A5045094534- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Protease and Inhibitor Mechanisms
- Fibroblast Growth Factor Research
- Endometriosis Research and Treatment
- Peptidase Inhibition and Analysis
- Genetics and Physical Performance
- Cardiac Structural Anomalies and Repair
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Occupational and environmental lung diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Reproductive System and Pregnancy
- Biomedical and Engineering Education
- Computational Drug Discovery Methods
- Adipose Tissue and Metabolism
- Kruppel-like factors research
- Signaling Pathways in Disease
- Wound Healing and Treatments
- Muscle Physiology and Disorders
- Tendon Structure and Treatment
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Cancer-related molecular mechanisms research
- Pulmonary Hypertension Research and Treatments
Lomonosov Moscow State University
2020-2025
Moscow State University
2023-2025
Regenerative Medicine Institute
2021
Institute of Molecular Medicine
2021
Extracellular matrix (ECM) provides both structural support and dynamic microenvironment for cells regulating their behavior fate. As a critical component of stem cell niche ECM maintains activates proliferation differentiation under specific stimuli. Mesenchymal stem/stromal (MSCs) regulate tissue-specific functions locating in immediate producing various bioactive factors, including components. We evaluated the ability MSC-produced to restore progenitor vitro analyzed possible mechanisms...
The development of tissue fibrosis is a complex process involving the interaction multiple cell types, which makes search for antifibrotic agents rather challenging. So far, myofibroblasts have been considered key type that mediated and thus was main target therapy. However, current strategies aimed at inhibiting myofibroblast function or eliminating them fail to demonstrate sufficient effectiveness in clinical practice. Therefore, today, there an unmet need more reliable cellular targets...
The study of molecular mechanisms regeneration requires convenient models for in vitro and vivo studies. In cell cultures can fulfill such a function. However, their rapid aging loss initial specific properties culture is significant limitation use. Telomerase expression help to overcome these limitations: it prolong proliferative activity stabilize the primary culture. Here, we created studied human adipose tissue multipotent mesenchymal stromal (MSC) that overexpress catalytic protein...
Besides certain exceptions, healing of most tissues in the human body occurs via formation scar tissue, rather than restoration lost structures. After extensive acute injuries, this phenomenon substantially limits possibility function recovery and, case chronic injury, it leads to pathological remodeling organs affected. Managing outcomes damaged tissue repair is one main objectives regenerative medicine. The first priority for reaching comparative investigation mechanisms responsible...
Multipotent mesenchymal stromal cells (MSCs) integrate hormone and neuromediator signaling to coordinate tissue homeostasis, renewal regeneration. To facilitate the investigation of MSC biology, stable immortalized cell lines are created (e.g., commercially available ASC52telo). However, ASC52telo line has an impaired adipogenic ability a depressed response hormones, including 5-HT, GABA, glutamate, noradrenaline, PTH insulin compared primary cells. This markedly reduces potential in...
Mesenchymal stromal cells (MSCs) are the key regulators of tissue homeostasis and repair after damage. Accumulating evidence indicates dual contribution MSCs into development fibrosis induced by chronic injury: these can suppress fibrotic process due to paracrine activity, but their promoting role in differentiating myofibroblasts has also been demonstrated. Many model systems reproducing have shown ability peroxisome proliferator-activated receptor (PPAR) agonists reverse myofibroblast...
Homeobox proteins (Hox) are universal regulators of body patterning process in embryogenesis metazoan. The Hox gene expression pattern (Hox code) retains adult tissues and serves as cellular positional identity marker. Despite previously existing notions that the code is inherent all stroma mesenchymal cells a whole, recent studies have shown may be an attribute distinct subpopulation resident stromal (MSC). Recent evidence allows to suggest "non-canonical" role for genes which associated...
T-cadherin is a regulator of blood vessel remodeling and angiogenesis, involved in adiponectin-mediated protective effects the cardiovascular system skeletal muscles. GWAS study has previously demonstrated SNP Cdh13 gene to be associated with hypertension. However, role regulating pressure not been experimentally elucidated. Herein, we generated Cdh13∆Exon3 mice lacking exon 3 described their phenotype. exhibited normal gross morphology, life expectancy, breeding capacity. Meanwhile, body...
Multipotent mesenchymal stromal cells (MSCs) regulate tissue repair through paracrine activity, with secreted proteins being significant contributors. Human commonly results in fibrosis, where fibroblast differentiation into myofibroblasts is a major cellular mechanism. MSCs’ activity can inhibit fibrosis development. We previously demonstrated that the separation of MSC secretome, represented by conditioned medium (CM), subfractions enriched extracellular vesicles (EV) or soluble factors...
The study aims to assess the role of EU biomedical research infrastructures in fight against COVID-19 pandemic and analyze their response challenges associated with spread new pathogen.Materials Methods.We analyzed materials Seventh Framework Program for Research Technological Development (FP7, 2007(FP7, -2013) ) Eighth "Horizon 2020" (FP8, 2014-2020), official reports European Strategic Forum on Infrastructures, expert reports, as well documents Commission, Data Portal, other relevant...
It is well known that mesenchymal stromal cells (MSCs) prevent the development of fibrosis in a paracrine manner; still, underlying mechanisms have not yet been studied. Using proteomic analysis, we identified DKK3 protein, regulator Wnt signaling pathway, MSC secretome. We also demonstrated protein secretome suppresses canonical pathway fibroblasts and prevents myofibroblast differentiation.
Cell sheet (CS) engineering using human MSC is of significant interest for regenerative medicine and this rapidly growing field translates to clinical use in a number indications. Nevertheless, little known factors that define the timing CS assembly from primary cultures which important planning treatments both – autologous allogeneic modes use. We used comparative vitro approach with donors’ (n=14) adipose-derived evaluated impact healthy subject’s characteristics (age sex), culture...
Establishing the molecular and cellular mechanisms of fibrosis requires development validated reproducible models. The complexity in vivo models challenges monitoring an individual cell fate, some cases making it impossible. However, set factors affecting cells vitro culture systems differ significantly from conditions, insufficiently reproducing living systems. Thus, to model profibrotic conditions , usually key factor, transforming growth factor beta (TGFβ-1) is used as a single factor....
Aim. The work was aimed to test whether the expression levels of endometrial-specific Hoxa10 and Hoxa11 genes in mouse uterus change after endometrial injury caused by giving birth, suggest a mechanism which these can be upregulated stromal cells injury. Methods. study performed using young (8–10 weeks old) wild-type mice C57BL6 line; gene uterine tissues assessed before delivery, as well 4 hours 24 delivery were also used work. Hypoxia modeled vitro human adding 200 mM CoCl2. Inhibition DNA...
Cell sheet (CS) engineering using mesenchymal stromal cells (MSC) draws significant interest for regenerative medicine and this approach translates to clinical use numerous indications. However, little is known of factors that define the timing CS assembly from primary cultures. This aspect important planning delivery in autologous allogeneic modes use. We used a comparative vitro with donors' (n = 14) adipose-derived MSCs evaluated impact healthy subject's sex, MSC culture features...