A5074819808- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Colorectal Cancer Treatments and Studies
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Gastrointestinal motility and disorders
- Mycobacterium research and diagnosis
- Colorectal and Anal Carcinomas
- Bacterial Identification and Susceptibility Testing
- Prostate Cancer Diagnosis and Treatment
- Advanced Radiotherapy Techniques
- Medical Imaging Techniques and Applications
- Glioma Diagnosis and Treatment
- Prostate Cancer Treatment and Research
- Helicobacter pylori-related gastroenterology studies
- Vibrio bacteria research studies
- Global Cancer Incidence and Screening
- Pharmacological Effects of Natural Compounds
- Antimicrobial Resistance in Staphylococcus
- Peripheral Nerve Disorders
- Antibiotic Resistance in Bacteria
- Colorectal Cancer Screening and Detection
- Aquaculture disease management and microbiota
- Antifungal resistance and susceptibility
- Cytomegalovirus and herpesvirus research
Memorial Sloan Kettering Cancer Center
2025
University of California, Los Angeles
2002-2024
Johns Hopkins University
1986-2023
Johns Hopkins Medicine
1986-2023
New York State Department of Health
2020-2022
Wadsworth Center
2020-2022
University of Michigan
2002-2015
National Institutes of Health
2010-2015
Olive View-UCLA Medical Center
2014
Sidney Kimmel Comprehensive Cancer Center
2011
Defining the complex role of microbiome in colorectal cancer and discovery novel, protumorigenic microbes are areas active investigation. In present study, culturing reassociation experiments revealed that toxigenic strains Clostridioides difficile drove tumorigenic phenotype a subset patient-derived mucosal slurries germ-free ApcMin/+ mice. Tumorigenesis was dependent on C. toxin TcdB associated with induction Wnt signaling, reactive oxygen species, immune responses marked by infiltration...
PURPOSE: The goal of three-dimensional (3-D) conformal radiation is to increase the dose delivered tumor while minimizing surrounding normal brain. Previously it has been shown that even escalated doses 70 80 Gy have failure patterns are predominantly local. This article describes and survival seen with high-grade gliomas given 90 using a 3-D intensity-modulated technique. PATIENTS AND METHODS: From April 1996 1999, 34 patients supratentorial were treated Gy. For those recurred, defined in...
PURPOSE: The goal of three-dimensional (3-D) conformal radiation is to increase the dose delivered tumor while minimizing surrounding normal brain. Previously it has been shown that even escalated doses 70 80 Gy have failure patterns are predominantly local. This article describes and survival seen with high-grade gliomas given 90 using a 3-D intensity-modulated technique. PATIENTS AND METHODS: From April 1996 1999, 34 patients supratentorial were treated Gy. For those recurred, defined in...
Neutrophils form the first line of host defense against bacterial pathogens. They are rapidly mobilized to sites infection where they help marshal defenses and remove bacteria by phagocytosis. While splenic neutrophils promote marginal zone B cell antibody production in response administered T independent antigens, whether shape humoral immunity other lymphoid organs is controversial. Here we investigate neutrophil influx following local injection Staphylococcus aureus adjacent inguinal...
Enterotoxigenic Bacteroides fragilis (ETBF), a molecular subclass of the common human commensal, B. fragilis, has been associated with inflammatory bowel disease. ETBF colitis is characterized by activation Stat3 and Th17 immune response in colonic mucosa. This study was designed to investigate time course cellular distribution ETBF-colonized mice.
Colorectal cancer is multifaceted, with subtypes defined by genetic, histologic, and immunologic features that are potentially influenced inflammation, mutagens, and/or microbiota. cancers activating mutations in BRAF associated distinct clinical characteristics, although the pathogenesis not well understood. The Wnt-driven multiple intestinal neoplasia (MinApcΔ716/+) enterotoxigenic Bacteroides fragilis (ETBF) murine model characterized IL17-dependent, distal colon adenomas. Herein, we...
Using next-generation sequencing (NGS), we developed and validated a whole-genome (WGS)-based clinical test for fungal species identification on isolates. The is mainly based the ribosomal internal transcribed spacer (ITS) region as primary marker, additional marker genomic analysis applied within Mucorales family (using 28S rRNA gene) Aspergillus genus beta-tubulin gene k-mer tree-based phylogenetic clustering). validation study involving 74 unique isolates (22 yeasts, 51 molds, 1...
