A5004986985- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- Angiogenesis and VEGF in Cancer
- Protease and Inhibitor Mechanisms
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Congenital heart defects research
- Cardiac Fibrosis and Remodeling
- Blood Coagulation and Thrombosis Mechanisms
- Cell Adhesion Molecules Research
- Cardiovascular Disease and Adiposity
- Cerebrovascular and Carotid Artery Diseases
- Coronary Interventions and Diagnostics
- Atherosclerosis and Cardiovascular Diseases
- Metabolism, Diabetes, and Cancer
- Lipoproteins and Cardiovascular Health
- Extracellular vesicles in disease
- Kruppel-like factors research
- Virus-based gene therapy research
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
Lomonosov Moscow State University
2016-2025
National Medical Research Center of Cardiology
2017-2025
V.A. Negovsky Scientific Research Institute of General Reanimatology
2025
Institute of Experimental Cardiology
2013-2024
Moscow State University
2018-2021
National Tsing Hua University
2019
Ministry of Health of the Russian Federation
1999-2008
University of Chicago
2006
University of Rochester Medical Center
2006
Institute of Cytology
2004
Transplantation of adipose-derived mesenchymal stem cells (ASCs) induces tissue regeneration by accelerating the growth blood vessels and nerve. However, mechanisms which they accelerate nerve fibers are only partially understood. We used transplantation ASCs with subcutaneous matrigel implants (well-known in vivo model angiogenesis) mice limb reinnervation to check influence ASC on growth. Here we show that stimulate nerves innervated mice's limbs induce axon implants. To investigate...
Adipose-derived stromal cells (ASCs) are suggested to be potent candidates for cell therapy of ischemic conditions due their ability stimulate blood vessel growth. ASCs produce many angiogenic and anti-apoptotic growth factors, secretion is significantly enhanced by hypoxia. Utilizing a Matrigel implant model, we showed that hypoxia-treated stimulated angiogenesis as well maturation the newly formed vessels in vivo. To elucidate mechanisms ASC action, used co-culture model with isolated from...
Tissue regeneration is impaired in aged individuals. Adipose-derived mesenchymal stromal cells (ADSCs), a promising source for cell therapy, were shown to secrete various angiogenic factors and improve vascularization of ischemic tissues. We analyzed how patient age affected the properties ADSCs. ADSCs isolated from subcutaneous fat tissue patients with coronary artery disease (CAD; n = 64, 43-77 years old) without CAD (n 31, 2-82 old). ADSC phenotype characterized by flow cytometry was...
BACKGROUND: Chronic heart failure (CHF) is increasing in prevalence. Patients with CHF usually have co-morbid conditions, but these been subjected to little research and consequently there a paucity of guidance on how manage them. Obesity diabetes mellitus are common antecedents often complicate management influence outcome. Cachexia an ominous missed sign patients CHF. METHODS: This manuscript describes the rationale design Studies Investigating Co-morbidities Aggravating Heart Failure...
Modified cell-based angiogenic therapy has become a promising novel strategy for ischemic heart and limb diseases. Most studies focused on myoblast, endothelial cell progenitors or bone marrow mesenchymal stromal cells transplantation. Yet adipose-derived (in contrast to marrow) are abundantly available can be easily harvested during surgery liposuction. Due high paracrine activity availability ADSCs appear preferable type cardiovascular therapy. Still neither genetic modification of human...
Multipotent mesenchymal stem/stromal cells (MSC) including adipose-derived stromal (ADSC) have been successfully applied for cardiovascular diseases treatment. Their regenerative potential is considered due to the multipotency, paracrine activity and immunologic privilege. However, therapeutic efficacy of autologous MSC myocardial ischemia therapy modest. We analyzed if ADSC properties are attenuated in patients with chronic such as coronary artery disease (CAD) diabetes mellitus type 2...
Tissue regeneration requires coordinated "teamwork" of growth factors, proteases, progenitor and immune cells producing inflammatory cytokines. Mesenchymal stem (MSC) might play a pivotal role by substituting or secretion factors cytokines, attraction cells, which participate in initial stages tissue repair. Due to obvious impact inflammation on it seems promising explore whether could influence proangiogenic abilities MSC. In this study we investigated effects TNF-α activity adipose-derived...
Background: Peripheral nerve regeneration requires coordinated functions of neurotrophic factors and neuronal cells.CRISPR activation (CRISPRa) is a powerful tool that exploits inactive Cas9 (dCas9), single guide RNA (sgRNA) transcription activator for gene activation, but has yet to be harnessed tissue regeneration.Methods: We developed hybrid baculovirus (BV) vector harbor deliver the CRISPRa system multiplexed 3 factor genes (BDNF, GDNF NGF).The BV was used transduce rat adipose-derived...
We showed previously that increased urokinase plasminogen activator (uPA) expression contributes to vascular smooth muscle cell (VSMC) proliferation and neointima formation after injury. Proliferation of cultured rat aortic VSMCs induced by uPA was inhibited the antioxidant ebselen. Because increases in VSMC reactive oxygen species (ROS) contribute proliferation, we hypothesized ROS generation regulating or activity cellular oxidases.uPA stimulated production levels equivalent angiotensin II...
Increased interest in development of combined gene therapy emerges from results recent clinical trials that indicate good safety yet unexpected low efficacy "single-gene" administration. Multiple studies showed vascular endothelial growth factor 165 aminoacid form (VEGF165) and hepatocyte (HGF) can be used for induction angiogenesis ischemic myocardium skeletal muscle. Gene transfer system composed a novel cytomegalovirus-based (CMV) plasmid vector codon-optimized human VEGF165 HGF genes...
The adult heart contains small populations of multipotent cardiac progenitor cells (CPC) that present a convenient and efficient resource for treatment myocardial infarction. Several clinical studies direct CPC delivery by injection have already been performed but showed low engraftment rate limited beneficial effects procedure. «Cell sheet» technology has developed to facilitate longer retention grafted show new directions cell-based therapy using this strategy. In study we hypothesized...
Cell therapy using adipose-derived stromal cells (ADSC) is an intensively developing approach to promote angiogenesis and regeneration. Administration technique crucial among others minimal constructs - cell sheets (CS) have certain advantages. Delivery of CS allows transplantation along with matrix proteins facilitate engraftment. Cells' therapeutic potential can be also increased by expression proangiogenic factors viral transduction. In this work we report on efficacy from mouse ADSC...
Since development of plasmid gene therapy for therapeutic angiogenesis by J. Isner this approach was an attractive option ischemic diseases affecting large cohorts patients. However, first placebo-controlled clinical trials showed its limited efficacy questioning further advance to practice. Thus, combined methods using delivery several angiogenic factors got into spotlight as a way improve outcomes. This study provides experimental proof concept simultaneous VEGF165 and HGF genes alleviate...
Despite advancements in investigation of cardiac fibrosis mechanisms, molecular and cellular regulation this process remains insufficiently explored. In context, examination novel targets capable regulating the state fibroblasts their profibrotic properties becomes a pivotal area research. These processes include autophagy as an evolutionary conservative mechanism contributing to maintenance homeostasis through utilization macromolecules organelles via lysosomal degradation pathway. The...