A5056158867- Bacterial biofilms and quorum sensing
- Immune cells in cancer
- Esophageal and GI Pathology
- Antimicrobial Peptides and Activities
- Congenital Anomalies and Fetal Surgery
- Tracheal and airway disorders
- Intestinal Malrotation and Obstruction Disorders
- Cystic Fibrosis Research Advances
- Urological Disorders and Treatments
- Pediatric Urology and Nephrology Studies
- Immunotherapy and Immune Responses
- 3D Printing in Biomedical Research
- Inhalation and Respiratory Drug Delivery
- Polymer Surface Interaction Studies
- Foreign Body Medical Cases
- Graphene and Nanomaterials Applications
- Immune Response and Inflammation
- Plant tissue culture and regeneration
- Antimicrobial agents and applications
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Congenital Diaphragmatic Hernia Studies
- Congenital gastrointestinal and neural anomalies
- Inflammasome and immune disorders
- Kidney Stones and Urolithiasis Treatments
Parul University
2024
Shree Krishna Hospital
2023
Institute of Medical Sciences
2023
Lala Lajpat Rai Memorial Medical College
2023
University of Nottingham
2011-2023
Queen's Medical Centre
1998-2023
V.I. Vernadsky Crimean Federal University
2023
Ganesh Shankar Vidyarthi Memorial Medical College
2023
University of Ghana
2023
Dubai Medical College
2023
Adverse immune reactions prevent clinical translation of numerous implantable devices and materials. Although inflammation is an essential part tissue regeneration, chronic ultimately leads to implant failure. In particular, macrophage polarity steers the microenvironment toward or wound healing via induction M1 M2 macrophages, respectively. Here, this paper demonstrates that within biomaterials can be controlled through integrin-mediated interactions between human monocytic THP-1 cells...
Macrophages are innate immune cells that have a central role in combating infection and maintaining tissue homeostasis. They exhibit remarkable plasticity response to environmental cues. At either end of broad activation spectrum pro-inflammatory (M1) anti-inflammatory (M2) macrophages with distinct functional phenotypical characteristics. also play crucial orchestrating responses biomaterials used the fabrication implantable devices drug delivery systems. To assess impact different surface...
Antigen presenting cells (APCs) such as macrophages and dendritic (DCs) play a crucial role in orchestrating immune responses against foreign materials. The activation status of APCs can determine the outcome an response following implantation synthetic materials, towards either healing or inflammation. A large range biomaterials are used fabrication implantable devices drug delivery systems. These materials will be close contact with characteristics surface chemistry topography may have...
The mannose receptor (MR) is an endocytic type I membrane molecule with a broad ligand specificity that involved in both hemostasis and pathogen recognition. Membrane-anchored MR cleaved by metalloproteinase into functional soluble (sMR) composed of the extracellular domains intact MR. Although sMR production was initially considered constitutive process, enhanced shedding has been observed response to fungal Pneumocystis carinii. In this work, we have investigated mechanism mediating fungi....
Macrophages are master regulators of immune responses toward implanted biomaterials. The activation state adopted by macrophages in response to biomaterials determines their own phenotype and function as well those other resident infiltrating nonimmune cells the area. A wide spectrum macrophage states exists, with M1 (pro-inflammatory) M2 (anti-inflammatory) representing either ends spectrum. In research, cell-instructive surfaces that favor or induce have been considered beneficial due...
Abstract Bacterial biofilms represent a challenge to the healthcare system because of their resilience against antimicrobials and immune attack. Biofilms consist bacterial aggregates embedded in an extracellular polymeric substance (EPS) composed polysaccharides, nucleic acids proteins. We hypothesised that carbohydrates could contribute recognition Pseudomonas aeruginosa by engaging C-type lectins. Here we show binding Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing...
This study aims to design an optimal polyelectrolyte multilayer film of poly-l-lysine (PLL) and hyaluronic acid (HA) as anti-inflammatory cytokine release system in order decrease the implant failure due any immune reactions. The chemical modification HA with aldehyde moieties allows self-cross-linking improvement mechanical properties film. cross-linking immunomodulatory (IL-4) stimulate differentiation primary human monocytes seeded on films into pro-healing macrophages phenotype. induces...
