A5065067811- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Reconstructive Surgery and Microvascular Techniques
- Electrospun Nanofibers in Biomedical Applications
- Cancer Cells and Metastasis
- Wound Healing and Treatments
- Hippo pathway signaling and YAP/TAZ
- Reconstructive Facial Surgery Techniques
- CAR-T cell therapy research
- Endometrial and Cervical Cancer Treatments
- Connective tissue disorders research
- Zebrafish Biomedical Research Applications
- Medical and Biological Sciences
- Arctic and Russian Policy Studies
- Angiogenesis and VEGF in Cancer
- Economic and Technological Developments in Russia
- RNA Interference and Gene Delivery
- Human Health and Disease
- Congenital limb and hand anomalies
- Hydrogen's biological and therapeutic effects
- Digestive system and related health
- Estrogen and related hormone effects
Lomonosov Moscow State University
2018-2024
Moscow State University
2024
Extracellular matrix (ECM) provides both structural support and dynamic microenvironment for cells regulating their behavior fate. As a critical component of stem cell niche ECM maintains activates proliferation differentiation under specific stimuli. Mesenchymal stem/stromal (MSCs) regulate tissue-specific functions locating in immediate producing various bioactive factors, including components. We evaluated the ability MSC-produced to restore progenitor vitro analyzed possible mechanisms...
Besides certain exceptions, healing of most tissues in the human body occurs via formation scar tissue, rather than restoration lost structures. After extensive acute injuries, this phenomenon substantially limits possibility function recovery and, case chronic injury, it leads to pathological remodeling organs affected. Managing outcomes damaged tissue repair is one main objectives regenerative medicine. The first priority for reaching comparative investigation mechanisms responsible...
We report a comparative study of multipotent mesenchymal stromal cells (MSC) delivered by injection, MSC-based cell sheets (CS) or MSC secretome to induce healing cutaneous pressure ulcer in C57Bl/6 mice. found that transplantation CS from adipose-derived resulted reduction fibrosis and recovery skin structure with its appendages (hair glands). Despite short retention on surface (3–7 days) it induced profound changes granulation tissue (GT) structure, increasing thickness altering...
Regeneration is a fundamental process much attributed to functions of adult stem cells. In last decades delivery suspended cells widely adopted in regenerative medicine as leading mean cell therapy. However, can not complete the task human body regeneration effectively by themselves far they need receptive microenvironment (the niche) engraft and perform properly. Understanding mechanisms underlying mammalian lead us an assumption that improved outcomes therapy requires specific generated...
Therapeutic angiogenesis is a promising strategy for relief of ischemic conditions, and gene delivery was used to stimulate blood vessels’ formation growth. We have previously shown that intramuscular injection mixture containing plasmids encoding vascular endothelial growth factor (VEGF)165 hepatocyte (HGF) leads restoration flow in mouse limb, efficacy combined superior each plasmid administered alone. In this work, we evaluated different approaches co-expression HGF VEGF165 genes panel...
Multipotent mesenchymal stem/stromal cells (MSC) are one of the crucial regulators regeneration and tissue repair possess an intrinsic program from self-organization mediated by condensation, migration self-patterning. The ability to self-organize has been successfully exploited in engineering approaches using cell sheets (CS) their modifications. In this study, we used CS as a model human MSC spontaneous demonstrate its structural, transcriptomic impact multipotent stromal commitment. We...
The article presents lecture materials on regeneration mechanisms from the textbook “Lectures Regenerative Medicine”. An example of epimorphic in mammals is considered — cellular tissue restoration fingertip after amputation. This model a relevant object research, since it allows us to study both formation specialized temporary structure blastema and subsequent morphogenesis, which ends with full all amputated tissues.
During each period, the uterine mucosa of women reproductive age heals without fibrosis. Previously, we established that soluble factors are released in this way have an antifibrotic effect on culture human endometrial mesenchymal stromal cells. The objective work was to evaluate properties these vitro fibrosis model. Serum menstrual and peripheral blood were obtained from a healthy donor one day. Mesenchymal cells endometrium also isolated blood. Simulation carried out by differentiation...
Regeneration is a fundamental perpetual tissue process much attributed to functions of adult stem cell. In last decades delivery cells restore structure widely adopted in regenerative medicine. However, can not complete the task human body regeneration effectively as far need basis form tissue. Certain improvement be achieved when are organized sheets (CS) – primitive tissue-engineered structures comprising viable and ECM proteins. This led us an assumption that after damage...
Cell sheet (CS) engineering using mesenchymal stromal cells (MSC) draws significant interest for regenerative medicine and this approach translates to clinical use numerous indications. However, little is known of factors that define the timing CS assembly from primary cultures. This aspect important planning delivery in autologous allogeneic modes use. We used a comparative vitro with donors' (n = 14) adipose-derived MSCs evaluated impact healthy subject's sex, MSC culture features...
Aim. The work was aimed to test whether the expression levels of endometrial-specific Hoxa10 and Hoxa11 genes in mouse uterus change after endometrial injury caused by giving birth, suggest a mechanism which these can be upregulated stromal cells injury. Methods. study performed using young (8–10 weeks old) wild-type mice C57BL6 line; gene uterine tissues assessed before delivery, as well 4 hours 24 delivery were also used work. Hypoxia modeled vitro human adding 200 mM CoCl2. Inhibition DNA...