A5028843398- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- Pluripotent Stem Cells Research
- Adipokines, Inflammation, and Metabolic Diseases
- Pancreatic function and diabetes
- Reproductive System and Pregnancy
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Metabolism, Diabetes, and Cancer
- Cancer Cells and Metastasis
- Free Radicals and Antioxidants
- Biochemical effects in animals
- Endometriosis Research and Treatment
- FOXO transcription factor regulation
- Muscle Physiology and Disorders
- Cell Image Analysis Techniques
- Bone health and treatments
- Single-cell and spatial transcriptomics
- Cardiovascular Disease and Adiposity
- Uterine Myomas and Treatments
- CRISPR and Genetic Engineering
- Fibroblast Growth Factor Research
- melanin and skin pigmentation
- Digital Imaging for Blood Diseases
- Parathyroid Disorders and Treatments
Lomonosov Moscow State University
2012-2025
Moscow State University
2019-2025
Research Center of Neurology
2012
Extracellular matrix (ECM) provides both structural support and dynamic microenvironment for cells regulating their behavior fate. As a critical component of stem cell niche ECM maintains activates proliferation differentiation under specific stimuli. Mesenchymal stem/stromal (MSCs) regulate tissue-specific functions locating in immediate producing various bioactive factors, including components. We evaluated the ability MSC-produced to restore progenitor vitro analyzed possible mechanisms...
Fibroblasts differentiation into myofibroblasts is a central event of tissue fibrosis. Multipotent mesenchymal stromal cells (MSCs) secretome can interfere with fibrosis development; despite precise underlying mechanisms remain unclear. In this study, we tested the hypothesis that MSC affect fibroblast’ by delivering regulatory RNAs, including microRNAs to these cells. Using model transforming growth factor-beta (TGFbeta)-induced fibroblast myofibroblasts, activity human components,...
The study of molecular mechanisms regeneration requires convenient models for in vitro and vivo studies. In cell cultures can fulfill such a function. However, their rapid aging loss initial specific properties culture is significant limitation use. Telomerase expression help to overcome these limitations: it prolong proliferative activity stabilize the primary culture. Here, we created studied human adipose tissue multipotent mesenchymal stromal (MSC) that overexpress catalytic protein...
Multipotent mesenchymal stromal cells (MSCs) integrate hormone and neuromediator signaling to coordinate tissue homeostasis, renewal regeneration. To facilitate the investigation of MSC biology, stable immortalized cell lines are created (e.g., commercially available ASC52telo). However, ASC52telo line has an impaired adipogenic ability a depressed response hormones, including 5-HT, GABA, glutamate, noradrenaline, PTH insulin compared primary cells. This markedly reduces potential in...
Mesenchymal stromal cells (MSCs) are the key regulators of tissue homeostasis and repair after damage. Accumulating evidence indicates dual contribution MSCs into development fibrosis induced by chronic injury: these can suppress fibrotic process due to paracrine activity, but their promoting role in differentiating myofibroblasts has also been demonstrated. Many model systems reproducing have shown ability peroxisome proliferator-activated receptor (PPAR) agonists reverse myofibroblast...
Multipotent stromal cells (MSC) demonstrate remarkable functional heterogeneity; however, its molecular mechanisms remain largely obscure. In this study, we explored MSC response to hormones, which activate Gs-protein / cyclic AMP (cAMP) protein kinase A (PKA) dependent signaling, at the single cell level using genetically encoded biosensor PKA-Spark. For first time, demonstrated that about half of cultured MSCs are not able cAMP/PKA pathway, possibly due limited availability adenylyl...
Multipotent mesenchymal stromal cells (MSCs) maintain cellular homeostasis and regulate tissue renewal repair both by differentiating into mesodermal lineage, e.g., adipocytes, or managing the functions of differentiated cells. Insulin is a key physiological inducer MSC differentiation disturbances in insulin sensitivity could negatively affect adipose renewal. During aging, regulation may be disrupted due to altered signaling potential senescent MSCs, promoting development serious metabolic...
A primary culture of murine cerebellar neurons was used to induce oxidative stress resulting in the accumulation reactive oxygen species (ROS) and activation ERK 1/2 kinase. Short-term incubation (15 min) with homocysteine (HC) or N-methyl-D-aspartate (NMDA) induced partial phosphorylation thus providing enzyme. Inhibitors NMDA receptors, MK-801 D-AP5, both prevented cells by HC NMDA. Another receptor-dependent means stimulation is exposure cardiac glycoside ouabain, a specific inhibitor...
Modern biomedical science still experiences a significant need for easy and reliable sources of human cells. They are used to investigate pathological processes underlying disease, conduct pharmacological studies, eventually applied as therapeutic product in regenerative medicine. For decades, the pool adult mesenchymal stem/stromal cells (MSCs) remains promising source stem progenitor Their isolation is more feasible than most other from donors, yet they have fair share drawbacks. include...
Hypertension is a major risk factor for cardiovascular diseases, such as strokes and myocardial infarctions. Nearly 70% of hypertension onsets in adults can be attributed to obesity, primarily due sympathetic overdrive the dysregulated renin-angiotensin system. Sympathetic increases vasoconstriction via α1-adrenoceptor activation on vascular cells. Despite fact that outflow individuals with rule, there cohort patients obesity who do not develop hypertension. In this study, we investigated...
T-cadherin, a non-canonical member of the cadherin superfamily, was initially identified for its involvement in homophilic recognition within nervous and vascular systems. Apart from adhesive function, T-cadherin acts as receptor two ligands: LDL, contributing to atherogenic processes, HMW adiponectin, hormone with well-known cardiovascular protective properties. However, precise role adipose tissue remains elusive. Previously, we generated Cdh13
Multipotent mesenchymal stromal cells (MSCs) regulate tissue repair through paracrine activity, with secreted proteins being significant contributors. Human commonly results in fibrosis, where fibroblast differentiation into myofibroblasts is a major cellular mechanism. MSCs’ activity can inhibit fibrosis development. We previously demonstrated that the separation of MSC secretome, represented by conditioned medium (CM), subfractions enriched extracellular vesicles (EV) or soluble factors...
Most commonly used cell lines are readily susceptible to genome editing and present a good object for models establish disease-causing genes find ways cure diseases. However, karyotype phenotype heterogeneity between individual cells in such cultures as well multiplicity of target alleles make generation desired by single-cell cloning (used diploid cells) inapplicable. We designed tested simple approach targeted modification single sizable populations, containing multiple variants. To obtain...
Parathyroid hormone (PTH) is one of the key regulators calcium and phosphate metabolism in body, controlling bone ion excretion by kidneys. At present, attempts to use PTH as a therapeutic agent have been associated with side-effects, nature which not always clear predictable. In addition, it known that vivo impairment post-receptor signaling atypical differentiation behavior only cells, but also connective tissues, including adipose tissue. this work, we studied functional responses...