A5077237348- Muscle Physiology and Disorders
- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- RNA Research and Splicing
- Marine Sponges and Natural Products
- Tardigrade Biology and Ecology
- RNA modifications and cancer
- Exercise and Physiological Responses
- Genetics and Physical Performance
- Invertebrate Immune Response Mechanisms
- Mitochondrial Function and Pathology
- Neurobiology and Insect Physiology Research
- Cephalopods and Marine Biology
- Single-cell and spatial transcriptomics
- Plant and Biological Electrophysiology Studies
- Biocrusts and Microbial Ecology
- Aquaculture disease management and microbiota
- Congenital limb and hand anomalies
- Protist diversity and phylogeny
- Bat Biology and Ecology Studies
- Planarian Biology and Electrostimulation
- Marine Biology and Environmental Chemistry
- Hedgehog Signaling Pathway Studies
- CRISPR and Genetic Engineering
- Marine Toxins and Detection Methods
Kazan Federal University
2016-2025
Innovation Cluster (Canada)
2018
Reduction in daily activity leads to dramatic metabolic disorders, while regular aerobic exercise training is effective for preventing this problem. The purpose of study was identify genes that are directly related contractile human skeletal muscle, regardless the level fitness. Transcriptome changes after one-legged knee extension exercised and contralateral nonexercised vastus lateralis muscle seven men were evaluated by RNA-seq. change at baseline 2 mo (5/wk, 1 h/day) as well....
Opportunistic bacteria Staphylococcus aureus and epidermidis often form rigid biofilms on tissues inorganic surfaces. In the biofilm bacterial cells are embedded in a self-produced polysaccharide matrix thereby inaccessible to biocides, antibiotics, or host immune system. Here we show antibacterial activity of newly synthesized cationic quaternary ammonium, bisphosphonium salts pyridoxine (vitamin B6) against biofilm-embedded Staphylococci. The derivatives 6-hydroxymethylpyridoxine were...
Abstract Background More than half of human protein-coding genes have an alternative transcription start site (TSS). We aimed to investigate the contribution TSSs acute-stress-induced transcriptome response in tissue (skeletal muscle) using cap analysis gene expression approach. were examined at baseline and during recovery after acute stress (a cycling exercise). Results identified 44,680 CAGE TSS clusters (including 3764 first defined) belonging 12,268 annotated for time 290 163 genes. The...
The study of molecular mechanisms regeneration requires convenient models for in vitro and vivo studies. In cell cultures can fulfill such a function. However, their rapid aging loss initial specific properties culture is significant limitation use. Telomerase expression help to overcome these limitations: it prolong proliferative activity stabilize the primary culture. Here, we created studied human adipose tissue multipotent mesenchymal stromal (MSC) that overexpress catalytic protein...
Abstract Regular low intensity aerobic exercise (aerobic training) provides effective protection against various metabolic disorders. Here, the roles played by transient transcriptome responses to acute and changes in baseline gene expression during up-regulation of protein content human skeletal muscle were investigated after 2 months training. Seven untrained males involved a month cycling training program. Mass-spectrometry RNA sequencing used evaluate proteome exercise. We found that...
Three-day dry immersion (a model of physical unloading) substantially changes the transcriptomic profile in human soleus muscle, a muscle with predominantly slow-twitch fibers and strong postural function; despite this, we found no effect on proteome (highly abundant proteins). Dry markedly reduces ADP-stimulated respiration; this decline is not accompanied by decrease content mitochondrial proteins/respiratory enzymes, indicating disruption regulation cellular respiration.
Abstract Non-biting midges (Chironomidae) are known to inhabit a wide range of environments, and certain species can tolerate extreme conditions, where the rest insects cannot survive. In particular, sleeping chironomid Polypedilum vanderplanki is for remarkable ability its larvae withstand almost complete desiccation by entering state called anhydrobiosis. Chromosome numbers in chironomids higher than other dipterans this extra genomic resource might facilitate rapid adaptation novel...
We showed previously that inhibition of KIT signaling in GISTs activates FGFR-signaling pathway rendering cancer cells resistant to receptor tyrosine kinase inhibitor (RTKi) imatinib mesylate (IM) (Gleevec) despite absence secondary mutations and thereby illustrating a rationale for the combined (e.g., KIT- FGFR-targeted) therapies. show here long-term culture IM-resistant (GIST-R1) with IM substantially down-regulates expression induces activation cascade, evidenced by increased total...
Background: Immune mechanisms recently emerged as important contributors to migraine pathology with cytokines affecting neuronal excitation. Therefore, elucidating the profile of activated in various forms migraine, including those a known genetic cause, can help diagnostic and therapeutic approaches. Methods: Here we (i) performed exome sequencing identify causal gene mutation (ii) measured, using Bio-Plex technology, 22 serum patients familial (two hemiplegic two aura) from Russian family...
Synthetic promoters are vital for genetic engineering-based strategies crop improvement, but effective methodologies their creation and systematic testing lacking. We report here on the comparative analysis of pro-SmAMP1 pro-SmAMP2 from Stellaria media ANTIMICROBIAL PEPTIDE1 (AMP1) PEPTIDE2 (AMP2). These more than well-known Cauliflower mosaic virus 35S promoter. Although these share about 94% identity, promoter demonstrated stronger transient expression a reporter gene in Agrobacterium...
