characterized by flow cytometry was...
It has been established in the recent several decades that stem cells play a crucial role tissue renewal and regeneration. Mesenchymal (MSCs) are part of most important population adult cells. These have hereby identified for very first time subsequently isolated from bone marrow stroma. Bone marrow-derived MSCs believed to source repair connective tissues, including bone, cartilage adipose tissues. Cells similar now all postnatal Data on distribution function MSCsin vivocollected using...
Multipotent mesenchymal stem/stromal cells (MSC) including adipose-derived stromal (ADSC) have been successfully applied for cardiovascular diseases treatment. Their regenerative potential is considered due to the multipotency, paracrine activity and immunologic privilege. However, therapeutic efficacy of autologous MSC myocardial ischemia therapy modest. We analyzed if ADSC properties are attenuated in patients with chronic such as coronary artery disease (CAD) diabetes mellitus type 2...
Fibroblasts differentiation into myofibroblasts is a central event of tissue fibrosis. Multipotent mesenchymal stromal cells (MSCs) secretome can interfere with fibrosis development; despite precise underlying mechanisms remain unclear. In this study, we tested the hypothesis that MSC affect fibroblast’ by delivering regulatory RNAs, including microRNAs to these cells. Using model transforming growth factor-beta (TGFbeta)-induced fibroblast myofibroblasts, activity human components,...
Objective— Despite studies implicating superoxide anion–producing oxidases in atherosclerosis, their characteristics, expression, and regulation cells of lesions are poorly understood. We examined the following: (1) whether cytochrome b 558 –dependent NAD(P)H oxidase–phox peptides expressed by intimal smooth muscle (iSMCs) macrophages human aortic atherosclerotic (2) oxidase represents a major iSMCs. Methods Results— Using combination immunochemical reverse transcription–polymerase chain...
Objective— Transforming growth factor-beta (TGF-β) has been implicated in the pathogenesis of human atherosclerosis but its actions during lesion progression are poorly understood. Smad2, Smad3, and Smad4 proteins signaling molecules by which TGF-β modulates gene transcription. Our objective was to define humans examining expression Smads relation TGF-β–mediated responses. Methods Results— Immunohistochemistry reverse-transcription polymerase chain reaction demonstrated macrophages...
The study of molecular mechanisms regeneration requires convenient models for in vitro and vivo studies. In cell cultures can fulfill such a function. However, their rapid aging loss initial specific properties culture is significant limitation use. Telomerase expression help to overcome these limitations: it prolong proliferative activity stabilize the primary culture. Here, we created studied human adipose tissue multipotent mesenchymal stromal (MSC) that overexpress catalytic protein...
Abstract Sympathetic neurons are important components of mesenchymal stem cells (MSCs) niche and noradrenaline regulates biological activities these cells. Here we examined the mechanisms regulation MSCs responsiveness to noradrenaline. Using flow cytometry, demonstrated that α1A adrenergic receptors isoform was most abundant in adipose tissue-derived MSCs. calcium imaging single cells, only 6.9 ± 0.8% responded by intracellular release. Noradrenaline increases sensitivity catecholamines a...
Obesity is often associated with high systemic and local activity of renin-angiotensin system (RAS). Mesenchymal stem cells adipose tissue are the main source adipocytes. The aim this study was to clarify how RAS could control differentiation human derived mesenchymal (ADSCs). We examined distribution angiotensin receptor expressing in found that type 1 2 receptors co-expressed its stromal compartment, which known contain cells. To expression specifically ADSCs we have isolated them from...
The effect of substances known as inducers neuronal differentiation on cultured human and mouse adipose-derived mesenchymal stem cells (ASCs) their fate after transplantation into the injured ischemic brains were studied. ASCs isolated from adipose tissue. Inducers included β-mercaptoethanol, glial cell line-derived neurotrophic factor (GNDF), brain-derived (BDNF), retinoic acid (RA), 5-azacytidine, well combinations. Three days induction, phenotype induced was analyzed using...