A5086861459- Supramolecular Self-Assembly in Materials
- Protein Structure and Dynamics
- Bacteriophages and microbial interactions
- Enzyme Structure and Function
- Virus-based gene therapy research
- Porphyrin and Phthalocyanine Chemistry
- Polydiacetylene-based materials and applications
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Luminescence and Fluorescent Materials
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Alzheimer's disease research and treatments
- Glycosylation and Glycoproteins Research
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Crystallography and molecular interactions
- Nanofabrication and Lithography Techniques
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Electrochemical sensors and biosensors
- Antimicrobial Peptides and Activities
- Photochromic and Fluorescence Chemistry
University of Crete
2015-2024
FORTH Institute of Electronic Structure and Laser
2014-2024
Foundation for Research and Technology Hellas
2014-2023
National and Kapodistrian University of Athens
2023
University Hospital of Heraklion
2017-2019
Institute of Electronics
2017
University of Cyprus
2009
Laser Zentrum Hannover
2007
Université Grenoble Alpes
1999-2007
Centre National de la Recherche Scientifique
1987-2007
Amino acid substitutions at a site in the center of bacteriophage protein P22 tailspike polypeptide chain suppress temperature-sensitive folding mutations many sites throughout chain. Characterization intracellular and assembly process reveals that suppressors act pathway, inhibiting aggregation an early intermediate into kinetically trapped inclusion body state. The alone increase efficiency otherwise wild-type without altering stability or activity native These amino identify unexpected...
Molecular self-assembly is emerging as a viable "bottom-up" approach for fabricating nanostructures. Self-assembled biomolecular structures are particularly attractive, due to their versatile chemistry, molecular recognition properties, and biocompatibility. Among them, amyloid protein peptide fibrils self-assembled nanostructures with unique physical chemical stability, formed from quite simple building blocks; ability work template the fabrication of low resistance, conducting nanowires...
Diphenylalanine (FF) is a very common peptide with many potential applications, both biological and technological, due to large number of different nanostructures which it attains. The current work concerns detailed study the self-assembled structures FF in two solvents, an aqueous (H2O) organic (CH3OH) through simulations experiments. Detailed atomistic molecular dynamics (MD) solvents have been performed, using explicit solvent model. self-assembling propensity water obvious while methanol...
Abstract The ability to develop a rational basis for the binding of inorganic materials specific sites within self‐assembling biological scaffolds has important applications in nanobiotechnology. Amyloid‐forming peptides are class such and show enormous potential as templates fabrication low resistance, conducting nanowires. Here we report use peptide building block scaffold systematic introduction metal‐binding residues at locations structure. octapeptide NSGAITIG...
Abstract Artificial light-harvesting systems have until now not been able to self-assemble into structures with a large photon capture cross-section that upon stimulus reversibly can switch an inactive state. Here we describe simple and robust F L -dipeptide construct which meso -tetraphenylporphyrin has appended self-assembles fibrils, platelets or nanospheres depending on the solvent composition. The functioning as quenched antennas, give intense excitonic couplets in electronic circular...
This article describes the combination of self-assembled peptide nanofibrils with metal electrodes for development an electrochemical metal-ion biosensor. The biological were immobilized on gold and used as biorecognition elements complexation copper ions. These obtained under aqueous conditions, at room temperature outside clean room. functionalized electrode was evaluated by cyclic voltammetry, impedance spectroscopy, energy dispersive X-ray atomic force microscopy. results displayed a...
Antimicrobial resistance (AMR) poses a significant global health risk as consequence of misuse antibiotics. Owing to the increasing antimicrobial resistance, it became imperative develop novel molecules and materials with properties. Porphyrins metalloporphyrins are compounds which present properties especially after irradiation. As consequence, porphyrinoids have recently been utilized agents in photodynamic inactivation bacteria other microorganisms. Herein, we report encapsulation...
