A5021106919- Spine and Intervertebral Disc Pathology
- Mesenchymal stem cell research
- Spinal Cord Injury Research
- Osteoarthritis Treatment and Mechanisms
- Musculoskeletal pain and rehabilitation
- Periodontal Regeneration and Treatments
- Telomeres, Telomerase, and Senescence
- Medical Imaging and Analysis
- Tissue Engineering and Regenerative Medicine
- Gut microbiota and health
- Stroke Rehabilitation and Recovery
- Veterinary Orthopedics and Neurology
- Cervical and Thoracic Myelopathy
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Child Nutrition and Feeding Issues
- Diet and metabolism studies
- Traumatic Brain Injury Research
- Wound Healing and Treatments
- Pregnancy-related medical research
- Virus-based gene therapy research
- Cerebral Palsy and Movement Disorders
- Diabetic Foot Ulcer Assessment and Management
- Pressure Ulcer Prevention and Management
- Williams Syndrome Research
Policlinico Tor Vergata
2025
Swiss Paraplegic Research
2015-2024
University Hospital of Bern
2023-2024
University of Bern
2013-2024
RELX Group (United Kingdom)
2014
Biomechanics Institute of Valencia
2014
RELX Group (United States)
2014
Swiss Paraplegic Center
2011
Abstract Background Accurate identification of bacterial communities is crucial for research applications, diagnostics, and clinical interventions. Although 16S ribosomal RNA (rRNA) gene sequencing a widely employed technique taxonomic classification, it often results in misclassified or unclassified taxa. This study sought to refine the full-length rRNA protocol using MinION sequencer, focusing on V1–V9 regions. Our methodological enquiry examined several factors, including number PCR...
There is evidence that mesenchymal stem cells (MSCs) can differentiate towards an intervertebral disc (IVD)-like phenotype. We compared the standard chondrogenic protocol using transforming growth factor beta-1 (TGFß) to effects of hypoxia, and differentiation factor-5 (GDF5), coculture with bovine nucleus pulposus (bNPC). The efficacy molecules recently discovered as possible (NP) markers between IVD-like was evaluated. MSCs were isolated from human bone marrow encapsulated in alginate...
Mesenchymal stromal cells (MSC) are used in cell therapies, however cellular senescence increases heterogeneity of populations and leads to uncertainty therapies' outcomes. The determination is time consuming logistically intensive. Here, we propose the use endogenous autofluorescence as real-time quantification human MSC, based on label-free flow cytometry analysis. We correlated using senescence-associated beta-galactosidase assay (SA-β-Gal) with chromogenic (X-GAL) fluorescent (C12FDG)...
Human bone marrow-derived mesenchymal stem cells (MSCs) have limited growth potential in vitro and cease to divide due replicative senescence, which from a tissue-engineering perspective has practical implications, such as defining the correct starting points for differentiation transplantation. Time spent culture before loss of required is different reflects patient variability, problem cell expansion. This study aimed develop score set can be used quantify senescent state MSCs predict...
Despite advancements in breast cancer surgery, the decision-making process for axillary treatment remains complex, necessitating new predictors like tumor size to Ki67 proliferation index ratio. Intraoperative examination of sentinel lymph node is performed reduce risk a secondary surgery. Several studies have demonstrated that even presence moderate nodal involvement, local disease control can be achieved by omitting dissection (ALND). The aim our retrospective study compare patients...
Degeneration of intervertebral discs (IVDs) is associated with back pain and elevated levels inflammatory cells. It has been hypothesised that discogenic a direct result vascular neural ingrowth along annulus fissures, which may expose the avascular nucleus pulposus (NP) to systemic circulation induce an autoimmune reaction. In this study, we confirmed our previous observation antibodies in human degenerated post-traumatic IVDs cultured vitro. We presence was due reaction against specific...
The study of canine bone marrow-derived mesenchymal stem cells (MSCs) has a prominent position in veterinary cell-based applications. Yet the plethora breeds, their different life spans, and interbreed variations provide unclearness on what can be achieved specifically by such therapies. In this study, we compared set morphological, physiological, genetic markers MSCs derived from large dog namely, Border collie, German shepherd, Labrador, Malinois, Golden retriever, Hovawart. We...
