A5069826761- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Angiogenesis and VEGF in Cancer
- Protease and Inhibitor Mechanisms
- Virus-based gene therapy research
- Cell Adhesion Molecules Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurological Disorders and Treatments
- Acute Ischemic Stroke Management
- Extracellular vesicles in disease
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Thermoregulation and physiological responses
- 3D Printing in Biomedical Research
- Cardiac and Coronary Surgery Techniques
- Healthcare Systems and Public Health
- Nutrition, Genetics, and Disease
- Optical Imaging and Spectroscopy Techniques
- Apelin-related biomedical research
- Barrier Structure and Function Studies
- TGF-β signaling in diseases
- Cardiovascular Disease and Adiposity
- Viral Infectious Diseases and Gene Expression in Insects
Institute of Experimental Cardiology
2015-2025
V.A. Negovsky Scientific Research Institute of General Reanimatology
2018-2025
National Medical Research Center of Cardiology
2017-2025
Ministry of Health of the Russian Federation
2016-2023
CARE USA
2022
Lomonosov Moscow State University
2015-2018
Adipose-derived stromal cells (ASCs) are suggested to be potent candidates for cell therapy of ischemic conditions due their ability stimulate blood vessel growth. ASCs produce many angiogenic and anti-apoptotic growth factors, secretion is significantly enhanced by hypoxia. Utilizing a Matrigel implant model, we showed that hypoxia-treated stimulated angiogenesis as well maturation the newly formed vessels in vivo. To elucidate mechanisms ASC action, used co-culture model with isolated from...
Modified cell-based angiogenic therapy has become a promising novel strategy for ischemic heart and limb diseases. Most studies focused on myoblast, endothelial cell progenitors or bone marrow mesenchymal stromal cells transplantation. Yet adipose-derived (in contrast to marrow) are abundantly available can be easily harvested during surgery liposuction. Due high paracrine activity availability ADSCs appear preferable type cardiovascular therapy. Still neither genetic modification of human...
This is the first in vivo study to investigate neuroprotective effects of krypton on focal cerebral ischemia. The aim was analyze effect 2 h inhalation a krypton–oxygen mixture (Kr 70%/O2 30%) recovery neurological functions and degree brain damage rats after photoinduced ischemic stroke (PIS) possible mechanisms responsible for this neuroprotection. Experiments were performed male Wistar weighing 250–300 g (n = 32). Animals randomized into four groups. Two groups 20) underwent stroke,...
Aim. To identify personalized morphological neuronal phenotypes based on the distribution pattern of protein NeuN in cerebral cortex layers. Materials and Methods . A histologic study was performed rats (N=10). Tissue sections were stained with hematoxylin eosin, nuclear visualized by immunohistochemical staining. Analysis microscopy image analysis software. Results. staining revealed distinct localization intensity patterns within cortical neurons. Contrary to definition as a protein, its...
The COVID-19 pandemic had an unprecedented impact on all aspects of human activity worldwide, frequently resulting in post-acute sequelae and affecting multiple organ systems. underlying mechanisms driving both acute manifestations are still poorly understood, warranting further investigation for new targets. study represents the first attempt to explore role T-cadherin pathogenesis as well its implications pulmonary fibrosis endothelial dysfunction. First, we revealed a significant decrease...
Increased interest in development of combined gene therapy emerges from results recent clinical trials that indicate good safety yet unexpected low efficacy "single-gene" administration. Multiple studies showed vascular endothelial growth factor 165 aminoacid form (VEGF165) and hepatocyte (HGF) can be used for induction angiogenesis ischemic myocardium skeletal muscle. Gene transfer system composed a novel cytomegalovirus-based (CMV) plasmid vector codon-optimized human VEGF165 HGF genes...
The adult heart contains small populations of multipotent cardiac progenitor cells (CPC) that present a convenient and efficient resource for treatment myocardial infarction. Several clinical studies direct CPC delivery by injection have already been performed but showed low engraftment rate limited beneficial effects procedure. «Cell sheet» technology has developed to facilitate longer retention grafted show new directions cell-based therapy using this strategy. In study we hypothesized...
Since development of plasmid gene therapy for therapeutic angiogenesis by J. Isner this approach was an attractive option ischemic diseases affecting large cohorts patients. However, first placebo-controlled clinical trials showed its limited efficacy questioning further advance to practice. Thus, combined methods using delivery several angiogenic factors got into spotlight as a way improve outcomes. This study provides experimental proof concept simultaneous VEGF165 and HGF genes alleviate...
Pulmonary fibrosis is a common and threatening post-COVID-19 complication with poorly resolved molecular mechanisms no established treatment. The plasminogen activator system, including urokinase (uPA) receptor (uPAR), involved in the pathogenesis of COVID-19 contributes to development lung injury pulmonary fibrosis, although their cellular underpinnings still remain obscure. aim current study was assess role uPA uPAR fibrosis. We analyzed expression human tissues from patients using...
Urokinase plasminogen activator (uPA) is required for both endogenous and vascular endothelial growth factor (VEGF)–augmented angiogenesis in normal tissues, leading us to hypothesize that uPA augmentation by gene transfer might promote ischemic tissues. Overexpression of was studied rat myocardial infarction (MI) mouse hind limb ischemia models compared with VEGF overexpression effects. Animals were divided into control three experimental groups (n = 6), receiving intramuscular injections...
Cell therapy remains a promising approach for the treatment of cardiovascular diseases. In this regard, contemporary trend is development methods to overcome low cell viability and enhance their regenerative potential. present study, we evaluated therapeutic potential gene-modified adipose-derived stromal cells (ADSC) that overexpress hepatocyte growth factor (HGF) in mice hind limb ischemia model. Angiogenic neuroprotective effects were assessed following ADSC transplantation suspension or...
Cell therapy of the post-infarcted myocardium is still far from clinical use. Poor survival transplanted cells, insufficient regeneration, and replacement damaged tissue limit potential currently available cell-based techniques. In this study, we generated a multilayered construct adipose-derived mesenchymal stromal cells (MSCs) modified to secrete stem cell factor, SCF. rat model infarction, show that transplantation SCF producing sheet induced activation epicardium promoted accumulation...
Relevance. The increase in the number of severe brain injuries due to stroke and traumatic injury determines need study develop effective strategies for neuroprotection. article highlights new mechanisms neuroprotective action inhalation anesthetic xenon based on data our own experimental studies. Aim study. To assess effect anesthesia with at a concentration 0.5 MAC (minimum alveolar concentration) phosphorylation glycogen synthase kinase 3β (GSK-3β) content antioxidant defense enzymes rat...
Now, great interest is connected with application of the cells received directly from a myocardium as tool for stimulation angiogenesis and reparative processes in heart. The aim present research studying angiogenic properties vitro vivo c-kit+-cells, obtained hearts small rodents. work was performed on male C57BL/6 mice Wistar rats. study used methods cytofluorimetry, immuno-fluorescent staining, models myocardial infarction subcutaneous Matrigel™. It shown, that after acute c-kit+...