Urinary tract infections are prone to overdiagnosis, and reflex urine culture protocols offer a valuable opportunity for diagnostic stewardship in this arena. However, there is no recommended standard testing approach. Cancer patients often excluded from protocols, especially if severely immunosuppressed or neutropenic. The aim of study was evaluate the performance characteristics screening studies, including dipstick urinalysis nitrite leukocyte esterase microscopy white blood cell count,...
Polysaccharide A (PSA), an immunogenic capsular component of non-toxigenic Bacteroides fragilis (NTBF) strain NCTC 9343, is reported to promote mucosal immune development and suppress colitis. Contrastingly, enterotoxigenic (ETBF) highly associated with inflammatory bowel disease (IBD) colorectal cancer (CRC), rapidly inducing IL-17-dependent murine colitis tumorigenesis. In specific-pathogen-free (SPF) C57BL/6 wild-type (WT) multiple intestinal neoplasia (MinApc716+/-) mice, we show that...
ABSTRACT Tetracycline, clindamycin, and other protein synthesis inhibitors at subinhibitory concentrations significantly increased the expression of pivotal virulence regulator agr production -regulated cytolytic phenol-soluble modulins in community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) strain USA300. Our results suggest that such may exacerbate progression CA-MRSA disease when applied are too low or treating infections caused by strains resistant to those antibiotics.
Introduction of a rapid methicillin-resistant Staphylococcus aureus (MRSA) polymerase chain reaction assay, with physician education and pharmacist guidance, did not significantly reduce excessive empiric prescription MRSA-active antibiotics despite the test's accuracy potential to substantially inappropriate antibiotic use.
Hypothyroxinemia and hypertriiodothyroninemia may occur in the course of antithyroid drug or 131I treatment for hyperthyroid Graves' disease. To determine frequency combined high serum T3 low T4 concentrations during such to assess clinical significance its recognition, we reviewed 60 patients treated disease with drugs (n = 43) radioactive iodine 17). Six these (10%) were found have therapy. Four receiving drugs, 2 had received iodine. At time this abnormality occurred, 4 euthyroid, 1 was...
A 39-year-old man presented with a history of fatigue, malaise, and rash varied morphology on his perianal region. Polymerase chain reaction testing the lesions confirmed coinfection monkeypox herpes simplex virus type 2. We emphasize difficulty in distinguishing between 2 based examination alone.
Pro-carcinogenic bacteria have the potential to initiate and/or promote human colon cancer. However, mechanism(s) by which a bacterium may trigger carcinogenesis remains unclear. Herein, we show Bacteroides fragilis toxin (BFT) secretion pathobiont enterotoxigenic (ETBF) triggers in Min Apc /- mice ETBF pro- carcinogenic inflammatory cascade requiring IL-17R, NF-κB, and Stat3 signaling colonic epithelial cells (CEC). This promotes chemokine-dependent recruitment of pro-tumoral myeloid cells....
Human infection caused by bacteria of the Edwardsiella genus is rare and most often presents with gastroenteritis that rarely requires antibiotics. Our case report describes a medically complex patient chronic steroid use contributing to an immunocompromised state, who presented fever abdominal pain. The was later found have tarda (E. tarda) bacteremia underwent paracentesis confirming E. bacterial peritonitis requiring prolonged antibiotic course. This aims illustrate presentation,...
Passive reporting to the Centers for Disease Control and Prevention has identified carbapenemase-producing organisms (CPOs) among solid organ transplant (SOT) recipients, potentially representing an emerging source of spread. We analyzed CPO prevalence in wards where SOT recipients receive inpatient care inform public health action prevent transmission.
Bacterial commensals of the human genitourinary tract, Mycoplasma hominis and Ureaplasma species (parvum urealyticum) can be sexually transmitted, may cause nongonococcal urethritis, pelvic inflammatory disease, infertility. also severe invasive infections in immunocompromised patients. Current culture-based methods for Mycoplasma/Ureaplasma identification are costly laborious, with a turnaround time between 1 2 weeks. We developed high-throughput, real-time multiplex PCR assay rapid...