A5034611665- Proteoglycans and glycosaminoglycans research
- Clinical Laboratory Practices and Quality Control
- Renal function and acid-base balance
- Protease and Inhibitor Mechanisms
- Neonatal Health and Biochemistry
- Cell Adhesion Molecules Research
- Electrolyte and hormonal disorders
- Methemoglobinemia and Tumor Lysis Syndrome
- Glycosylation and Glycoproteins Research
- Forensic Toxicology and Drug Analysis
- Connective tissue disorders research
- Heme Oxygenase-1 and Carbon Monoxide
- Acute Myocardial Infarction Research
- Cancer, Lipids, and Metabolism
- Blood transfusion and management
- Meta-analysis and systematic reviews
- Reliability and Agreement in Measurement
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Coronary Artery Anomalies
- Anesthesia and Sedative Agents
- Phenothiazines and Benzothiazines Synthesis and Activities
- Biosimilars and Bioanalytical Methods
- Pharmacological Receptor Mechanisms and Effects
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
Cleveland Clinic Lerner College of Medicine
2017-2024
Sanford Health
2023-2024
University of South Dakota
2023-2024
Yale University
1995-2022
Cleveland State University
2017-2020
Yale New Haven Health System
2020
Mayo Clinic
2009-2016
Mayo Clinic in Arizona
2012-2015
Proteoglycan accumulation is a hallmark of medial degeneration in thoracic aortic aneurysm and dissection (TAAD). Here, we defined the proteoglycanome using mass spectrometry, based on findings, investigated large aggregating proteoglycans aggrecan versican human ascending TAAD mouse model severe Marfan syndrome. The comprises 20 including versican. Antibodies against these intensely stained lesions TAAD, contrasting with modest intralamellar staining controls. Aggrecan, but not versican,...
Anticoagulation protocols used during mechanical circulatory support call for titration of antiplatelet agents. We compared the precision and reliability 5 platelet function tests in healthy volunteers donors on daily therapy to distinguish their efficacy titrating therapy.We assessed arachidonic acid-induced by light transmission aggregometry (LTA), Multiplate impedance aggregometry, VerifyNow, mapping thromboelastography (TEG PM). ADP-induced same methods flow cytometry. Forty 10-13...
Abstract ADAMTS (A Disintegrin-like and Metalloproteinase domain with Thrombospondin type 1 Motif)-1, -4 -5 share the abilities to cleave large aggregating proteoglycans including versican aggrecan. These activities are highly relevant cardiovascular disease osteoarthritis during development. Here, using purified recombinant ADAMTS-1, -5, we quantify, compare, define molecular basis of their versicanase activity. A novel sandwich-ELISA detecting major cleavage fragment was used determine,...
Two inherent challenges in the mechanistic interpretation of protease-deficient phenotypes are defining specific substrate cleavages whose reduction generates and determining whether result from loss function, accumulation, or a function(s) embodied fragments. Hence, recapitulation phenotype by cleavage-resistant would stringently validate importance proteolytic event clarify underlying mechanisms. Versican is large proteoglycan required for development circulatory system proper limb...
To compare thromboelastography (TEG) tracings obtained from fresh and citrated whole-blood samples in patients on extracorporeal membrane oxygenation (ECMO) or after cardiopulmonary bypass healthy volunteers.Samples of whole blood were analyzed for 25 4 volunteers. Thromboelastography analysis was performed both plain heparinase cups.In 5 6 ECMO, use resulted apparent partial complete heparin reversal. In TEG following bypass, there a slight hypercoagulable appearance the sample. No...
Leiomyomas have abundant extracellular matrix (ECM), with upregulation of versican, a large proteoglycan. We investigated ADAMTS (a disintegrin-like and metalloprotease thrombospondin type 1 motifs) protease-mediated versican cleavage in myometrium leiomyoma the effect knockdown cells. used quantitative reverse transcription polymerase chain reaction (qRT-PCR), western blotting, immunohistochemistry, RNA situ hybridization for analysis myometrium, immortalized Short interfering (siRNA) was...
Osteoarthritis (OA) is a highly prevalent joint disease, affecting millions of people worldwide and characterized by degradation articular cartilage, subchondral bone remodeling low-grade inflammation, leading to pain, stiffness disability. Cartilage Oligomeric Matrix Protein (COMP) major structural component cartilage its has been proposed as marker OA severity/progression. Several proteases cleave COMP in vitro, however, it unclear which these COMPase activities an osteoarthritic joint....
Previous studies have found positive bias and frequent outliers when central venous catheter (CVC) whole blood is used to dose glucose meters. We designed a study determine whether with CVC samples are due exogenous contamination of samples, inherent imprecision meters, or properties that interfere the function some meters.We studied relationship between plasma drawn by venipuncture in 50 hospitalized patients. In 27 patients was measured on both Accu-Chek Inform (Roche Diagnostics,...
Aims Pneumatic tube systems (PTSs) are critical for modern hospital operations, allowing rapid sample transport. Despite widespread use, PTSs can compromise specimen integrity and affect laboratory values. Our objective was to prove that serum clot tubes (RST) provide protective benefits over plasma during PTS transport be a practical solution certain routes. Methods In this study, we compared the effects of on cell lysis indicators: h-index, lactate dehydrogenase (LDH) potassium (K+), in...