A5028925724- Enzyme function and inhibition
- Enzyme Structure and Function
- Yersinia bacterium, plague, ectoparasites research
- Hemoglobin structure and function
- Microbial Fuel Cells and Bioremediation
- Plant Stress Responses and Tolerance
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Polyamine Metabolism and Applications
- Vector-borne infectious diseases
- Photosynthetic Processes and Mechanisms
- Magnetic and Electromagnetic Effects
- Advanced biosensing and bioanalysis techniques
- Biotin and Related Studies
- Mass Spectrometry Techniques and Applications
- Protein Structure and Dynamics
- Biofuel production and bioconversion
- RNA Interference and Gene Delivery
- Heat shock proteins research
- DNA and Nucleic Acid Chemistry
King Abdullah University of Science and Technology
2018-2020
Center for Integrated Protein Science Munich
2018-2020
Technical University of Munich
2010-2020
The haloarchaeon Halorubrum lacusprofundi is among the few polyextremophilic organisms capable of surviving in one most extreme aquatic environments on Earth, Deep Lake Antarctica (-18 °C to +11.5 and 21-28%, w/v salt content). Hence, H. has been proposed as a model for biotechnology astrobiology investigate potential life beyond Earth. To understand mechanisms that allow proteins adapt both salinity cold, we structurally (including X-ray crystallography molecular dynamics simulations)...
Enzymes originating from hostile environments offer exceptional stability under industrial conditions and are therefore highly in demand. Using single-cell genome data, we identified the alcohol dehydrogenase (ADH) gene, adh/a1a, Atlantis II Deep Red Sea brine pool. ADH/A1a is active at elevated temperatures high salt concentrations (optima 70 °C 4 m KCl) withstands organic solvents. The polyextremophilic exhibits a broad substrate scope including aliphatic aromatic alcohols able to reduce...
Environments previously thought to be uninhabitable offer a tremendous wealth of unexplored microorganisms and enzymes. In this paper, we present the discovery characterization novel γ-carbonic anhydrase (γ-CA) from polyextreme Red Sea brine pool Discovery Deep (2141 m depth, 44.8°C, 26.2% salt) by single-cell genome sequencing. The extensive analysis selected gene helps demonstrate potential culture-independent method. enzyme was expressed in bioengineered haloarchaeon Halobacterium sp....
In plants, much like in animals, nitric oxide (NO) has been established as an important gaseous signaling molecule. However, contrary to animal systems, NO-sensitive or NO-responsive proteins that bind NO the form of a sensor participating redox reactions have remained elusive. Here, we applied search term constructed based on conserved and functionally annotated amino acids at centers Heme Nitric Oxide/Oxygen (H-NOX) domains experimentally-tested gas-binding from lower higher eukaryotes,...
Abstract The biotin–streptavidin interaction is among the strongest known in nature. Herein, site‐directed incorporation of biotin and 2‐iminobiotin composed noncanonical amino acids (ncAAs) into proteins reported. 2‐Iminobiotin lysine was employed for protein purification based on pH‐dependent dissociation constant to streptavidin. By using high‐affinity binding lysine, bacterial RecA could be specifically isolated its partners analyzed. Furthermore, biotinylation approach successfully...