A5017491621- Bee Products Chemical Analysis
- Insect and Pesticide Research
- Phytochemicals and Antioxidant Activities
- Healthcare and Venom Research
- Phenothiazines and Benzothiazines Synthesis and Activities
- Essential Oils and Antimicrobial Activity
- Drug Transport and Resistance Mechanisms
- Antioxidant Activity and Oxidative Stress
- Quinazolinone synthesis and applications
- Drug-Induced Hepatotoxicity and Protection
- Phytochemical and Pharmacological Studies
- Vitamin C and Antioxidants Research
- Engine and Fuel Emissions
- Plant and animal studies
- Free Radicals and Antioxidants
- Autophagy in Disease and Therapy
- Reproductive Biology and Fertility
- Berberine and alkaloids research
- Cancer Research and Treatment
- Pharmacological Effects and Toxicity Studies
- Technical Engine Diagnostics and Monitoring
- Cannabis and Cannabinoid Research
- Proteins in Food Systems
- Electron Spin Resonance Studies
- Agriculture and Biological Studies
Medical University of Silesia
2014-2024
University of Silesia in Katowice
2012-2024
Institute of Occupational Medicine and Environmental Health
2006
New York Academy of Medicine
1998
Breast cancer is one of the most common causes mortality in women. Flavonoids, among other compounds, are bioactive constituents propolis. In this comparative study, we investigated effects flavonoids apigenin (API), genistein (GEN), hesperidin (HES), naringin (NAR) and quercetin (QUE) on proliferation, apoptosis, cell cycle two different human cells - MDA-MB-231, estrogen-negative, MCF-7, estrogen-positive receptor breast carcinoma cells. Many cytotoxic reports were performed by MTT assay....
Propolis composition depends on several factors. The classification of propolis is based its geographical location, color and agricultural characteristics. It also classified according to the flora where bees collect resins, which represent raw material for production. possesses high antioxidant activity determined by phenolic compounds. Due diverse possible impact human health, eight samples were evaluated their activity. Samples Polish, Romanian, Turkish Uruguayan origin used spectrum...
One of the deadliest cancers among women is a breast cancer. Research has shown that two natural substances occurring in propolis, caffeic acid (CA) and phenethyl ester (CAPE), have significant anticancer effects. The purpose our vitro study was to compare cytotoxic activity migration rate inhibition using CA CAPE (doses 50 100 µm) against triple-negative, MDA-MB-231 adenocarcinoma line cells, drawn from Caucasian women. Viability measured by XTT-NR-SRB assay (Tetrazolium hydroxide-Neutral...
Studies show that caffeic acid (CA) and phenethyl ester (CAPE) are compounds with potent chemopreventive effects. Breast cancer is a common form of aggressive among women worldwide. This study shows comparison CA CAPE activity on triple-negative human caucasian breast adenocarcinoma line cells (MDA-MB-231). MDA-MB-231 were treated by doses from 10 to 100 µM, for periods 24 h 48 h. Cytotoxicity MTT tests, apoptosis Annexin V, cell cycle Dead Cell Assays performed. Cytotoxic was greater...
Chemotherapy of breast cancer could be improved by bioactive natural substances, which may potentially sensitize the carcinoma cells’ susceptibility to drugs. Numerous phytochemicals, including propolis, have been reported interfere with viability cells. We evaluated in vitro cytotoxic activity ethanol extract propolis (EEP) and its derivative caffeic acid phenethyl ester (CAPE) towards two triple-negative (TNBC) cell lines, MDA-MB-231 Hs578T, implementation MTT lactate dehydrogenase (LDH)...
Epithelium mammary carcinoma is a cancer with high death rate among women. One factor having significant impact on metastasis cell migration. The aim of this study was to compare migration inhibition caffeic acid (CA) and its phenethyl ester (CAPE) MCF-7 breast cells. Microscopic evaluation used determine the morphology cells, before after 24-hour treatment CA CAPE using dose 50 µM. cytotoxic effect measured by XTT-NR-SRB assay (tetrazolium hydroxide-neutral red-Sulforhodamine B) for 48-hour...
Medicines of biogenic origin with micro-organic, regenerative and analgesic properties are becoming more significant in the treatment burn wounds. These found apitherapeutics such as propolis honey—products collected processed by a honey bee. Their effect on course healing processes is multidirectional. The aim study was histopathological biochemical analysis scar formation experimentally evoked wounds white pigs treated 1% 3% Sepropol balms containing standardized extracts honey. results...
Reactive oxygen species participate in many physiological processes of the body, but their excess disturbs metabolic balance and leads to an unfavorable phenomenon called oxidative stress. Several mechanisms account for ROS elimination. One antioxidants may be phenothiazine derivatives because basic chemical structure – ring. The study aimed assess antioxidant activity prochlorperazine dimaleate, a derivative, compare it with reference (Trolox ascorbic acid). Thus, we investigated ABTS,...
The aim of this study was to determine the effect polyphenol-rich ethanol extract bee pollen (EEP) on atherosclerosis induced by a high-fat diet in ApoE-knockout mice. EEP given with feed two doses 0.1 and 1 g/kg body mass (BM). studies have been conducted period 16 weeks. following factors were estimated: total cholesterol (TC), oxidized low density lipoproteins (ox-LDL), asymmetric dimethylarginine (ADMA), angiotensin-converting enzyme (ACE) angiotensin II (ANG II) 5th, 10th, 12th, 14th,...
Antioxidative Properties of Bee Pollen Extracts Examined by EPR Spectroscopy pollen is a valuable and highly recognized source exogenous antioxidants. The aim these studies was to determine the antioxidant capacity three types bee extracts: ethanol extracts pollen, pepsin pepsin-digested pollen. Their properties were determined with use electron paramagnetic resonance (EPR) their ability quench DPPH free radicals estimated. results showed that (EEPP) had highest antioxidative effect radical...
We have studied a preventive effect of polyphenol-rich bee pollen ethanol extract (EEP) against histological changes in the liver and cardiac blood vessels, abnormalities lipid profile, levels oxidized low density lipoproteins (ox-LDL), asymmetric dimethylarginine (ADMA), angiotensin-converting enzyme (ACE), angiotensin II (ANG II) caused by high-fat diet C57BL₆ mice. Supplementing with EEP doses 0.1 g/kg body mass (BM) 1 BM resulted decrease total cholesterol 31% 35%, respectively. It also...
1) Background: Studies indicate that caffeic acid (CA), phenethyl ester (CAPE) are compounds with potent chemopreventive effects. Breast cancer is a common among women worldwide. The study shows comparison of and its activity in the cells breast line MDA-MB-231; 2) Methods: MDA-MB-231 were treated by CA CAPE doses from 10 to 100 µM time 24 h 48 h. Cytotoxicity MTT test, apoptosis Annexin V cell cycle Dead Cell Assay performed; 3) Results: cytotoxic was greater for comparing CA, both...
Our study determined the effect of extraction method bee pollen on optimal antioxidant activity extract obtained. We content polyphenols and flavonoids as well ethanol loads (EEP), pepsin (PEP), pepsin-digested (EEPP). The total was measured using Follin–Ciocalteau reagent. flavonoid aluminum chloride. Phenolic acids were identified quantified by HPLC. 2,2-diphenyl-picrylhydrazyl radical scavenging assays Trolox equivalents capacity. Antioxidant activities highest in EEPP associated with...
There is a great variation in the chemical composition of propolis different origins. Likewise, method its extraction has significant impact on content biologically active compounds. Here we compared methods for optimal antioxidant activities which were measured by means beta-carotene discolouration, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS*+) cation decolouration assays. In extracts, contents polyphenols...