A5014364470- Food Allergy and Anaphylaxis Research
- Allergic Rhinitis and Sensitization
- Insect and Pesticide Research
- Monoclonal and Polyclonal Antibodies Research
- Contact Dermatitis and Allergies
- Healthcare and Venom Research
- Animal health and immunology
- Urticaria and Related Conditions
- Virus-based gene therapy research
- Toxin Mechanisms and Immunotoxins
- RNA regulation and disease
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Biomedical and Chemical Research
- Lymphoma Diagnosis and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- CAR-T cell therapy research
- Drug-Induced Adverse Reactions
- Chronic Lymphocytic Leukemia Research
Universität Hamburg
2006-2022
University Medical Center Hamburg-Eppendorf
2011-2022
University Cancer Center Hamburg
2011-2016
Euroimmun Medizinische Labordiagnostika (Germany)
2016
Institute of Biochemistry
2010-2011
Gene therapy critically relies on vectors that combine high transduction efficiency with a degree of target specificity and can be administered through safe intravenous route. The lack suitable vectors, especially for gene brain disorders, represents major obstacle. Therefore, we applied an in vivo screening system random ligand libraries displayed adeno-associated viral capsids to select brain-targeted the treatment neurovascular diseases. We identified capsid variant showing unprecedented...
Insect stings can cause life-threatening IgE-mediated anaphylactic reactions in venom-allergic patients. Although several compounds have already been described as venom allergens, prominent allergen candidates especially the higher m.w. range still remained elusive. Tandem mass spectrometry-based sequencing assigned a candidate gene to most putative high Api m 5 (allergen C) honeybee (Apis mellifera) and also allowed identification of its homologue Ves v 3 yellow jacket (Vespula vulgaris)...
Generalized systemic reactions to stinging hymenoptera venom constitute a potentially fatal condition in venom-allergic individuals. Hence, the identification and characterization of all allergens is imperative for improvement diagnosis design effective immunotherapeutic approaches. Our aim was immunochemical carbohydrate-rich protein Api m 10, an Apis mellifera component putative allergen, with focus on relevance glycosylation. Furthermore, presence 10 honeybee (HBV) licensed immunotherapy...
Background/Objectives Anaphylaxis due to hymenoptera stings is one of the most severe clinical outcomes IgE-mediated hypersensitivity reactions. Although allergic reactions are often considered as a general model for underlying principles disease, venom immunotherapy still hampered by systemic side effects and incomplete protection. The identification detailed characterization all allergens venoms might result in an improvement this field promote understanding allergological mechanism. Our...
Hymenoptera venoms are known to cause life-threatening IgE-mediated anaphylactic reactions in allergic individuals. Proper diagnosis of hymenoptera venom allergy using extracts is severely affected by molecular cross-reactivities. Although non-glycosylated marker allergens would facilitate the identification culprit venom, major allergen phospholipase A1 (Ves v 1) from yellow jacket (YJV) remained unavailable so far.Expression Ves 1 as wild type and enzymatically inactivated mutant 5 insect...
Background Skin testing can expose allergic subjects to potential systemic reactions, sensitization against unrelated proteins, and increased risk of future sting reactions. Therefore the continuous improvement in vitro diagnostic methods is desirable. Recombinant allergens have been shown improve sensitivity specific IgE (sIgE) detection whilst no data available regarding their application reliability basophil activation test (BAT). Here we aimed compare specificity recombinant Ves v 1, 2,...
Allergic reactions to hymenoptera stings are one of the major reasons for IgE-mediated anaphylaxis. However, proper diagnosis using venom extracts is severely affected by molecular cross-reactivity. In this study recombinant honeybee allergen phospholipase A2 (Api m 1) was produced first time in insect cells. Using baculovirus infection different cell lines versions providing a varying degree cross-reactive carbohydrate determinants as well non glycosylated variant could be obtained secreted...
Allergen-specific IgE and IgG antibodies play pivotal roles in the induction progression of allergic hypersensitivity reactions. Consequently, monoclonal human IgG4 with defined specificity for allergens should be useful allergy research diagnostic tests. We used combinatorial antibody libraries subsequent recombinant production to make assess IgE, IgG1, allergen-specific antibodies.We phage display select a synthetic single-chain fragment (scFv) library against 3 different allergens, from...
Immune responses to N-glycan structures from allergens and parasites are often associated with pronounced, high affinity IgE reactivities. Cross-reactive carbohydrate determinants (CCDs) constituted by modified core represent the most frequently recognized epitopes in allergic immune responses. Although recently accepted as potentially allergenic epitopes, biological clinical relevance well structural functional characteristics of CCD-specific antibodies remain elusive.In order gain insights...
Receptor-targeted therapies have become standard in the treatment of various lymphomas. In view its unparalleled specificity for malignant B-cell clone, receptor (BCR) on B cell lymphoma cells is a potential therapeutic target. We used two BCR epitope mimicking peptides binding to Burkitt's lines CA46 and SUP-B8. proved their functionality by demonstrating calcium flux BCR-mediated endocytosis upon peptide binding. Toxicity experiments vitro via cross-linking with tetramerized mimics lead...
Objective Sting challenge is the gold standard method to evaluate therapeutic efficiency of allergen specific immunotherapy (ASIT) in hymenoptera venom allergic patients. Unfortunately, this risky, expensive and time consuming. Therefore, development an vitro desirable. Recently basophil activation test (BAT) performed with natural has been shown be a promising method. We aimed improve benefit BAT technology by applying panel recombinant allergens Ves v 1, 2, 3 5.