A5065242571- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- RNA modifications and cancer
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Catalysis and Immobilization
- Explainable Artificial Intelligence (XAI)
- Scientific Computing and Data Management
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Peptidase Inhibition and Analysis
- Biotin and Related Studies
- Climate change impacts on agriculture
- Microbial Metabolic Engineering and Bioproduction
- Click Chemistry and Applications
- Cancer Research and Treatments
- Remote Sensing in Agriculture
- Virus-based gene therapy research
Technical University of Munich
2017-2023
King Abdullah University of Science and Technology
2017-2019
Center for Integrated Protein Science Munich
2017-2019
Klinik und Poliklinik für Urologie
2017
München Klinik
2017
Abstract The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA Pyl are extensively used to add non-canonical amino acids (ncAAs) the genetic code of bacterial eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process generate an appropriate PylRS/tRNA pair. We here report strategy predict PylRS variant with novel properties. designed polyspecific HpRS catalyzes aminoacylation 31 structurally diverse bearing clickable, fluorinated, fluorescent, for...
Enzymes originating from hostile environments offer exceptional stability under industrial conditions and are therefore highly in demand. Using single-cell genome data, we identified the alcohol dehydrogenase (ADH) gene, adh/a1a, Atlantis II Deep Red Sea brine pool. ADH/A1a is active at elevated temperatures high salt concentrations (optima 70 °C 4 m KCl) withstands organic solvents. The polyextremophilic exhibits a broad substrate scope including aliphatic aromatic alcohols able to reduce...
Adenoviral vector production for therapeutic applications is a well-established routine process. However, current methods measurement of adenovirus particle titers as quality characteristic require highly purified virus preparations. While typically obtained in the last step downstream purification, rapid and reliable quantification intermediate products crude lysates to allow optimization validation cell cultures processing steps are currently not at hand. Light scattering an established...
In recent years, artificial intelligence (AI) has deeply impacted various fields, including Earth system sciences. Here, AI improved weather forecasting, model emulation, parameter estimation, and the prediction of extreme events. However, latter comes with specific challenges, such as developing accurate predictors from noisy, heterogeneous limited annotated data. This paper reviews how is being used to analyze events (like floods, droughts, wildfires heatwaves), highlighting importance...
Abstract The biotin–streptavidin interaction is among the strongest known in nature. Herein, site‐directed incorporation of biotin and 2‐iminobiotin composed noncanonical amino acids (ncAAs) into proteins reported. 2‐Iminobiotin lysine was employed for protein purification based on pH‐dependent dissociation constant to streptavidin. By using high‐affinity binding lysine, bacterial RecA could be specifically isolated its partners analyzed. Furthermore, biotinylation approach successfully...
Climate variability and extremes are known to represent major causes for crop yield anomalies. They can lead the reduction of productivity, which results in disruptions food availability nutritional quality, as well rising prices. Extreme climates will become even more severe global warming proceeds, challenging achievement security. These extreme events, especially droughts heat waves, already evident food-production regions like United States. Crops cultivated this country such corn...
Abstract The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA Pyl are used to facilitate the incorporation of non-canonical amino acids (ncAAs) into genetic code bacterial eukaryotic cells by orthogonally reassigning amber codon. Currently, new ncAAs requires a cumbersome engineering process composed several positive negative selection rounds select appropriate PylRS/tRNA pair. Our fast sensitive approach required only single FACS round identify 110 orthogonal PylRS variants for...