A5043659983- Developmental Biology and Gene Regulation
- Congenital heart defects research
- Marine Ecology and Invasive Species
- Marine Biology and Environmental Chemistry
- Proteoglycans and glycosaminoglycans research
- Cell Adhesion Molecules Research
- Language, Discourse, Communication Strategies
- Interpreting and Communication in Healthcare
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Genomics and Phylogenetic Studies
- Microtubule and mitosis dynamics
- Invertebrate Immune Response Mechanisms
- Parasite Biology and Host Interactions
- Physiological and biochemical adaptations
- Ichthyology and Marine Biology
- Hippo pathway signaling and YAP/TAZ
- Palliative Care and End-of-Life Issues
- Linguistic Variation and Morphology
- Migration, Health and Trauma
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Caveolin-1 and cellular processes
- Marine Invertebrate Physiology and Ecology
- Patient-Provider Communication in Healthcare
- Head and Neck Cancer Studies
Swarthmore College
2013-2024
National Institutes of Health
2018
University of Washington
2001-2016
University of Arizona
1995-2012
Janssen Scientific Affairs (United States)
2012
Janssen (United States)
2012
New York Chiropractic College
2012
Northwestern Health Sciences University
2012
University of California, Berkeley
1995-2010
Cellular Research (United States)
2010
The first chordates appear in the fossil record at time of Cambrian explosion, nearly 550 million years ago. modern ascidian tadpole represents a plausible approximation to these ancestral chordates. To illuminate origins chordate and vertebrates, we generated draft protein-coding portion genome most studied ascidian, Ciona intestinalis . contains ∼16,000 genes, similar number other invertebrates, but only half that found vertebrates. Vertebrate gene families are typically simplified form ,...
Increases in immigration have led to an enormous growth the number of cross‐linguistic medical encounters taking place throughout United States. In this article role hospital‐based interpreters cross‐linguistic, internal medicine ‘medical interviews’ is examined. The interpreter's actions are analyzed against historical and institutional context within which she working, also with eye goals that frame patient‐physician discourse. Interpreters found not be acting as ‘neutral’ machines...
Physician-patient discourse, even same-language may be viewed as a type of cross-cultural interaction. Inside the US, there is large and increasing number adult Third World immigrant patients who do not speak English. For these "cross-cultural" aspect First medical practice underscored by presence interpreter, herself faced with task negotiating both content utterances also cultural contexts from into which are conveyed. Analysis shows that, through variable patterns how when interpreted,...
Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control behavior morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac migration in ascidian Ciona intestinalis . The heart network regulates genes involved most cellular activities required for migration, including adhesion, polarity, membrane protrusions. We demonstrated that fibroblast growth factor...
Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for characterizing complex developmental processes, such as the initial phases of chordate heart development. The basic helix-loop-helix regulatory Ci-Mesp is required activation cardiac transcription factors. Evidence presented that Ci-Ets1/2, transcriptional effector receptor tyrosine kinase (RTK) signaling, acts downstream from Mesp to establish field. Asymmetric Ets1/2, possibly through localized expression...
Heart development requires precise coordination of morphogenetic movements with progressive cell fate specification and differentiation. In ascidian embryos, FGF/MAPK-mediated activation the transcription factor Ets1/2 is required for heart tissue migration. We found that FoxF one first genes to be activated in precursors response FGF signaling. identified minimal enhancer used a cis-trans complementation test show can interact vivo. Next, we function downstream parallel FGF/MAPK/Ets cascade...
ANISEED (https://www.aniseed.cnrs.fr) is the main model organism database for worldwide community of scientists working on tunicates, vertebrate sister-group. Information provided each species includes functionally-annotated gene and transcript models with orthology relationships within echinoderms, cephalochordates vertebrates. Beyond genes system describes other genetic elements, including repeated elements cis-regulatory modules. Gene expression profiles several thousand are formalized in...
Spanish is a pro-drop language in which first and second person subject pronouns are frequently omitted (80%); traditionally the function of overt has been regarded as adding emphasis or to contrast explicit statements. I argue that their more similar topicalized NPs, referred X-Forms by Klein-Andreu (1989a,b), appearance governed conversational discourse topics; they used pragmatically meta-linguistically switch reference, for purposes negotiating turns, add ‘pragmatic weight’ frames...
Here we exploit the extensive cell lineage information and streamlined genome of ascidian, Ciona intestinalis , to investigate heart development in a basal chordate. Several cardiac genes were analyzed, including sole ortholog Drosophila tinman gene, tissue-specific enhancers isolated for some genes. Conserved sequence motifs within these facilitated isolation enhancer Hand-like gene. Altogether, studies provide regulatory framework differentiation mesoderm, beginning at 110-cell stage,...
Ascidian metamorphosis represents a powerful model for comparative work on chordate development that has remained largely unexplored. We isolated transcripts differentially expressed during in the ascidian Boltenia villosa by suppressive PCR subtractions of staged larval and juvenile cDNAs. employed series three to dissect gene expression metamorphosis. have 132 different protein coding sequences, 65 these show significant matches GenBank proteins. Some genes putative functions relevant key...
The bHLH transcription factor Mesp has an essential but ambiguous role in early chordate heart development. Here, we employ the genetic and morphological simplicity of basal Ciona intestinalis to elucidate regulation function. Characterization a minimal cardiac enhancer for gene demonstrated direct activation by T-box Tbx6c. was fused GFP, permitting high-resolution visualization cells as they migrate divide. also used drive targeted expression activator form Mesp, which induces formation...
Aldehyde dehydrogenases (ALDHs) catabolize toxic aldehydes and process the vitamin A-derived retinaldehyde into retinoic acid (RA), a small diffusible molecule pivotal chordate morphogen. In this study, we combine phylogenetic, structural, genomic, developmental gene expression analyses to examine evolutionary origins of ALDH substrate preference. Structural modeling reveals that processing aldehydes, such as acetaldehyde, by ALDH2, versus large including retinaldehyde, ALDH1A is associated...
To characterize patterns of communication in the offer long-acting injectable (LAI) antipsychotic medication made by psychiatrists to patients with schizophrenia (1) examining style and content their interaction (2) determining how these may have driven ultimate response recommendations for LAI therapy.This was an observational study conducted at 10 community mental health centers 3 waves from July 2010 May 2011. The final dataset discourse analysis 33 recorded conversations which a...
We show that sequence comparisons at different levels of resolution can efficiently guide functional analyses regulatory regions in the ascidians Ciona savignyi and intestinalis . Sequence alignments several tissue-specific genes guided discovery minimal are active whole-embryo reporter assays. Using Troponin I ( TnI ) locus as a case study, we more refined local then be used to reveal substructure within region. A high-resolution saturation mutagenesis conjunction with comparative defined...