The ability to regulate oxygen consumption evolved in ancestral animals and is intrinsically linked iron metabolism. pathways have been intensively studied mammals, whereas data on distant invertebrates are limited. Sea sponges represent the oldest animal phylum unique structural plasticity capacity reaggregate after complete dissociation. We metabolic factors their expression during reaggregation White cold-water Halichondria panicea Halisarca dujardini. De novo transcriptomes were...
Ferritins comprise a conservative family of proteins found in all species and play an essential role resistance to redox stress, immune response, cell differentiation. Sponges (Porifera) are the oldest Metazoa that show unique plasticity regenerative potential. Here, we characterize ferritins two cold-water sponges using proteomics, spectral microscopy, bioinformatic analysis. The recently duplicated HdF1a/b atypical HdF2 genes were Halisarca dujardini genome. Multiple related transcripts...
Abstract Organization and functioning of immune system remain unevenly studied in different taxa lophotrochozoan animals. We analyzed transcriptomic data on coelomocytes the lugworm Arenicola marina (Linnaeus, 1758; Annelida, Polychaeta) to gain insights into molecular mechanisms involved polychaete immunity. Coelomocytes are specialized motile cells populating coelomic fluid annelids, responsible for cellular defense reactions providing humoral factors. The transcriptome was enriched with...
The development of craniofacial skeletal structures is fascinatingly complex and elucidation the underlying mechanisms will not only provide novel scientific insights, but also help develop more effective clinical approaches to treatment and/or prevention numerous congenital malformations. To this end, we performed a genome-wide analysis RNA transcription from non-coding regulatory elements by CAGE-sequencing facial mesenchyme human embryos cross-checked active enhancers thus identified...
Introduction The remarkable diversity observed in the structure and development of molluscan nervous system raises intriguing questions regarding molecular mechanisms underlying neurogenesis Mollusca . expression SoxB family transcription factors plays a pivotal role neuronal development, thereby offering valuable insights into strategies neurogenesis. Methods In this study, we conducted gene analysis focusing on SoxB-family during early gastropod Lymnaea stagnalis We employed combination...
Genomic safe harbors (GSHs) provide ideal integration sites for generating transgenic organisms and cells can be of great benefit in advancing the basic applied biology a particular species. Here we report identification GSHs dry-preservable insect cell line, Pv11, which derives from sleeping chironomid, Polypedilum vanderplanki, similar to larvae its progenitor species exhibits extreme desiccation tolerance. To identify GSHs, carried out genome analysis lines established by random exogenous...
Abstract The sleeping chironomid Polypedilum vanderplanki is capable of anhydrobiosis, a striking example adaptation to extreme desiccation. Tolerance complete desiccation in this species associated with emergence multiple paralogs protective genes. One the gene families highly expressed under anhydrobiosis and involved process protein-L-isoaspartate (D-aspartate) O-methyltransferases (PIMTs). Recently, another closely related midge was discovered, pembai, which able not only tolerate but...
Abstract Upregulation of glycolysis and downregulation mitochondrial oxidative phosphorylation, termed as the Warburg effect, are characteristic tumor cells 1,2 . Restriction pyruvate flux into matrix is one major mechanisms underlying this phenomenon 3 Warburg-type metabolism beneficial for rapidly proliferating cells, however its function remains unclear. Moreover, it unknown what metabolic consequences activation respiration in cancer are. Here we created a chemogenetic instrument,...
Abstract Background Triphalangeal thumb-polysyndactyly syndrome (TPT-PS) is a rare well-defined autosomal dominant disorder characterized by long thumbs with three phalanges combined pre- and postaxial polydactyly/syndactyly of limbs. By now, the has been reported in several large families from different ethnic backgrounds, high degree inter- intrafamilial variability. The genome locus responsible for TPT-PS mapped to 7q36.3 region harboring long-range sonic hedgehog (SHH) regulatory...
The prevention of muscle atrophy carries with it clinical significance for the control increased morbidity and mortality following physical inactivity. While major transcriptional events associated atrophy-recovery processes are subject active research on gene level, contribution non-coding regulatory elements alternative promoter usage is a source both production protein products new insights into activity transcription factors. We used cap-analysis expression (CAGE) to create genome-wide...
Abstract Mice enter an active hypometabolic state, called daily torpor when they experience a lowered caloric intake under cold ambient temperature. During torpor, the oxygen consumption rate in some animals drops to less than 30% of normal without harming body. This safe but severe reduction metabolism is attractive for various clinical applications; however, mechanism and molecules involved are unclear. Therefore, here we systematically analyzed gene expression landscape on level RNA...
Tay-Sachs disease (TSD) is a rare genetic disorder with diverse clinical manifestations, often leading to underdiagnosis due symptom similarities other neurological conditions. In this study, we aimed identify the mutations underlying late-onset TSD in 27-year-old patient progressive symptoms. Whole-exome sequencing revealed two hexA gene associated TSD: previously known variant, c.805G > A (p.Gly269Ser), and novel splice-site mutation, c.346 + 2dupT. Through assessments, analysis,...