A5010841814- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Fungal and yeast genetics research
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
- Plant Molecular Biology Research
- Nuclear Structure and Function
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Chemical Synthesis and Analysis
- Single-cell and spatial transcriptomics
- Folate and B Vitamins Research
- Bacterial Genetics and Biotechnology
- Plant Reproductive Biology
- Innovative Microfluidic and Catalytic Techniques Innovation
- ATP Synthase and ATPases Research
- RNA regulation and disease
- Yeasts and Rust Fungi Studies
- Sulfur Compounds in Biology
- Viral Infectious Diseases and Gene Expression in Insects
Institut de Biologie Intégrative de la Cellule
2015-2024
Université Paris-Saclay
2019-2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2014-2024
Centre National de la Recherche Scientifique
2001-2024
CEA Paris-Saclay
2013-2024
Centre de Génétique Moléculaire
2004-2020
Université Paris-Sud
2013-2019
Frédéric Joliot Institute for Life Sciences
2017
Institut de Biologie et Technologies
2007-2016
École Polytechnique
1996-2004
Gene transcription is highly regulated. Altered can lead to cancer or developmental diseases. Mediator, a multisubunit complex conserved among eukaryotes, generally required for RNA polymerase II (Pol II) transcription. An interaction between the two complexes known, but its molecular nature and physiological role are unclear. We identify direct physical Rpb3 Pol subunit of Saccharomyces cerevisiae essential Mediator subunit, Med17. Furthermore, we demonstrate functional element in...
In Escherichia coli, tyrosyl-tRNA synthetase is known to esterify tRNATyr with tyrosine. Resulting d-Tyr-tRNATyr can be hydrolyzed by ad-Tyr-tRNATyr deacylase. By monitoring E. coli growth in liquid medium, we systematically searched for other d-amino acids, the toxicity of which might exacerbated inactivation gene encodingd-Tyr-tRNATyr addition already documented case d-tyrosine, positive responses were obtained d-tryptophan, d-aspartate,d-serine, and d-glutamine. agreement this...
RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of interacted via its C terminus with Rpb5, a conserved shared by all three nuclear RNA polymerases (Pol). Furthermore, complex coimmunoprecipitated polymerases. Mutations in conferred thermosensitive phenotype and loss interaction Rpb5. Certain rpb5 mutations were lethal combination rsc4 mutation, supporting physiological significance interaction. Pol II transcription ca. 12% yeast genome was...
RNA polymerase (Pol) III synthesizes the tRNAs, 5S ribosomal and a small number of untranslated RNAs. In vitro, it also transcribes short interspersed nuclear elements (SINEs). We investigated distribution Pol its associated transcription factors on genome mouse embryonic stem cells using highly specific tandem ChIP-Seq method. Only subset annotated class genes was bound thus transcribed. A few hundred SINEs were with machinery. observed that present at 30 unannotated sites genome, only one...
The transcription initiation and elongation steps of protein-coding genes usually rely on unrelated protein complexes. However, the TFIIS factor is implicated in both processes. We found that, absence Med31 Mediator subunit, yeast cells required polymerase II (Pol II)-binding domain but not its RNA cleavage stimulatory activity that associated with function. also Pol II-interacting was needed for full recruitment to several promoters Med31. This work demonstrated addition thoroughly...
TFIIS is a transcription elongation factor that stimulates transcript cleavage activity of arrested RNA polymerase II (Pol II). Recent studies revealed has also role in Pol initiation. To improve our understanding function vivo, we performed genome-wide location analysis this factor. Under normal growth conditions, was detected on II-transcribed genes, and occupancy well correlated with II, indicating recruitment not restricted to NTP-depleted cells. Unexpectedly, almost all III-transcribed...
Mediator is a large coregulator complex conserved from yeast to humans and involved in many human diseases, including cancers. Together with general transcription factors, it stimulates preinitiation (PIC) formation activates RNA polymerase II (Pol II) transcription. In this study, we analyzed how acts PIC assembly using vivo, vitro, silico approaches. We revealed an essential function of the middle module exerted through its Med10 subunit, implicating key interaction between TFIIB. showed...
d-Cysteine, a powerful inhibitor of Escherichia coli growth, is decomposedin vitro into pyruvate, H2S, and NH3 by d-cysteine desulfhydrase. To assess the role this reaction in adaptation bacterium to growth ond-cysteine, gene desulfhydrase was cloned. It corresponds open reading frame yedO at 43.03 min on genetic map E. coli. The amino acid sequence deduced from homologous those several 1-aminocyclopropane-carboxylate deaminases. However, theE. does not use 1-aminocyclopropane-1-carboxylate...
