A5046968414- Calcium signaling and nucleotide metabolism
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Immunodeficiency and Autoimmune Disorders
- Mosquito-borne diseases and control
- Immunotherapy and Immune Responses
- Gastrointestinal disorders and treatments
- Toxin Mechanisms and Immunotoxins
- Malaria Research and Control
- Helicobacter pylori-related gastroenterology studies
- COVID-19 Clinical Research Studies
- Viral Infections and Vectors
- NF-κB Signaling Pathways
- Erythrocyte Function and Pathophysiology
- Eosinophilic Esophagitis
- Adenosine and Purinergic Signaling
- vaccines and immunoinformatics approaches
- Immune responses and vaccinations
- PARP inhibition in cancer therapy
- Immunotoxicology and immune responses
- Mycobacterium research and diagnosis
- Lung Cancer Research Studies
- Atherosclerosis and Cardiovascular Diseases
- Viral Infections and Outbreaks Research
Instituto Politécnico Nacional
2005-2024
Center for Research and Advanced Studies of the National Polytechnic Institute
2011-2024
Centro de Investigación en Materiales Avanzados
2001-2022
Universidad Veracruzana
2018
CD38 is a transmembrane protein expressed in B lymphocytes, and able to induce responses as proliferation, differentiation or apoptosis. Several reports propose that deficiency accelerates autoimmune processes murine models of diabetes, lymphoproliferation rheumatoid arthritis. Other have shown elevated expression T cells from patients with autoimmunity; however, the role still unclear development autoimmunity. Recently, it has been characterized CD1dhi CD5+ regulatory cell subpopulation...
Cross-linking of CD38 on hematopoietic cells induces activation, proliferation and differentiation mature T B mediates apoptosis myeloid lymphoid progenitor cells. In addition to acting as a signaling receptor, is also an enzyme capable producing several calcium-mobilizing metabolites, including cyclic adenosine diphosphate ribose (cADPR). It has been previously postulated that the metabolites produced by may regulate its receptor-based activities. To test this hypothesis, we examined...
CD38 is a 45,000 molecular weight transmembrane protein that expressed in immature and mature lymphocytes. However, the expression function of during B-cell differentiation mice poorly understood. Here, we report from earliest stages development. Pre-pro-B, pro-B, pre-B B cells murine bone marrow all stained positive for CD38. Interestingly, increases with maturation. To assess role maturation, CD38-deficient were analysed. CD38(-/-) showed significant increase both frequency B-lineage...
Colostrum is the primary source of maternal immunoglobulin A (IgA) for newborn. IgA participates in protection and regulation mechanisms immune response at neonate's mucosa. Several studies have evaluated infectious diseases vaccine protocols effects during pregnancy on milk levels, with aim to understand lactation protecting effect However, most their results demonstrated that there were no differences total levels. In humans, has two subclasses (IgA1 IgA2), they an anatomical distribution...
Abstract Cell spreading and phagocytosis are notably regulated by small GTPases GAP proteins. TBC1D10C is a dual inhibitory protein with activity. In immune cells, one of the elements regulating lymphocyte activation. However, its specific role in macrophages remains unknown. Here, we show that engages functions dependent on cytoskeleton plasma membrane reorganization. Using ex vivo vitro assays, found elimination overexpression modified cytoskeletal architecture decreasing increasing...
Naringenin is flavonoid mainly found in citrus fruits which has shown several biological properties. In this work, we evaluated the therapeutic potential of Naringenin. Five-month-old B6.MRL-Faslpr/J lupus-prone mice were administered daily orally with for seven months. We showed that treatment at 50 or 100 mg/kg inhibited splenomegaly and decreased levels anti-nuclear anti-dsDNA autoantibodies. Furthermore, a reduction serum concentration TNF-α, IFN-γ IL-6 was observed provided...
During the COVID-19 pandemic, identifying reliable biomarkers for predicting disease severity and patient outcomes in unvaccinated individuals is essential. This study evaluates efficacy of key hematological markers, including leukocyte neutrophil counts, Neutrophil-to-Lymphocyte Ratio (NLR), cytokine profiles (IL-6, INF-γ, TNF-α, IL-17A, CCL2, CXCL10) necessity mechanical ventilation assessing survival probabilities.
Dengue virus (DENV) RNA replication requires 2 viral proteins, non-structural protein 3 (NS3) and NS5. NS5 consists of functional domains: a methyltransferase (MTase) domain involved in cap formation located the amino terminal region RNA-dependent polymerase essential for carboxyl region. To gain additional insight into structural interactions between proteins cellular factors DENV replication, we generated panel rat monoclonal antibodies (mAbs) against MTase domain. Six mAbs were selected...
The suppressor effect of T regulatory lymphocytes in co-cultures with effector cells obtained by magnetic columns from healthy donors and activated CD3/CD28 was measured a proliferation assay using BrdU incorporation an ELISA test. Tritiated thymidine used as reference since it is the gold standard for assays. Both methods were simultaneously same samples order to compare them. Correlation between them statistically significant (p < 0.001). purification very efficient CD4+CD25+ also FOXP3+...
CD40, a glycoprotein expressed on B lymphocytes plays an important role in cell development, growth and differentiation. The ligand for the CD40 is 39-kDa (CD154) onthe surface of activated T essential thymus-dependent humoral immunity. expression CD154 tightly regulated its transient reduces chances potentially deleterious bystander activation cells. Stimulation through has been studied vitro by using antibodies against membranes cells or lately, transfected In this work we have evaluated...
