A5067164448- Muscle Physiology and Disorders
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Autophagy in Disease and Therapy
- Viral-associated cancers and disorders
- Ubiquitin and proteasome pathways
- Banana Cultivation and Research
- Congenital heart defects research
- Plant Physiology and Cultivation Studies
- Neuroblastoma Research and Treatments
- Protease and Inhibitor Mechanisms
- Genetics and Neurodevelopmental Disorders
- Innovation and Socioeconomic Development
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cytomegalovirus and herpesvirus research
- DNA and Nucleic Acid Chemistry
- Lymphoma Diagnosis and Treatment
- Cardiomyopathy and Myosin Studies
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tissue Engineering and Regenerative Medicine
Institut Gustave Roussy
2020-2024
Centre National de la Recherche Scientifique
2020-2024
Koltzov Institute of Developmental Biology
2021-2024
Université Paris-Saclay
2021-2024
Université Paris-Sud
2020-2023
Lomonosov Moscow State University
2020-2021
All-Russian Scientific Research Institute of Horticulture named after IV Michurin
2021
Abstract Imbalance in the finely orchestrated system of chromatin-modifying enzymes is a hallmark many pathologies such as cancers, since causing affection epigenome and transcriptional reprogramming. Here, we demonstrate that loss-of-function mutation (LOF) major histone lysine methyltransferase SETDB1 possessing oncogenic activity lung cancer cells leads to broad changes overall architecture mechanical properties nucleus through genome-wide redistribution heterochromatin, which perturbs...
Abstract Burkitt lymphoma (BL) is a B cell malignancy associated with the Epstein−Barr virus (EBV). Most BL cases are characterized by t(8;14) chromosomal translocation involving MYC oncogene and immunoglobulin heavy chain gene ( IGH ). The role of EBV in promoting this remains largely unknown. Here we provide experimental evidence that reactivation from latency leads to an increase proximity between loci, otherwise located far away nuclear space both B‐lymphoblastoid lines patients'...
Pulmonary fibrosis is a common and threatening post-COVID-19 complication with poorly resolved molecular mechanisms no established treatment. The plasminogen activator system, including urokinase (uPA) receptor (uPAR), involved in the pathogenesis of COVID-19 contributes to development lung injury pulmonary fibrosis, although their cellular underpinnings still remain obscure. aim current study was assess role uPA uPAR fibrosis. We analyzed expression human tissues from patients using...
Many muscular pathologies are associated with oxidative stress and elevated levels of the tumor necrosis factor (TNF) that cause muscle protein catabolism impair myogenesis. Myogenesis defects caused by TNF mediated in part reactive oxygen species (ROS), including those produced mitochondria (mitoROS), but mechanism their pathological action is not fully understood. We hypothesized mitoROS act triggering enhancing mitophagy, an important tool for remodelling mitochondrial reticulum during...
A new mitochondria-targeted probe MitoCLox was designed as a starting compound for series of probes sensitive to cardiolipin (CL) peroxidation. Fluorescence microscopy reported selective accumulation in mitochondria diverse living cell cultures and its oxidation under stress conditions, particularly those known cause oxidation. Ratiometric fluorescence measurements using flow cytometry showed remarkable dependence the dynamic range on sample. Specifically, induced by low doses hydrogen...
Muscles of patients with facioscapulohumeral dystrophy (FSHD) are characterized by sporadic DUX4 expression and oxidative stress which is at least partially induced protein. Nevertheless, targeting antioxidants has a limited impact on FSHD patients, the exact role in pathology FSHD, as well its interplay expression, remain unclear. Here we set up screen for genes that upregulated via aim to target these rather than itself. Immortalized human myoblasts expressing (MB135-DUX4) have an...
uPAR is a membrane receptor that binds extracellular protease urokinase, contributes to matrix remodeling and plays crucial role in cellular adhesion, proliferation, survival, migration. overexpression tumor cells promotes mitogenesis, opening prospective avenue for targeted therapy. However, targeting cancer has potential risks. We have recently shown downregulation neuroblastoma epithelial-mesenchymal transition (EMT), potentially associated with metastasis chemoresistance. used data...
The most common form of facioscapulohumeral dystrophy (FSHD1) is caused by a partial loss the D4Z4 macrosatellite repeat array in subtelomeric region chromosome 4. Patients with FSHD1 typically carry 1 to 10 repeats, whereas nonaffected individuals have 11 150 repeats. ~150-kilobyte 10q exhibits ~99% sequence identity 4q, including array. Nevertheless, contractions chr10 do not cause FSHD or any known disease, as people on flanked nonfunctional polyadenylation signal, permitting DUX4...
Abstract Discovery of the CRISPR/Cas system revolutionized biology and biomedicine in 21st century. Here we discuss milestones development genome editing technology, from history discovery to current developments, including medical applications. Technical ethical problems associated with use for human embryonic genomes are also discussed.
Abstract Most cancer-related chromosomal translocations appear to be cell type specific. It is currently unknown why different occur in cells. This can due either the occurrence of particular specific types or adaptive survival advantage conferred by only We experimentally addressed this question double-strand break (DSB) induction at MYC, IGH, AML and ETO loci same generate lineages. Our results show that any translocation potentially arise type. have analyzed factors could affect frequency...
Abstract Facioscapulohumeral muscular dystrophy (FSHD) is a genetic disease associated with ectopic expression of the DUX4 gene in skeletal muscle. Muscle degeneration FSHD accompanied by muscle tissue replacement fat and connective tissue. Expression myoblasts stimulates mesenchymal stem cells (MSC) migration via CXCR4‐CXCL12 axis. MSCs participate adipose formation can contribute to fibrosis. Here we studied interaction between consequences this context. We used cell motility assays...
Abstract Imbalance in the finely orchestrated system of chromatin-modifying enzymes is a hallmark many pathologies such as cancers, since causing affection epigenome and transcriptional reprogramming. Here, we demonstrate that loss-of-function mutation (LOF) major histone lysine methyltransferase SETDB1 possessing oncogenic activity lung cancer cells leads to broad changes overall architecture mechanical properties nucleus through genome-wide redistribution heterochromatin, which perturbs...