A5010607661- Gut microbiota and health
- Vector-borne infectious diseases
- Dermatology and Skin Diseases
- Extracellular vesicles in disease
- Psoriasis: Treatment and Pathogenesis
- Clostridium difficile and Clostridium perfringens research
- Trypanosoma species research and implications
- Insect symbiosis and bacterial influences
- Listeria monocytogenes in Food Safety
- Cancer Research and Treatments
- IL-33, ST2, and ILC Pathways
- Vector-Borne Animal Diseases
- Nanoplatforms for cancer theranostics
- Inflammatory Bowel Disease
- Leptospirosis research and findings
- Autophagy in Disease and Therapy
- Cancer Immunotherapy and Biomarkers
- Body Contouring and Surgery
- Vibrio bacteria research studies
- Immune Cell Function and Interaction
- Bacterial Infections and Vaccines
- Medical and Biological Ozone Research
- Plant and Fungal Interactions Research
- Complementary and Alternative Medicine Studies
- Microbial Metabolites in Food Biotechnology
Tufts University
2016-2020
University of Wisconsin–Madison
2019
Tufts Medical Center
2013-2014
Harvard University
2010-2012
Brigham and Women's Hospital
2010-2012
Listeria monocytogenes is an intracellular pathogen that able to colonize the cytosol of macrophages. Here we examined interaction this with autophagy, a host cytosolicdegradative pathway constitutes important component innate immunity towards microbial invaders. L. infection induced activation autophagy system in At 1 h post (p.i.), population bacteria (~37%) colocalized marker LC3. These were within vacuoles and targeted by LLO-dependent manner. later stages (by 4 p.i.), majority escaped...
Borrelia burgdorferi is an invasive spirochete that can cause acute and chronic infections in the skin, heart, joints, central nervous system of infected mammalian hosts. Little understood about where bacteria encounter strongest barriers to infection how different components host immune influence population as progresses. To identify bottlenecks a murine host, we utilized Tn-seq monitor composition mixed populations B. during infection. Both wild-type mice lacking Toll-like receptor adapter...
Borrelia burgdorferi maintains a complex life cycle between tick and vertebrate hosts. Although some genes have been identified as contributing to bacterial adaptation in the different hosts, list is incomplete. In this manuscript, we report first use of transposon mutagenesis combined with high-throughput sequencing (Tn-seq) B. burgdorferi. We utilize technique investigate mechanisms carbohydrate utilization role metabolism during mouse infection. performed genetic fitness analyses identify...
EDP1815 is a non-colonizing pharmaceutical preparation of single stain Prevotella histicola isolated from the duodenum human donor. We report here preclinical and clinical studies showing that action EDP1815, an orally delivered gut restricted strain commensal bacteria can regulate inflammatory responses throughout body.Supported by evidence for anti-inflammatory activity in three mouse models Th1-, TH2-, Th17-mediated inflammation, was tested clinically Phase 1b patients with psoriasis,...
Orientations of the seven invertible polysaccharide biosynthesis locus promoters Bacteroides fragilis were determined from bacteria grown in vitro, feces monoassociated and complex colonized mice, B. fragilis-induced murine abscesses. Bacteria vivo have greater variability orientation than culture-grown organisms.
Post-transcriptional regulation via small regulatory RNAs (sRNAs) has been implicated in diverse processes bacteria, including virulence. One class of sRNAs, termed trans-acting can affect the stability and/or translational efficiency regulated transcripts. In this study, we utilized a collaborative approach that employed data from infection with Borrelia burgdorferi Tn library, coupled Tn-seq, together borrelial sRNA and total RNA transcriptomes, to identify an intergenic sRNA, which...
e14241 Background: Systemic immunity is regulated by interactions of commensal bacteria with immune cells in the gut. Enrichment specific intestinal microbes has been shown to enhance anti-tumor response PD-1 blockade both murine models and cancer patients. Here we report that oral administration a monoclonal microbial, EDP1503, induces systemic immunity. Methods: The mechanism action efficacy EDP1503 was investigated isograft tumor variety ex vivo vitro studies human cells. Results:...
ABSTRACT Post-transcriptional regulation via small regulatory RNAs (sRNAs) has been implicated in diverse processes bacteria, including virulence. One class of sRNAs, termed trans -acting can affect the stability and/or translational efficiency regulated transcripts. In this study, we utilized a collaborative approach that employed data from infection with Borrelia burgdorferi Tn library, coupled Tn-seq, together borrelial sRNA and total RNA transcriptomes, to identify an intergenic sRNA,...
<h3>Background</h3> The small intestinal axis (SINTAX) is a network of anatomic and functional connections between the intestine rest body. It acts as an immunosurveillance system, integrating signals from environment that affect physiological processes throughout impact events in gut control tumor immunity beginning to be appreciated. We have previously shown orally delivered single strain commensal bacteria induces anti-tumor preclinically via pattern recognition receptor-mediated...
Hydropic conditions of the bovine uterus are encountered sporadically by practitioner. Although hydrops is not an everyday occurrence, it important to recognize condition so appropriate medical treatment can be instituted.
Abstract The small intestinal axis (SINTAX) is a network of anatomic and functional connections between the intestine rest body. It acts as an immunosurveillance system, integrating signals from environment that affect physiological processes throughout impact events in gut control tumor immunity beginning to be appreciated. We have previously shown orally delivered single strain commensal bacteria induces anti-tumor via activation innate adaptive immunity. Some produce extracellular...