The rising occurrence of antimicrobial resistance demands new strategies for delivering antibiotics to ensure their effective use. In this study, a multi-functional strategy address medical device associated infections is explored whereby an anti-attachment and antibacterial mechanism have been combined. Silicone catheters impregnated with multiple are coated polyacrylate coatings previously shown reduce bacterial attachment biofilm formation. Antibiotics delivered through the applied...
Summary Tuberculosis (TB) causes significant morbidity and mortality on a global scale. The African region has 24% of the world's TB cases. overlaps with other infectious diseases such as malaria HIV, which are also highly prevalent in region. is leading cause death among HIV-positive patients co-infection HIV been described syndemic. In view overlapping epidemiology these diseases, it important to understand dynamics immune response context co-infection. We investigated cytokine purified...
Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe infections at compromised epithelial surfaces, such those found in burns, wounds, and lungs damaged by mechanical ventilation or recurrent infections, particularly cystic fibrosis (CF) patients. CF patients have been proposed to a Th2 Th17-biased immune response suggesting the lack of Th1 and/or over exuberant Th17 responses could contribute establishment chronic P. infection deterioration lung function. Accordingly,...
Pseudomonas aeruginosa causes infections in patients with compromised epithelial barrier function. Multiple virulence factors produced by P. are controlled quorum sensing (QS) via 2-alkyl-4(1H)-quinolone (AQ) signal molecules. Here, we investigated the impact of AQs on PAO1 infection differentiated human bronchial cells (HBECs). The pqsA-E operon is responsible for biosynthesis including 2-alkyl-3-hydroxy-4-quinolones, 4-hydroxy-2-alkylquinolines, and 4-hydroxy-2-alkylquinoline N-oxides as...
To report a case of misdiagnosis an impacted oesophageal button battery in child, and to describe the associated risk factors for impaction management such cases.An 18-month-old, otherwise fit well child with stridulous respiration was initially treated croup. Medical treatment over course three months failed, appropriate imaging subsequently demonstrated upper oesophagus. This promptly removed. There were no signs damage on direct visualisation, or follow-up contrast swallow image.This...
Production of secondary metabolites from plant tissue culture has emerged as a promising and feasible option attracting the attention scientists worldwide. Plant cell, organ cultures offer an attractive alternative for homogeneous, controlled production metabolites, throughout year, especially when we take commercial demand into account. They not only facilitate de novo synthesis novel compounds, but also are able to produce compounds sometimes even in higher amounts than intact plants. Many...
Adverse immune reactions to implanted devices can seriously hamper the efficacy of implants. Monocyte derived macrophages play a crucial role in both initiation and resolution inflammatory response toward foreign bodies. As surface microtopography is shown exert significant effects on cell phenotype, it hypothesized that presence micropatterns implant/medical device surfaces attenuate response. To this end, enzymatically crosslinked micropatterned gelatin films varying groove widths (2, 5,...
Macrophages are important orchestrators of inflammation during bacterial infection, acting as both effector cells and regulators neutrophil recruitment life span. Differently activated macrophage populations with distinct inflammatory microbicidal potentials have been described. Our previous work unveiled a positive negative correlation between levels gamma interferon (IFN-γ) interleukin-17A (IL-17A), respectively, lung function in cystic fibrosis, particularly patients chronically infected...
Tumour necrosis factor receptor-associated periodic syndrome (TRAPS) is a hereditary autoinflammatory disorder characterized by recurrent episodes of fever and inflammation. It associated with autosomal dominant mutations in TNFRSF1A, which encodes tumour receptor 1 (TNF-R1). Our aim was to understand the influence TRAPS on response stimulation pattern recognition Toll-like (TLR)-9. Peripheral blood mononuclear cells (PBMCs) serum were isolated from patients healthy controls: levels 15...