CD38 is a transmembrane glycoprotein expressed by T-cells. It has been reported that patients with systemic lupus erythematosus (SLE) showed increased CD38+CD25+ T-cells correlating immune activation and clinical signs. Contrariwise, deficiency in murine models shown enhanced autoimmunity development. Recent studies have suggested CD38+ regulatory are more suppressive than CD38- Thus, we overexpression SLE could play role regulating cells instead of enhancing it. This study found correlation...
Dengue is considered a reemerging disease of worldwide distribution. The virus non-structural protein 3 (NS3) known to possess ATPase, helicase, and protease activities that are constitutive part the replication complex virus. In this report, we discuss cloning, expressing, purifying Dengue-2 NS3 protein, immunize mice then generate monoclonal antibodies (MAbs). Our results show production MAbs specific virus, which by immunofluorescence recognize native in experimentally infected...
Dengue viruses ( DENVs ; serotypes 1–4) are members of the flavivirus family. The envelope protein E ) DENV has been defined as principal antigenic target in terms protection and diagnosis. Antibodies that can reliably detect surface glycoprotein necessary for describing mapping new epitopes well developing more reliable inexpensive diagnostic assays. In this study, we describe production characterization a monoclonal antibody (mAb) against recombinant ‐2 recognizes sequential antigen both...
Lrba is a cytoplasmic protein involved in vesicular trafficking. Lrba-deficient (Lrba-/-) mice exhibit substantially higher levels of IgA both serum and feces than wild-type (WT) mice. Transforming growth factor β1 (TGFβ1) its receptors (TGFβR I II) essential for differentiating IgA+ B cells. Furthermore, increased production suggests potential connection between the TGFβR signaling pathway production. However, specific function cell biology remains unknown. Given Lrba-/- mice, goal this...
Introduction Lipopolysaccharide-responsive and beige-like anchor (LRBA) is a scaffolding protein that interacts with proteins such as CTLA-4 PKA, the importance of which has been determined in various cell types, including T regulatory cells, B renal cells. LRBA deficiency associated an inborn error immunity characterized by immunodeficiency autoimmunity. In addition to defects patients also exhibit defects, reduced number, low memory hypogammaglobulinemia, impaired proliferation, increased...
Abstract Colostrum, the first milk secretion, is characterized by a high concentration of immunologically active molecules with neutralizing and antibacterial capabilities. Mycobacterium tuberculosis microorganism primarily infecting lungs but can also affect non-pulmonary tissues. Despite BCG vaccination coverage exceeding 97% in Mexico, pulmonary meningeal cases have shown yearly increase. This study aimed to determine presence IgA antibodies against soluble extracts from H37Rv M. antigens...
Abstract Lipopolysaccharide-Responsive Beige-like anchor (LRBA) is a scaffolding protein whose deficiency associated with an inborn error of immunity like common variable immunodeficiency. Patients LRBA have heterogeneous clinical presentation including hypogammaglobulinemia, reduction B and T regulatory cells, recurrent infections, autoimmune diseases. Functions Lrba on cells been described, however, function remains unknown. This work aims to identify the in B-cell receptor (BCR)...
The current pandemic generated by SARS-CoV-2 has led to mass vaccination with different biologics that have shown wide variations among human populations according the origin and formulation of vaccine. Studies evaluating response in individuals a natural infection before been limited antibody titer analysis few humoral cellular markers, showing more rapid intense than without prior infection. However, basis these differences not explored depth. In present work, we analyzed group pro...
A novel cell population denominated IFN-γ-producing killer dendritic cells (IKDCs) have been recently described. These are lymphocytes lacking B- or T- receptors, but they can be identified by the presence of B220+ CD38+ CD49b+ and low CD11c, among other surface markers. The main characteristics IKDCs production IFN-γ ability to spontaneously kill tumor cells. We found that this increases in B6.MLR-Faslpr /J mice. Interestingly, increase with age more abundant mice older than 6 months...
Mongolian gerbils that are experimentally infected with Helicobacter pylori develop a chronic inflammation is similar to natural infections in humans. The aim of this study was compare the antigens H. cagPAI+ and cagPAI- strains expressed during Meriones unguiculatus colonization. We identified strain via Western blotting samples from were subjected unique, mixed, sequential bacterial infections. J99/CG3 (cagPAI+) had lower molecular weight than 251F/CG3 (cagPAI-) strain. There fewer single...
Helicobacter pylori is a Gram-negative bacterium found on the luminal surface of gastric mucosa in at least 50% world's human population. The protective effect breastfeeding against H. infection has been extensively reported; however, mechanisms behind this protection remain poorly understood. Human IgA from colostrum reactivity antigens. Despite that IgA1 and IgA2 display structural functional differences, their had not previously determined. We attested titers colostrum-IgA subclasses by...
Anton Van Leeuwenhoek fue la primera persona en ob- servar y describir una célula viva a través de microsco- pios que constaban un lente ocular con capacidad realizar hasta 250 aumentos (Backer & Robertson, 2016). Gracias genialidad Leeuwenhoek, hoy sabemos los seres vivos somos organismos consti- tuidos por diferentes tipos celulares funciones es- pecíficas trabajan conjunto para mantener homeostasis. Sin embargo, ello no podría conocerse sin las herramientas nos permitan observar,...
PfTBP is a transcriptional factor required by all three types of RNA polymerases in eukaryotic cells. In order to obtain specific monoclonal antibody (MAb) against PfTBP, DNA fragment 684 base pairs (bp) that contained the complete gene was amplified polymerase chain reaction (PCR) and inserted into pGEX prokaryotic expression vector. The recombinant protein (GST-PfTBP) expressed Escherichia coli, purified, used as antigen immunize mice. MAbs were obtained hybridomas screened enzyme-linked...