A5046898824- Enzyme Production and Characterization
- Enzyme Catalysis and Immobilization
- Genomics and Phylogenetic Studies
- Enzyme Structure and Function
- Bacteriophages and microbial interactions
- Microbial Community Ecology and Physiology
- Protein purification and stability
- Polar Research and Ecology
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Reactions
- Microbial Metabolic Engineering and Bioproduction
- Synthesis and biological activity
- biodegradable polymer synthesis and properties
- Asymmetric Hydrogenation and Catalysis
- Salmonella and Campylobacter epidemiology
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Enzyme function and inhibition
- Viral gastroenteritis research and epidemiology
- Multicomponent Synthesis of Heterocycles
- Medicinal Plants and Neuroprotection
- Escherichia coli research studies
- Advancements in Transdermal Drug Delivery
- Heme Oxygenase-1 and Carbon Monoxide
- Methane Hydrates and Related Phenomena
Vellore Institute of Technology University
2024
King Abdullah University of Science and Technology
2017-2024
Maharishi Markandeshwar University, Mullana
2021-2024
Lala Lajpat Rai University of Veterinary and Animal Sciences
2024
University of Delhi
2024
Galgotias University
2021
University System of Maryland
2012-2017
University of Maryland, Baltimore
2012-2017
Indian Institute of Technology Indore
2010-2012
Indian Institute of Technology Delhi
2010-2011
Abstract Enzymes from extremophilic microorganisms usually catalyze chemical reactions in non-standard conditions. Such conditions promote aggregation, precipitation, and denaturation, reducing the activity of most non-extremophilic enzymes, frequently due to absence sufficient hydration. Some enzymes maintain a tight hydration shell remain active solution even when liquid water is limiting, e.g. presence high ionic concentrations, or at cold temperature close freezing point. Extremophilic...
Halophiles are excellent sources of enzymes that not only salt stable but also can withstand and carry out reactions efficiently under extreme conditions. The aim the study was to isolate diversity among halophilic bacteria producing industrial value. Screening halophiles from various saline habitats India led isolation 108 industrially important hydrolases (amylases, lipases proteases). Characterization 21 potential isolates by morphological, biochemical 16S rRNA gene analysis found them...
This paper intended to explore and discover recent therapeutic agents in the area of medicinal chemistry for treatment various diseases. Heterocyclic compounds represent an important group biologically active compounds. In last few years, heterocyclic having quinazoline moiety have drawn immense attention owing their significant biological activities. A diverse range molecules are reported show a broad activities like antifungal, antiviral, antidiabetic, anticancer, anti-inflammatory,...
Abstract Background Halorubrum lacusprofundi is a cold-adapted halophilic archaeon isolated from Deep Lake, perennially cold and hypersaline lake in Antarctica. Its genome sequencing project was recently completed, providing access to many genes predicted encode polyextremophilic enzymes active both extremely high salinity temperatures. Results Analysis of the sequence H. showed gene cluster for carbohydrate utilization containing glycoside hydrolase family 42 β-galactosidase gene, named bga...
The halophilic Archaeon Halorubrum lacusprofundi, isolated from the perennially cold and hypersaline Deep Lake in Antarctica, was recently sequenced compared to 12 Haloarchaea temperate climates by comparative genomics. Amino acid substitutions for 604 H. lacusprofundi proteins belonging conserved haloarchaeal orthologous groups (cHOGs) were determined found occur at 7.85% of positions invariant mesophilic Haloarchaea. following observed most frequently: (a) glutamic with aspartic or...
The haloarchaeon Halorubrum lacusprofundi is among the few polyextremophilic organisms capable of surviving in one most extreme aquatic environments on Earth, Deep Lake Antarctica (-18 °C to +11.5 and 21-28%, w/v salt content). Hence, H. has been proposed as a model for biotechnology astrobiology investigate potential life beyond Earth. To understand mechanisms that allow proteins adapt both salinity cold, we structurally (including X-ray crystallography molecular dynamics simulations)...
