The aim of this study was to analyze data on gabapentinoid-related attendances the National Poison Control Center Serbia (NPCC), particularly abuse cases; estimate its changes and compare it with trends in national consumption rates these drugs. We also aimed main characteristics population investigate major clinical effects poisoned patients.This is a retrospective patients admitted NPCC for acute poisoning involving gabapentinoids from 1 May 2012 October 2022.There were 357 (95.5%)...

Carbamazepine is antiepileptic drug widely used for the treatment of epilepsy. Due to low therapeutic index carbamazepine there a need routine measuring its concentrations in biological fluids. The aim study was describe method concomitant determination serum and saliva.Separation from matrix achieved by reversed-phase chromatography on C18 column, with mobile phase methanol-water-acetic acid (65:34:1) at flow-rate 1.0 ml/min. Detection effected ultra-violet absorption 285 nm. total run time...

Introduction. ?Body packer? syndrome with severe intoxication or sudden death may happen in persons who smuggle drugs their body cavities. In case of lethal outcome when carrying cocaine, it is important, but sometimes difficult to determine whether was due other causes. Therefore, necessary not only quantify cocaine and its metabolites biological material, also based on distribution fluids tissues conclude acute intoxication. We described a well-documented fatal poisoning packer post mortem...

Saliva is a body fluid which, like serum, can be used for determination of concentrations certain drugs, both in pharmacotherapy as well acute poisonings. The aim this study was to determine carbamazepine saliva and serum poisoning order show if there correlation between the obtained values, monitor toxicokinetics fluides.Saliva samples were from 26 patients treated with 20 acutely poisoned by drug immediately after their admission Emergency Toxicology Unit. Determination salivary performed...

Abstract A rising number of patients are being treated for overdosing with new psychoactive substances (NPS) available at the illegal drug market in Serbia. The aim this study was to report clinical and analytical experience National Poison Control Centre Serbia (NPCC) synthetic cannabinoids (SCs) point NPS our country. From January 2013 December 2016, 58 (aged between 14 25) were effects NPCC. Tachycardia established 53, mydriasis 31, somnolence, nausea, vomiting, agitation 16, dizziness...

Abstract Pregabalin is a gabapentinoid approved for the treatment of general anxiety disorder, neuropathic pain and as adjunctive therapy focal seizures in patients with epilepsy. In addition, there are number conditions which pregabalin prescribed off-label. Along widespread use significant reports describing misuse over last decade. Over time, it became clear that should become part routine testing toxicology laboratories. The aim this paper was to present validation LC-MS/MS method...

Abstract: The aim of the study was to examine antidotal potency trimedoxime in mice poisoned with three direct dimethoxy‐substituted organophosphorus inhibitors. In order assess protective efficacy against dichlorvos, heptenophos or monocrotophos, median effective doses and half‐times were calculated. Trimedoxime (24 mg/kg intravenously) injected 5 min. before 1.3 LD50 intravenously poisons. Activities brain, diaphragmal erythrocyte acetylcholinesterase, as well plasma carboxylesterases...

Background/Aim. Quantitative analysis of amoxicillin and clavulanic acid in biological matrices requires sensitive specific methods which allow determination therapeutic concentration ?g/mL range. Analytical for their concentrations body fluids described literature include high performance liquid chromatography coupled to UV detector (HPLC-UV) chromatography-mass spectrometry (LC-MS). The aim this study was develop ultra chromatography/ mass (UPLC/MS) method could be used the spectral...

Based on numerous studies in animals, the most prominent toxic effects of decabrominated diphenyl ether (BDE-209) are observed liver, thyroid hormone homeostasis, reproductive and nervous systems. BDE-209 exhibits its partly through aryl hydrocarbon (Ah) receptor consequent induction hepatic microsomal enzymes. The aim this study was to assess hepatotoxic effect vs target tissue dose subacutely orally exposed Wistar rats. Effects were examined male rats, weighing 200-240 g, doses 1;000,...

The aim of the study was to evaluate bioequivalence two itraconazole 100 mg capsule formulations.The single-center, open-label, randomized, three-period, three-sequence, reference-replicated, cross-over included 38 healthy subjects under fed conditions. In each period (separated by a 14-day washout), single oral dose test (T) or reference (R) product administered. Blood samples were collected at pre-dose and up 72.0 h after administration. calculated pharmacokinetic parameters, based on...

Background/Aim. Switching the patient from one pharmaceutical formulation of same drug to another, may lead therapeutic inadequancy in some cases. To minimize risk, careful pharmacokinetic studies are desired pre-registration period and afterwards. Methods. A randomized, crossover design with one-week wash-out between each dose was applied. Serum samples, obtained before dosing at various appropriate time points up 15 hours, were analyzed for nimesulide content by a high-performance liquid...

Background/Aim. Zeolites are the hydratised alumosilicates of alcali and earthalcali cations, which have a long three-dimensional crystal structure. Preparations on basis zeolites used for adsorption organic nonorganic toxic substances they, also, find more use in veterinary human medicine pharmacy. The aim this study was to evaluate possibilities zeolite adsorb vitamins B1, B2 B6 acid neutral solutions, as well characteristics process (saturability, reversibility competitivness). Methods....

Wistar rats were treated with T-2 toxin (1 LD50; 0.23 mg/kg sc) and the surviving animals sacrificed on days 1, 3, 5, 7, 14, 21 28 after treatment. At each time, control sacrificed, too. Cardiac mast cells, previously stained by Giemsa method, analyzed in whole visual fields, magnification x40. In present study following quantitative morphometric parameters of cardiac cells evaluated: perimeter (P), area (A) roundness (R). groups majority small (P = 6.86 - 7.99 mm), hypogranular (A 11.60...

Alpha-amanitin is a cyclic peptide which belongs to large group of mushroom toxins known as amatoxins. Being responsible for the majority fatal poisonings, they require rapid detection and excretion from body fluids. In accordance with these requirements, simple an accurate method was developed successful identification quantification alpha-amanitin in serum electrospray liquid chromatography–mass spectrometry (LC-ESI-MS) after collision-induced dissociation. The conforms established...