A5089741610- Protease and Inhibitor Mechanisms
- Signaling Pathways in Disease
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Nerve injury and regeneration
- Peptidase Inhibition and Analysis
- Epigenetics and DNA Methylation
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- S100 Proteins and Annexins
- Neurogenesis and neuroplasticity mechanisms
- 14-3-3 protein interactions
- CRISPR and Genetic Engineering
- Innovation and Socioeconomic Development
- Renal and related cancers
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Digital Transformation in Law
- Phagocytosis and Immune Regulation
- Cancer Genomics and Diagnostics
- Nuclear Receptors and Signaling
- Genetics and Neurodevelopmental Disorders
- Neuroblastoma Research and Treatments
- Virus-based gene therapy research
École Polytechnique
2022-2025
Centre National de la Recherche Scientifique
2024-2025
Institute of Experimental Cardiology
2015-2022
Lomonosov Moscow State University
2016-2022
National Medical Research Center of Cardiology
2019-2022
Ministry of Health of the Russian Federation
2015-2021
Recent findings indicate the significant contribution of urokinase and receptor (uPA uPAR) in processes nerve regeneration, however, their role axonal growth branching is unclear. Using a 3D model mouse Dorsal Root Ganglia (DRG) explants, differentiated into neurons Neuro 2a cells transgenic mice lacking gene, we studied involvement uPA/uPAR system neural cell migration, neurite outgrowth, elongation branching.uPA uPAR are expressed cones axons. an ex vivo DRG explants Matrigel have found...
Abstract The urokinase system is involved in a variety of physiological processes, such as fibrinolysis, matrix remodeling, wound healing, and regeneration. Upon binding to its cognate receptor urokinase‐type plasminogen activator (uPAR), (uPA) catalyzes the conversion plasmin activation metalloproteases. Apart from this, uPA–uPAR interaction can lead transcription factors, mitogen‐activated protein kinase signaling pathways RTK cascades. Elevated expression uPA uPAR markedly associated with...
// Karina D. Rysenkova 1 , Ekaterina V. Semina 1, 2 Maxim N. Karagyaur 3 Anna A. Shmakova Daniyar T. Dyikanov Petr Vasiluev Yury P. Rubtsov 4 Kseniya Rubina and Vsevolod Tkachuk Lomonosov Moscow State University, Faculty of Medicine, Laboratory Gene Cell Technologies, 119991, Moscow, Russian Federation Federal Budgetary Organization National Cardiology Research Center Ministry Health the Federation, Institute Experimental Cardiology, 121552, Regenerative Shemyakin-Ovchinnikov Bioorganic...
Urokinase-type plasminogen activator uPA and its receptor (uPAR) are the central players in extracellular matrix proteolysis, which facilitates cancer invasion metastasis. EGFR is one of important components uPAR interactome. uPAR/EGFR interaction controls signaling pathways that regulate cell survival, proliferation migration. We have previously established binding to stimulates neurite elongation neuroblastoma cells, while blocking uPA/uPAR induces branching new formation. Here we...
Urokinase receptor (uPAR) promotes extracellular matrix proteolysis, regulates adhesion and cell migration, transduces intracellular signals through interactions with the lateral partners. The expression of uPAR urokinase (uPA) is significantly upregulated in peripheral nerves after injury, however, little known about function nerve regeneration or molecular mechanisms involved. purpose this study to investigate role traumatic injury n. Peroneus communis uPA-/-, uPAR-/- control mice (WT)...
uPAR is a membrane receptor that binds extracellular protease urokinase, contributes to matrix remodeling and plays crucial role in cellular adhesion, proliferation, survival, migration. overexpression tumor cells promotes mitogenesis, opening prospective avenue for targeted therapy. However, targeting cancer has potential risks. We have recently shown downregulation neuroblastoma epithelial-mesenchymal transition (EMT), potentially associated with metastasis chemoresistance. used data...
Urokinase receptor (uPAR) is a glycosylphosphatidylinositol (GPI)-anchored of urokinase (uPA), which involved in brain development, nerve regeneration, wound healing and tissue remodeling. We have recently shown that Plaur, encodes uPAR, an early response gene murine brain. Assumingly, diverse functions Plaur might be attributed to hypothetical, unidentified microRNAs encoded within introns the gene. Using bioinformatic approach we identified novel small RNAs named them Plaur-miR1-3p...
Most commonly used cell lines are readily susceptible to genome editing and present a good object for models establish disease-causing genes find ways cure diseases. However, karyotype phenotype heterogeneity between individual cells in such cultures as well multiplicity of target alleles make generation desired by single-cell cloning (used diploid cells) inapplicable. We designed tested simple approach targeted modification single sizable populations, containing multiple variants. To obtain...
Abstract Epileptogenesis progressively leads to the rearrangement of normal neuronal networks into more excitable ones and can be viewed as a form neuroplasticity, molecular mechanisms which still remain obscure. Here, we studied pentylenetetrazole seizure‐induced regulation genes for plasminogen activator system in mouse brain. We found that expression tissue (tPA) urokinase receptor (uPAR) mRNA was strongly increased cerebral cortex, hippocampus, striatum amygdala early 3 hr after...
Whole exome sequencing of invasive mammary carcinomas revealed the association mutations in PTEN and ZFHX3 tumor suppressor genes (TSGs). We generated single combined knock-outs (KOs) immortalized epithelial cell line MCF10A to study role these their potential synergy migration regulation. Inactivation PTEN, but not ZFHX3, induced formation large colonies soft agar. inactivation KO, however, increased colony numbers normalized size. Cell was affected different ways upon KO. enhanced...
Breast cancer develops upon sequential acquisition of driver mutations in mammary epithelial cells; however how these collaborate to transform normal cells remains unclear most cases. We aimed reconstitute this process a particular case. To end, we combined the activated form PI 3-kinase harboring H1047R mutation with inactivation histone lysine methyl-transferase KMT2D non-tumorigenic human cell line MCF10A. found that activation promoted cycle progression, especially when growth signals...
Breast cancer develops upon sequential acquisition of driver mutations in mammary epithelial cells; however, how these collaborate to transform normal cells remains unclear most cases. We aimed reconstitute this process a particular case. To end, we combined the activated form PI 3-kinase harboring H1047R mutation with inactivation histone lysine methyl-transferase KMT2D non-tumorigenic human cell line MCF10A. found that activation promoted cell-cycle progression, especially when growth...