Background Bacterial infections are usually suspected in infertile couples seeking IVF with no clear understanding of the microbial compositions present seminal fluids and vaginal niche patients. We used next-generation sequencing technology to correlate microbiota clinical outcomes. Methods Thirty-six were recruited provide swabs. DNA was extracted, V4 region 16S rRNA amplified sequenced a pair-end configuration on Illumina MiSeq platform rendering 2 × 150 bp sequences. Microbial taxonomy...

Introduction: This study aimed to identify socio-clinical and immuno-inflammatory markers of early-onset colorectal cancer (CRC) for early detection aggressive in Southern Nigeria. Material Methods: included 89 patients with CRC diagnosed from Jan. 2016 Dec. 2022. The were sub-grouped based on age chemotherapy response. neutrophil-tolymphocyte ratio (NLR), lymphocyte-to-monocyte (LMR), platelet-to-lymphocyte (PLR), platelets-neutrophils-to-lymphocytes (PNLR), neutrophils-to-lymphocytes...

Abstract Background Clinical data exist that support the utility of topical probiotics for certain dermatological diseases such as atopic dermatitis, acne, and psoriasis. However, there is paucity on use live lactobacilli to control axillary malodor. The objective this study was determine whether application oil‐based cream containing Lactobacilli could decrease malodor‐producing bacteria in axilla healthy subjects. Aims To effects with Patients/Methods Twenty‐five adult volunteers...

Summary Tuberculosis (TB) causes significant morbidity and mortality on a global scale. The African region has 24% of the world's TB cases. overlaps with other infectious diseases such as malaria HIV, which are also highly prevalent in region. is leading cause death among HIV-positive patients co-infection HIV been described syndemic. In view overlapping epidemiology these diseases, it important to understand dynamics immune response context co-infection. We investigated cytokine purified...

Against the background of genital mycoplasmas being implicated as pathogens in tracts adult humans, this study was carried out to determine prevalence among women reproductive age attending a tertiary-care hospital Nigeria.One hundred and sixty-eight high vaginal specimens from 114 pregnant 54 non-pregnant with or without complaints were studied for presence using selective bacteriological culture techniques. Isolates identified biochemically serologically.Sixty (35.7 %) isolates obtained...

There is a paucity of information on the oral microbiome compositions Nigerians, mostly due to lack appropriate molecular techniques. In this pilot study, we sought determine and characterize bacterial "healthy" females.Oral samples were collected from three randomly selected females aged 56, 28, 8 years. DNA was extracted 16S rRNA V4 region amplified using custom-barcoded primers before sequencing with Illumina MiSeq platform. Quantitative Insights into Microbial Ecology pipeline used for...

Hyperglycaemic complication is the most common cause of hospitalization amongst diabetes patients in Nigeria. Research showed that self-management education (DSME) assists controlling hyperglycaemia patients. We assessed opinions practice nurses and dieticians on implementing DSME a Nigerian population. 517 completed self-administered questionnaire tool. Results majority participants agreed population will assist (88.3%) to reduce complications (91.4%). While only 34% all believed their...

This study was designed to define the correlation pattern ofCD4/CD8 ratio, inflammatory cytokines, oxidative stress parameters, and cytolytic molecules in chronic HIV infection, using seropositive participants visiting clinic at two hospitals Edo central, Nigeria. A total of 58 sero-positive on HAART 30 apparently healthy individuals (control) were recruited for this study. The CDC staging method with CD4 count used classify participants. average duration ART intake 9.0±3.33 years 8.18±4.19...

This study was aimed to isolate and identify Pseudomonas aeruginosa determine the prevalence rate of isolated P. in Hospitals Onitsha. Isolates were recovered from both clinical environmental sources using Cetrimide agar, Blood Mueller-Hinton agar MacConkey agar. All inoculated plates incubated at 37°C for 24-48 hours growth evaluated on these media. identified basis standard bacteriological methods like morphology, colonial characteristics, smell culture, haemolysis, as well pigment...

Background: Genetic evidence of asymptomatic Mycoplasma hominis (M. hominis) and Ureaplasma urealyticum (U. urealyticum) infection associated with infertility among females is lacking because suitable high throughput molecular methods have not been applied. Objective: This study aimed to explore the occurrence M. U. in genital tract as well determine their genetic relatedness. Materials Methods: The group included 100 31 diagnosed infertility. Sequencing 16S rRNA gene following DNA...

Background: Extended Spectrum Beta Lactamase (ESBL) producing bacterial strains are the major causes of nosocomial and community-acquired infections worldwide.The aim study was to evaluate effectiveness Brilliance ESBL Agar (BEA) (a chromogenic culture medium) for detection in comparison with Double Disc Synergy Test (DDST) confirm results from both methods by Single-plex Polymerase Chain Reaction (PCR) as gold standard.Materials Methods: A total 75 clinical isolates Escherichia coli were...

Ciprofloxacin is an inexpensive antibacterial, whereas chloroquine antimalarial. The coadministration of and ciprofloxacin easily encountered because both drugs are commonly prescribed to patients in the tropics. Five healthy male volunteers aged 19 31 years who were not taking any medications had no sensitivity either or each received 500 mg orally with 250 mL water, after a 2-week washout period, plus 600 was administered water providing informed consent. A urine sample (7 mL) collected...

Here, we aimed to investigate the predominance of cervicitis agents with minimal testing rates among asymptomatic students in a Tertiary Institution and its associated risk factors. Endocervical swabs were collected from randomly selected 133 female (15-34 years age) Benin City, Nigeria screened for six genital pathogens (Chlamydia trachomatis, Mycoplasma hominis, M. genitalium, Ureaplasma urealyticum, U. parvum Trichomonas vaginalis) using multiplex PCR. Out subjects, 80 positive 46.3%...

Background: Bacterial vaginosis (BV) in pregnant women remains a cause for clinical concern among clinicians and health care professionals. BV has been linked to prenatal, antenatal postnatal challenges women. Information on prevalence of across trimesters pregnancy is expected give better insight into the pathophysiology this polymicrobial disorder. This study was conducted determine attending Nnamdi Azikiwe University Teaching Hospital (NAUTH), Nnewi, Nigeria.
 Methodology:...

Background/Objectives: Occult Hepatitis B virus infection (OBI), characterized by presence of HBV DNA in the absence HBsAg individuals has become a challenge to blood safety. The aim this study is determine prevalence rate, serologic markers, viral load and genotypes occult Virus among donors attending Nnamdi Azikiwe University Teaching Hospital Abuja Abuja, Nigeria. Materials/Methods: Sera from 212 healthy (108 104 UATH NAUTH respectively) were retested for HBsAg, HIV, Syphilis antibodies...

Background: This cross-sectional study investigated the serological profile, sociodemographic characters and risk factors of hepatitis B virus (HBV) infection among HBV surface antigen (HBsAg)-negative blood donors at University Abuja Teaching Hospital (UATH) Gwagwalada Nnamdi Azikiwe (NAUTH) Nnewi, Nigeria.Methods: Blood samples collected from 176 HBsAg-negative (96 UATH 80 NAUTH) were screened using a commercially available 5-Panel (CTK Biotech, USA) anti-HBc IgM ELISA kits.Determination...