A5077256685- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Genetic factors in colorectal cancer
- Drug Transport and Resistance Mechanisms
- Bacterial Identification and Susceptibility Testing
- Sarcoma Diagnosis and Treatment
- TGF-β signaling in diseases
- Diphtheria, Corynebacterium, and Tetanus
- Mycobacterium research and diagnosis
- Cancer Cells and Metastasis
University of Oxford
2024
Radboud University Medical Center
2018-2019
Johns Hopkins University
2019
Radboud University Nijmegen
2018-2019
Radboud Institute for Molecular Life Sciences
2019
Johns Hopkins Medicine
2019
Pro-carcinogenic bacteria have the potential to initiate and/or promote human colon cancer. However, mechanism(s) by which a bacterium may trigger carcinogenesis remains unclear. Herein, we show Bacteroides fragilis toxin (BFT) secretion pathobiont enterotoxigenic (ETBF) triggers in Min Apc /- mice ETBF pro- carcinogenic inflammatory cascade requiring IL-17R, NF-κB, and Stat3 signaling colonic epithelial cells (CEC). This promotes chemokine-dependent recruitment of pro-tumoral myeloid cells....
Objective The human intestinal microbiome plays an important role in inflammatory bowel disease (IBD) and colorectal cancer (CRC) development. One of the first discovered bacterial mediators involves Bacteroides fragilis toxin (BFT, also named as fragilysin), a metalloprotease encoded by enterotoxigenic (ETBF) that causes barrier disruption inflammation colon, leads to tumorigenesis susceptible mice, is enriched mucosa IBD CRC patients. Thus, targeted inhibition BFT may benefit ETBF carrying...
Abstract TGF-β ligand activation suppresses cell growth yet can paradoxically and potently promote cancer invasion metastasis depending on downstream pathway mutational context. Here, we evaluated the basis of this observation in conditional murine intestinal adenoma models with without loss Mothers against decapentaplegic homolog 4 ( Smad4 ), aim identifying TGF-β-BMP-SMAD4 dependent gene expression biomarkers for translational application. Conditional Lgr5 -CreER T2 Apc fl/fl resulted...
11538 Background: Missense variants associated with high frequency, clinical context, splicing, penetrance and biochemical function are scored as pathogenic by ClinVar/ACMG (scores >99%/>90%). Pathogenic scores that range from this cut-point to 10% (benign) referred of unknown significance (VUS). Alphafold2 is a deep neural network algorithm for protein structure trained on known molecular structures. It has informed AlphaMissense (AM) where the impact every possible single missense...