A5073488824- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Chronic Lymphocytic Leukemia Research
- Cell death mechanisms and regulation
- Iron Metabolism and Disorders
- Child Nutrition and Water Access
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
- Glycosylation and Glycoproteins Research
- Aluminum toxicity and tolerance in plants and animals
- Drug Transport and Resistance Mechanisms
- Metal complexes synthesis and properties
- Lymphoma Diagnosis and Treatment
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Asthma and respiratory diseases
- Molecular Sensors and Ion Detection
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- Blood groups and transfusion
- Toxin Mechanisms and Immunotoxins
- Pharmacological Effects of Medicinal Plants
- Diabetes and associated disorders
- Urticaria and Related Conditions
- Sinusitis and nasal conditions
Queen Elizabeth Hospital
2011-2022
The University of Adelaide
2013-2022
Basil Hetzel Institute
2012-2022
Jichi Medical University
2019
Royal Adelaide Hospital
2014
Collegium Medicum in Bydgoszcz
2008
South Australia Pathology
2002
Australian National University
2002
Zinquin [(2-methyl-8-p-toluenesulphonamido-6-quinolyloxy)-acetic acid], a membrane-permeant fluorophore specific for Zn(II), was used with spectrofluorimetry and video image analysis to reveal quantify labile intracellular Zn. labelled human chronic-lymphocytic-leukaemia lymphocytes, rat splenocytes thymocytes weak diffuse fluorescence that quenched when Zn chelated NNN‘N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) greatly intensified by pretreatment of cells the ionophore pyrithione...
We used an intracellular zinc-specific fluorophore, Zinquin, in conjunction with fluorescence video image analysis, to reveal labile zinc pancreatic islet cells, which concentrate this metal for use synthesis, storage, and secretion of insulin. Zinquin vividly demonstrated the cell secretory granules, formed a brightly labeled crescent cytoplasm between one side nucleus plasma membrane. Lower but still appreciable amounts were detected remaining cytoplasm, there was little labeling nucleus....
There is clinical evidence linking asthma with the trace element, zinc (Zn). Using a mouse model of allergic inflammation, we have previously shown that labile Zn decreases in inflamed airway epithelium (Truong-Tran AQ, Ruffin RE, Foster PS, Koskinen AM, Coyle P, Philcox JC, Rofe Zalewski PD. Am J Respir Cell Mol Biol 27: 286-296, 2002). Moreover, mild nutritional deficiency worsens lung function. Recently, number proteins belonging to Solute Carrier Family 39 (ZIP) and 30 (ZnT) been...
Zinquin [ethyl (2-methyl-8-p-toluenesulphonamido-6-quinolyloxy)acetate], a new intracellular zinc fluorophore, was used to reveal and measure Zn in cultured rat hepatocytes before after metallothionein (MT) induction. Hepatocytes labelled with an intense extranuclear fluorescence. Culture combinations of Zn, dexamethasone interleukin-6, increased MT by 24-fold, 3-fold, fluorescence approx. 2-fold above control values. correlated descending order the total cellular (r = 0.747), exchangeable...
Our previous studies have shown that nutritional zinc restriction exacerbates airway inflammation accompanied by an increase in caspase-3 activation and accumulation of apoptotic epithelial cells the bronchioles mice. Normally, are rapidly cleared macrophage efferocytosis, limiting any secondary necrosis inflammation. We therefore hypothesized deficiency is not only pro-apoptotic but also impairs efferocytosis. Impaired efferocytic clearance alveolar macrophages occurs chronic obstructive...
Protein kinase C was measured in the cytoskeletal fraction of lymphocytes, platelets and HL60 cells, by specific binding [ 3 H]phorbol dibutyrate immunoblotting with antibody to a consensus sequence regulatory domain α‐, β‐ γ‐isozymes protein C. Treatment cells for 40 min combination zinc (2–50 μM), ionophore pyrithione unlabelled phorbol (200 nM) caused up ten‐fold increase corresponding decrease other cellular compartments. Omission any reagents resulted much less or no translocation....
Scope An increased intake of Zinc (Zn) may reduce the risk degenerative diseases but prove to be toxic if taken in excess. This study aimed investigate whether zinc carnosine supplement can improve Zn status, genome stability events, and transporter gene expression an elderly (65–85 years) South Australian cohort with low plasma levels. Methods results A 12‐week placebo‐controlled intervention trial was performed 84 volunteers completing study, (placebo, n = 42) (Zn group, 42). Plasma...
The respiratory epithelium is vulnerable to noxious substances, resulting in the shedding of cells and decreased protection. Zinc (Zn), an antioxidant cytoprotectant, can suppress apoptosis a variety cells. Here we used novel Zn-specific fluorophore Zinquin visualize quantify labile intracellular Zn epithelial fluorescence isolated ciliated tracheobronchial intact from sheep pigs revealed intense apical mitochondria-rich cytoplasm below cilia. was quenched by chelator N, N′,...
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