A5102856040- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Intracerebral and Subarachnoid Hemorrhage Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Adipokines, Inflammation, and Metabolic Diseases
- CAR-T cell therapy research
- Orbital Angular Momentum in Optics
- Nerve injury and regeneration
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- RNA and protein synthesis mechanisms
- Muscle Physiology and Disorders
- Signaling Pathways in Disease
- Adipose Tissue and Metabolism
- Near-Field Optical Microscopy
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- Cardiovascular Disease and Adiposity
Lomonosov Moscow State University
2020-2025
Moscow State University
2012-2025
The study of molecular mechanisms regeneration requires convenient models for in vitro and vivo studies. In cell cultures can fulfill such a function. However, their rapid aging loss initial specific properties culture is significant limitation use. Telomerase expression help to overcome these limitations: it prolong proliferative activity stabilize the primary culture. Here, we created studied human adipose tissue multipotent mesenchymal stromal (MSC) that overexpress catalytic protein...
Multipotent mesenchymal stromal cells (MSCs) integrate hormone and neuromediator signaling to coordinate tissue homeostasis, renewal regeneration. To facilitate the investigation of MSC biology, stable immortalized cell lines are created (e.g., commercially available ASC52telo). However, ASC52telo line has an impaired adipogenic ability a depressed response hormones, including 5-HT, GABA, glutamate, noradrenaline, PTH insulin compared primary cells. This markedly reduces potential in...
Multipotent mesenchymal stromal cells (MSCs) are considered to be critical contributors injured tissue repair and regeneration, MSC-based therapeutic approaches have been applied many peripheral central neurologic disorders. It has demonstrated that the beneficial effects of MSC mainly mediated by components their secretome. In current study, we explored neuroprotective potential secretome in a rat model intracerebral hemorrhage shown 10-fold concentrated human its combination with...
Multipotent stromal cells (MSC) demonstrate remarkable functional heterogeneity; however, its molecular mechanisms remain largely obscure. In this study, we explored MSC response to hormones, which activate Gs-protein / cyclic AMP (cAMP) protein kinase A (PKA) dependent signaling, at the single cell level using genetically encoded biosensor PKA-Spark. For first time, demonstrated that about half of cultured MSCs are not able cAMP/PKA pathway, possibly due limited availability adenylyl...
Intracerebral hemorrhage is an unmet medical need that often leads to the disability and death of a patient. The lack effective treatments for intracerebral makes it necessary look them. Previously, in our proof-of-concept study (Karagyaur M et al. Pharmaceutics, 2021), we have shown secretome multipotent mesenchymal stromal cells (MSC) provides neuroprotection brain model rats. Here, conducted systematic therapeutic potential MSC hemorrhagic stroke provided answers questions be addressed...
Hypertension is a major risk factor for cardiovascular diseases, such as strokes and myocardial infarctions. Nearly 70% of hypertension onsets in adults can be attributed to obesity, primarily due sympathetic overdrive the dysregulated renin-angiotensin system. Sympathetic increases vasoconstriction via α1-adrenoceptor activation on vascular cells. Despite fact that outflow individuals with rule, there cohort patients obesity who do not develop hypertension. In this study, we investigated...
Non-coding RNA (ncRNAs) genes have attracted increasing attention in recent years due to their widespread involvement physiological and pathological processes regulatory networks. The study of the function molecular partners ncRNAs opens up opportunities for early diagnosis treatment previously incurable diseases. However, classical “loss-of-function” approach ncRNA analysis is challenged some specific issues. Here, we studied potency two CRISPR/Cas9 variants, wild-type (SpCas9wt) nickase...
Brain-derived neurotrophic factor (BDNF) is a classic neuroprotective and pro-regenerative in peripheral central nervous tissue. Its ability to stimulate the restoration of damaged nerve brain tissue after ischemic stroke intraventricular hemorrhage has been demonstrated. However, current concept regeneration allows us assert that one factor, even if essential, cannot be sole contributor this complex biological process. We have previously shown urokinase-type plasminogen activator (uPA)...
Ischemic and hemorrhagic strokes, traumatic brain injury, bacterial viral encephalitis, toxic metabolic encephalopathies are very different pathologies. But, they have much more in common than it might seem at first glance. In this review, the authors propose to consider these pathologies from point of view unity their pathogenetic mechanisms approaches therapy. Particular attention is paid promising therapeutic approaches, such as therapy using cells secretion products: an analysis...
In this study, we developed a novel Cre/lox71-based system for the controlled transient expression of target genes. We used bacteriophage P1 Cre recombinase, which harbors short, highly specific DNA-binding site and does not have endogenous binding sites within mouse or human genomes. Fusing catalytically inactive form recombinase VP64 transactivation domain (VP16 tetramer), constructed artificial transcription factor Cre-VP64. This binds to lox71 promoter region gene and, therefore,...
Relevance . The use of gene therapy drugs for the treatment genetic diseases and stimulation regeneration processes is lengthy involves repeated injections, which may lead to increased dissemination constructs from injection site undesirable ectopic expression growth factors encoded in them. Existing approaches study pharmacokinetics a drug assess administration are not applicable. Objective : evaluate suitability real-time PCR method studying biodistribution promising mice during course...