Poly(3-hydroxybutyrate) (PHB), a biodegradable polymer, can be produced by different microorganisms. The PHB belongs to the family of polyhydroxyalkanoate (PHA) that mostly accumulates as granule in cytoplasm microorganisms store carbon and energy. In this study, we established an integrated one-pot electromicrobial setup which dioxide is reduced formate electrochemically, followed sequential microbial conversion into PHB, using two model strains, Methylobacterium extorquens AM1 Cupriavidus...
Abstract The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA Pyl are extensively used to add non-canonical amino acids (ncAAs) the genetic code of bacterial eukaryotic cells. However, new ncAAs often require a cumbersome de novo engineering process generate an appropriate PylRS/tRNA pair. We here report strategy predict PylRS variant with novel properties. designed polyspecific HpRS catalyzes aminoacylation 31 structurally diverse bearing clickable, fluorinated, fluorescent, for...
Significance Combining comparative genomics, mutagenesis, kinetic analysis, and molecular modeling provides a powerful way to explore understand the structure function of proteins under extreme potentially astrobiological conditions. Alignment closely related cold-active mesophilic β-galactosidase enzymes from halophilic Archaea, followed by mutagenesis demonstrates importance specific amino acid residues in temperature-dependent catalytic activity, while structural framework for their...
A moderately haloalkaliphilic bacterium, Geomicrobium sp. EMB2, was isolated from the Sambhar Salt Lake located in western part of India. It secreted an alkaline protease, which stable and active presence a wide range organic solvents. The protease purified by hydrophobic interaction chromatography on Phenyl Sepharose 6 Fast Flow matrix, 22.6‐fold purification with 51.2% recovery attained. apparent molecular mass estimated to be 38 kDa. enzyme pH 6.0–12.0, optimum being 10.0. Km Vmax towards...
Gas vesicles are hollow, buoyant organelles bounded by a thin and extremely stable protein membrane. They coded cluster of gvp genes in the halophilic archaeon, Halobacterium sp. NRC-1. Using an expression vector containing entire gene cluster, gas vesicle nanoparticles (GVNPs) have been successfully bioengineered for antigen display constructing fusions between gvpC coding sequences from bacterial viral pathogens. To improve streamline genetic system bioengineering GVNPs, we first...
Environments previously thought to be uninhabitable offer a tremendous wealth of unexplored microorganisms and enzymes. In this paper, we present the discovery characterization novel γ-carbonic anhydrase (γ-CA) from polyextreme Red Sea brine pool Discovery Deep (2141 m depth, 44.8°C, 26.2% salt) by single-cell genome sequencing. The extensive analysis selected gene helps demonstrate potential culture-independent method. enzyme was expressed in bioengineered haloarchaeon Halobacterium sp....
Abstract Background Various bacteria and archaea, including halophilic archaeon Halobacterium sp. NRC-1 produce gas vesicle nanoparticles (GVNPs), a unique class of stable, air-filled intracellular proteinaceous nanostructures. GVNPs are an attractive tool for biotechnological applications due to their readily production, purification, physical properties. spindle- or cylinder-shaped, typically with length 100 nm 1.5 μm width 30–250 nm. Multiple monomeric subunits GvpA GvpC proteins form the...
Because only 0.01% of prokaryotic genospecies can be cultured and in situ observations are often impracticable, culture-independent methods required to understand microbial life harness potential applications microbes. Here, we report a methodology for the production proteins with desired functions based on single amplified genomes (SAGs) from unculturable species. We use this method resurrect an alcohol dehydrogenase (ADH/D1) uncharacterized halo-thermophilic archaeon collected brine pool...
A safe, effective, and inexpensive vaccine against typhoid other Salmonella diseases is urgently needed. In order to address this need, we are developing a novel platform employing buoyant, self-adjuvanting gas vesicle nanoparticles (GVNPs) from the halophilic archaeon Halobacterium sp. NRC-1, bioengineered display highly conserved enterica antigens. As initial antigen for testing, selected SopB, secreted inosine phosphate effector protein injected by pathogenic S. bacteria during infection...