The reversibility of the unfolding-refolding transition horse muscle phosphoglycerate kinase, induced by guanidine hydrochloride (Gdn X HCl), was studied using regain enzyme activity as a probe native structure. An irreversibility in reactivation process detected when protein incubated critical concentration denaturant (0.7 +/- 0.1 M Gdn HCl). This apparent observed for unfolding (N----D) well refolding (D----N). formation trough followed biphasic kinetics at 23 degrees C, first phase...
A series of protected phenylalanine and diphenylalanine derivatives have been coupled through a peptide bond to monoaminoporphyrin form new materials. comparative study in solution the solid state has performed confirmed interesting properties for self-assembled hybrid materials while conserving electronic chromophore. Thus, they are powerful candidates use dye-sensitized solar cells.
In this study, we propose a new approach to hard tissue regeneration based on the mineralization of 3D scaffolds made using lasers. To end, report rational design aspartate-containing self-assembling peptides targeted for calcium binding. We further investigate suitability these support cell attachment and proliferation when coupled hybrid organic-inorganic structurable material, evaluate response pre-osteoblastic cells functionalized material surfaces. Our results show that mineralized...
Self-assembled peptides gain increasing interest as biocompatible and biodegradable scaffolds for tissue engineering. Rationally designed self-assembling building blocks that carry cell adhesion motifs such Arg-Gly–Asp (RGD) are especially attractive. We have used a combination of theoretical experimental approaches toward rational designs, focusing on modular designs consist central ultrashort amphiphilic motif derived from the adenovirus fiber shaft. In this study, we rationally...
In this work a peptide nucleic acid has been covalently linked with porphyrin and boron-dipyrromethene exhibiting self assembly properties light-harvesting capability.
Two hybrid materials were designed by conjugating peptide nucleic acids (PNAs) to porphyrin or boron-dipyrromethene, generating PNA-porphyrin (PNA-TPP) and PNA-BODIPY (PNA-BDP) conjugates, respectively. Because of the combination supramolecular characteristics PNAs photosensitizers, two conjugates readily self-assemble in aqueous solutions produce well-defined nanoparticles with uniform particle sizes. The resulting kinds show good stability biological upon dilution. Importantly, can...
In this work, the diphenylalanine dipeptide was covalently attached to a tripyridyl porphyrin macrocycle and conveyed self-assembling properties resulting hybrid. This hybrid metallated with non-noble metals, Zn Sn, investigated toward photocatalytic hydrogen evolution. The Sn derivative, SnPy3P-FF, able produce photocatalytically in presence of known cobaloxime catalyst TEOA as sacrificial electron donor. When nanostructures SnPy3P-FF were employed system, enhanced production observed....
One of the central problems in protein folding is how amino acid sequences within polypeptide chains direct chain and avoid off-pathway aggregation both intracellular environments test tube. The tailspike phage P22 a model system for which genetic analysis has permitted mutational dissection role positions directing its vivo folding. Two classes mutations that affect have been characterized; temperature-sensitive (tsf) mutants second-site suppressors tsf mutants. Here we report effects these...
Amyloid fibrils are fibrous beta-structures that derive from abnormal folding and assembly of peptides proteins. Despite a wealth structural studies on amyloids, the nature amyloid structure remains elusive; possible connections to natural, beta-structured motifs have been suggested. In this work we focus understanding formation sequences protein. We show short (25 6 amino acids) corresponding repetitive adenovirus fiber shaft an intrinsic capacity form as judged by electron microscopy,...
Abstract A series of conjugates amino acids with porphyrins and corroles was synthesized. Their self‐assembling ability under defined conditions investigated by scanning electron microscopy. The morphology photophysical properties these molecules were studied absorption fluorescence spectroscopy in solid, liquid, self‐assembled forms. We observed that both corrole porphyrin conjugated the l ‐phenylalanine– ‐phenylalanine peptide to form spherical nanostructures bathochromic shifts emission...