Abstract Disorders related to gut health are a significant cause of morbidity among athletes in wheelchair. This pilot feasibility trial aims investigate whether probiotics compared prebiotics can improve inflammatory status and microbiome composition elite We conducted 12-week, randomized, cross-over controlled involving 14 Swiss Participants were given multispecies-multistrain probiotic or prebiotic (oat bran) daily for 4 weeks (Clinical trials.gov NCT04659408 09/12/2020). was followed by...
The intradiscal application of mesenchymal stem cells (MSCs) is considered a promising strategy for intervertebral disc (IVD) regeneration. Although many studies have been published, the feasibility and regenerative effects MSC not evaluated in an animal model suffering from naturally occurring degenerative disease. Six German Shepherd Dogs IVD disease were included. Autologous MSCs isolated bone marrow (iliac crest) cultured 3 weeks. After decompressive spinal surgery, three dogs received...
Abstract Mesenchymal stem cells (MSCs) are expected to have a fundamental role in future cell-based therapies because of their high proliferative ability, multilineage potential, and immunomodulatory properties. Autologous transplantations the “elephant room” problem wide donor variability, reflected by variability MSC quality characteristics, leading uncertain outcomes use these cells. We propose life imaging as tool characterize populations human MSCs. Bone marrow MSCs from various donors...
Human bone marrow-derived mesenchymal stem cells (MSC) are adult progenitor with great potential for application in cell-based therapies. From a therapy perspective, there two limitations to MSC use: (1) these therapies require large numbers of cells, and long-term expansion vitro promotes replicative senescence; (2) patient variability is challenge defining quality standards transplantation. This study aimed determine whether low or high oxidative status correlate changes cell...
Individuals with a spinal cord injury (SCI), despite specialized rehabilitation and good health care, have reduced life expectancy. Infectious diseases, such as pneumonias, infected pressure sores urinary tract infections (UTI) been identified the leading causes of mortality. We hypothesise that premature onset immune frailty occurs in SCI, possibly caused also by recurrent infections. A cross sectional study was performed comparing blood urine samples between able bodied controls (n = 84)...
Tissue engineering is a field in progressive expansion and requires constant updates methods devices. One of the central fields development biocompatible, biodegradable, injectable scaffolds, such as collagen microcarriers. To enhance cell attachment produce cost-effective culture solution with local stimulation cells, basic fibroblast growth factor (bFGF) or transforming factor-β1 (TGF-β1) was covalently immobilized on microcarriers either by 1-ethyl-3-(3-dimethylaminopropyl)...
Chronic back pain is one of the most important socioeconomic problems that affects global population. Elevated levels inflammatory mediators, such as cytokines, have been correlated with pain, but their role in chronic remains unclear. The effectiveness anti-inflammatory drugs seems to be limited for pain. authors wanted investigate mediators long-term medically treated patients persistent pain.Cytokine plasma (n=23), compared pain-free healthy controls (n=30), were investigated by...
Degeneration of the intervertebral disc (IVD) is a progressive and chronic process, high incidence discogenic disorders calls for new therapeutic approaches, such as cell-based therapies using three dimensional cultures mesenchymal stem cells (MSC), which can differentiate to chondrogenic-and IVD-lineages.Here, we investigated growth differentiation human MSC culture on biodegradable collagen scaffolds in order obtain an injectable suspension.Commercially available wound dressings were...
Abstract Mesenchymal stromal cells (MSC) are used in cell therapy, but results depend on the unknown quality of populations. Extended culture time MSC increases their senescent levels, leading to a critical loss fitness. Here, we tested suitability MSC-sorting based FACS autofluorescence profile, for rapid and non-invasive method elimination. Cells were classified low- (LA) high- (HA) groups, compared original population (control). Three days after sorting, screened by replicative senescence...
Degeneration of intervertebral discs (IVD) is one the main causes back pain and tissue engineering has been proposed as a treatment.Tissue requires use highly expensive growth factors, which might, in addition, lack regulatory approval for human use.In an effort to find readily available differentiation we tested three molecules -dexamethasone, triiodothyronine (T3) insulin -on IVD cells isolated after surgery, expanded vitro transferred into alginate beads.Triplicates containing 40 ng/ml...