The yihZ gene of Escherichia coli is shown to produce a deacylase activity capable recycling misaminoacylated d-Tyr-tRNATyr. reaction specific and, under optimal in vitroconditions, proceeds at rate 6 s−1 with aK m value for the substrate equal 1 μm. Cell growth sensitive interruption theyihZ if d-tyrosine added minimal culture medium. Toxicity exogenous exacerbated if, addition disruption yihZ, d-amino acid dehydrogenase (dadA) also inactivated. Orthologs occur many, but not all, bacteria....
d-Tyr-tRNATyr deacylase cleaves the ester bond between a tRNA molecule and d-amino acid. In Escherichia coli, inactivation of gene (dtd) encoding this increases toxicity several acids including d-tyrosine, d-tryptophan, d-aspartic Here, we demonstrate that, in Δdtd cell grown presence 2.4 mm ∼40% total tRNATyr pool is converted into d-Tyr-tRNATyr. No observed dtd+ cells. addition, observe that overproduction tRNATyr, tRNATrp, or tRNAAsp protects mutant strain against toxic effect acid,...
Mediator is a large multiprotein complex conserved in all eukaryotes. The crucial function of transcription now largely established. However, we found that this also plays an important role by connecting with DNA repair. We identified functional contact between the Med17 subunit and Rad2/XPG, 3′ endonuclease involved nucleotide excision Genome-wide location analyses revealed Rad2 associated RNA polymerase II (Pol II)- Pol III-transcribed genes telomeric regions absence exogenous genotoxic...
Mediator is a large multiprotein complex conserved in all eukaryotes, which has crucial coregulator function transcription by RNA polymerase II (Pol II). However, the molecular mechanisms of its action vivo remain to be understood. Med17 an essential and central component head module. In this work, we utilised our collection conditional temperature-sensitive med17 mutants investigate Mediator's role coordinating preinitiation (PIC) formation at genome level after transfer non-permissive...
Chromatin organization is crucial for transcriptional regulation in eukaryotes. Mediator an essential and conserved co-activator thought to act concert with chromatin regulators. However, it remains largely unknown how their functions are coordinated. Here, we provide evidence the yeast Saccharomyces cerevisiae that establishes physical contact RSC (Remodels Structure of Chromatin), a remodeling complex nucleosome-depleted region (NDR) formation. We determine role Mediator-RSC interaction...
The Saccharomyces cerevisiae YDL219w (DTD1) gene, which codes for an amino acid sequence sharing 34% identity with the Escherichia coli d-Tyr-tRNATyr deacylase, was cloned, and its product functionally characterized. Overexpression in yeast of DTD1 gene from a multicopy plasmid increasedd-Tyr-tRNATyr deacylase activity crude extracts by two orders magnitude. Upon disruption chromosomal decreased more than 90%, sensitivity to d-tyrosine growth S. exacerbated. toxicity ofd-tyrosine also...
According to their role in translation, tRNAs specifically interact either with elongation factor Tu (EFTu) or initiation 2 (IF2). We here describe the effects of overproducing EFTu and IF2 on elongator versus initiator activities various mutant tRNAMet species vivo. The data obtained indicate that selection a tRNA through one other pathway translation depends relative amounts translational factors. A moderate overexpression is enough lead misappropriation process, whereas overproduced...
RNA polymerase (pol) III, assisted by the transcription factors TFIIIC and TFIIIB, transcribes small untranslated RNAs, such as tRNAs. In addition to known pol III-transcribed genes, Saccharomyces cerevisiae genome contains loci (ZOD1, ETC1-8) associated incomplete III complexes (Moqtaderi, Z., Struhl, K. (2004) Mol. Cell. Biol. 24, 4118-4127). We show that a short segment of ZOD1 locus, containing box A B promoter elements termination signal between them, directs III-dependent production...