A5067822190- Photosynthetic Processes and Mechanisms
- ATP Synthase and ATPases Research
- Lipid Membrane Structure and Behavior
- Photoreceptor and optogenetics research
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Light effects on plants
- Photochemistry and Electron Transfer Studies
- Enzyme Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Inflammasome and immune disorders
- Fungal Infections and Studies
- Plant-Microbe Interactions and Immunity
- Metalloenzymes and iron-sulfur proteins
- Legionella and Acanthamoeba research
- Water Treatment and Disinfection
- Antifungal resistance and susceptibility
- Fatigue and fracture mechanics
- Ion Transport and Channel Regulation
- Biochemical and Molecular Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Connective Tissue Growth Factor Research
- Heme Oxygenase-1 and Carbon Monoxide
- Microbial Metabolic Engineering and Bioproduction
Van Andel Institute
2020-2025
Chongqing Technology and Business University
2025
University of Kentucky
2017-2022
Hebei Medical University
2018-2019
The biological reduction of dinitrogen (N2) to ammonia (NH3) by nitrogenase is an energetically demanding reaction that requires low-potential electrons and ATP; however, pathways used deliver the from central metabolism reductants nitrogenase, ferredoxin or flavodoxin, remain unknown for many diazotrophic microbes. FixABCX protein complex has been proposed reduce flavodoxin using NADH as electron donor in a process known bifurcation. Herein, Azotobacter vinelandii was purified demonstrated...
Tubular injury is one of the crucial determinants progressive renal failure in diabetic nephropathy (DN), while epithelial-to-mesenchymal transition (EMT) tubular cells contributes to accumulation matrix protein kidney. Activation nucleotide binding and oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome leads maturation interleukin (IL)-1B involved pathogenic mechanisms diabetes. In this study, we explored role NLRP3 on high glucose (HG) or...
The P4 ATPases use ATP hydrolysis to transport large lipid substrates across bilayers. structures of the endosome- and Golgi-localized phosphatidylserine flippases—such as yeast Drs2 human ATP8A1—have recently been reported. However, a substrate-binding site on cytosolic side has not found, mechanisms with other are unknown. Here, we report S. cerevisiae Dnf1–Lem3 Dnf2–Lem3 complexes. We captured substrate phosphatidylcholine molecules both exoplasmic sides found that they have similar...
Accumulating evidence shows that circular RNAs (circRNAs) plays vital roles in tumor progression. However, the biological functions of circRNAs laryngeal squamous cell carcinoma (LSCC) metastasis is still unclear.qRT-PCR was used to detect circFLNA, miRNAs and FLNA mRNA expression. Transwell assay western blot were performed evaluate migration ability FLNA, MMP2 MLK1 protein expression, respectively. RNA pull-down analysis find binding-miRNAs circFLNA. Luciferase reporter examine effect...
Fungal infections cause millions of deaths annually and are challenging to treat due limited antifungal options increasing drug resistance. Cryptococci intrinsically resistant the latest generation antifungals, echinocandins, while Candida auris , a notorious global threat, is also increasingly resistant. We performed natural product extract screen for rescue activity echinocandin caspofungin against Cryptococcus neoformans H99, identifying butyrolactol A, which restores efficacy fungal...
Neomycin, an aminoglycoside antibiotic, has robust antibacterial properties, yet its clinical utility is curtailed by nephrotoxicity and ototoxicity. The mechanism which the polycationic neomycin enters specific eukaryotic cell types remains poorly understood. In budding yeast, NEO1 required for resistance encodes a phospholipid flippase that establishes membrane asymmetry. Here, we show mutations altering Neo1 substrate recognition cause hypersensitivity exposing...
Abstract P4 ATPases are lipid flippases that phylogenetically grouped into P4A, P4B and P4C clades. The P4A heterodimers composed of a catalytic α-subunit accessory β-subunit, the structures several heterodimeric have been reported. S. cerevisiae Neo1 its orthologs represent ATPases, which function as monomeric without β-subunit. It has unclear whether retain architecture transport mechanism dimeric flippases. Here we report structure ATPase, Neo1, in E1-ATP, E2P-transition, E2P states....
Flavin-based electron bifurcation allows enzymes to redistribute energy among electrons by coupling endergonic and exergonic transfer reactions. Diverse bifurcating employ a two-flavin flavoprotein (ETF) that accepts hydride from NADH at flavin (the so-called FAD, Bf-FAD). The Bf-FAD passes one exergonically second thereby assuming reactive semiquinone state able reduce ferredoxin or flavodoxin semiquinone. it on via is known as the FAD (ET-FAD) believed correspond single present in...
Abstract Arl1 is an Arf-like (Arl) GTP-binding protein that interacts with the guanine nucleotide exchange factor Gea2 to recruit golgin Imh1 Golgi. The Arl1–Gea2 complex also binds and activates phosphatidylserine flippase Drs2 these functions may be related, although underlying molecular mechanism unclear. Here we report high-resolution cryo-EM structures of full-length complex. a large 1459 residues composed six domains (DCB, HUS, SEC7, HDS1-3). We show assembles stable dimer via...
A remarkable charge transfer (CT) band is described in the bifurcating electron flavoprotein (Bf-ETF) from Rhodopseudomonas palustris (RpaETF). RpaETF contains two FADs that play contrasting roles bifurcation. The Bf-FAD accepts electrons pairwise NADH, directs one to a lower-reduction midpoint potential (E°) carrier, and other higher-E° FAD (ET-FAD). Previous work noted CT at 726 nm formed when ET-FAD was reduced oxidized, suggesting both flavins participate. However, existing crystal...
ABSTRACT P4 ATPases are lipid flippases that phylogenetically grouped into P4A, P4B and P4C clades. The P4A heterodimers composed of a catalytic α-subunit accessory β-subunit, the structures several heterodimeric have been reported. S. cerevisiae Neo1 its orthologs represent ATPases, which function as monomeric without β-subunit. It has unclear whether retain architecture transport mechanism dimeric flippases. Here we report first structure ATPase, Neo1, in E1-ATP, E2P-transition, E2P...