Several studies have documented the ability of flavonoids to sensitize cancer cells chemotherapeutics and reverse multidrug resistance by inhibition efflux pumps (adenosine triphosphate-binding cassette transporters), apoptosis activation, cell cycle arrest. In this study, flavonoid rutin (quercetin 3-O-β-d-rutinoside) was investigated as chemosensitizer towards two different human epithelial breast lines: (i) MB-MDA-231, selected representative for triple-negative (ii) MCF-7 used a...

Synergistic interactions between commonly used antibiotics and natural bioactive compounds may exhibit therapeutic benefits in a clinical setting. Berberine, an isoquinoline-type alkaloid isolated from many kinds of medicinal plants, has proven efficacy against broad spectrum microorganisms. The aim the presented work was to assess antibacterial activity berberine chloride light effect exerted by common on fourteen reference strains Staphylococccus spp., evaluate magnitude with these...

Breast cancer is one of the most common causes mortality in women. Flavonoids, among other compounds, are bioactive constituents propolis. In this comparative study, we investigated effects flavonoids apigenin (API), genistein (GEN), hesperidin (HES), naringin (NAR) and quercetin (QUE) on proliferation, apoptosis, cell cycle two different human cells - MDA-MB-231, estrogen-negative, MCF-7, estrogen-positive receptor breast carcinoma cells. Many cytotoxic reports were performed by MTT assay....

Diet plays a crucial role in homeostasis maintenance. Plants and spices containing flavonoids have been widely used traditional medicine for thousands of years. Flavonols present our diet may prevent cancer initiation, promotion progression by modulating important enzymes receptors signal transduction pathways related to proliferation, differentiation, apoptosis, inflammation, angiogenesis, metastasis reversal multidrug resistance. The anticancer activity fisetin has documented numerous...

The objective of this study was to assess in vitro the antimicrobial activity ethanolic extract Polish propolis (EEPP) against methicillin-sensitive Staphylococcus aureus (MSSA) and methicillin-resistant (MRSA) clinical isolates. combined effect EEPP 10 selected antistaphylococcal drugs on S. cultures also investigated. composition analyzed by a High Performance Liquid Chromatography (HPLC) method. flavonoid compounds identified Propolis included flavones, flavonones, flavonolols, flavonols...

Dental caries occurrence is caused by the colonization of oral microorganisms and accumulation extracellular polysaccharides synthesized Streptococcus mutans with synergistic influence Lactobacillus spp. bacteria. The aim this study was to determine ex vivo antibacterial properties ethanol extract propolis (EEP), collected in Poland, against main cariogenic bacteria: salivary streptococci lactobacilli. isolation group bacteria (MS) (LB) from stimulated saliva performed in-office CRT dip...

One of the deadliest cancers among women is a breast cancer. Research has shown that two natural substances occurring in propolis, caffeic acid (CA) and phenethyl ester (CAPE), have significant anticancer effects. The purpose our vitro study was to compare cytotoxic activity migration rate inhibition using CA CAPE (doses 50 100 µm) against triple-negative, MDA-MB-231 adenocarcinoma line cells, drawn from Caucasian women. Viability measured by XTT-NR-SRB assay (Tetrazolium hydroxide-Neutral...

Studies show that caffeic acid (CA) and phenethyl ester (CAPE) are compounds with potent chemopreventive effects. Breast cancer is a common form of aggressive among women worldwide. This study shows comparison CA CAPE activity on triple-negative human caucasian breast adenocarcinoma line cells (MDA-MB-231). MDA-MB-231 were treated by doses from 10 to 100 µM, for periods 24 h 48 h. Cytotoxicity MTT tests, apoptosis Annexin V, cell cycle Dead Cell Assays performed. Cytotoxic was greater...

Chemotherapy of breast cancer could be improved by bioactive natural substances, which may potentially sensitize the carcinoma cells’ susceptibility to drugs. Numerous phytochemicals, including propolis, have been reported interfere with viability cells. We evaluated in vitro cytotoxic activity ethanol extract propolis (EEP) and its derivative caffeic acid phenethyl ester (CAPE) towards two triple-negative (TNBC) cell lines, MDA-MB-231 Hs578T, implementation MTT lactate dehydrogenase (LDH)...

Epithelium mammary carcinoma is a cancer with high death rate among women. One factor having significant impact on metastasis cell migration. The aim of this study was to compare migration inhibition caffeic acid (CA) and its phenethyl ester (CAPE) MCF-7 breast cells. Microscopic evaluation used determine the morphology cells, before after 24-hour treatment CA CAPE using dose 50 µM. cytotoxic effect measured by XTT-NR-SRB assay (tetrazolium hydroxide-neutral red-Sulforhodamine B) for 48-hour...

The aim of the presented study was to examine antimicrobial activity ethanol extract Polish propolis (EEPP) against biofilm-forming CoNS strains in vitro . Our results revealed that EEPP displayed varying degrees with MIC values ranging from 1.56 0.78 mg/mL. average 1.13 ± 0.39 mg/mL while calculated 50 and 90 were mg/mL, respectively. biofilm formation ability by all tested S. epidermidis inhibited at concentrations degree reduction AlamarBlue directly associated proliferation strains....

Natural polyphenols have been observed to possess antiproliferative properties. The effects, including apoptotic potential of bioactive phenolic compounds, caffeic acid (CA) and its derivative phenethyl ester (CAPE), on cell proliferation apoptosis in human head neck squamous carcinoma cells (HNSCC) line (Detroit 562) were investigated compared. Cancer rates cycle arrests analysed by flow cytometry. Exposure CA CAPE was found result a dose‐dependent decrease the viability Detroit 562 at...

Despite the relative effectiveness of standard cancer treatment strategies, head and neck (HNC) is still considered one leading causes mortality morbidity. While selected bioactive compounds plant origin reveal a pro-apoptotic effect, kaempferol fisetin flavonols have been reported as potential anti-cancer agents against malignant neoplasms. To date, their exact role in signaling pathways cells largely unknown. Based on various methods cytotoxicity testing, we elucidated that inhibit...

Reactive oxygen species participate in many physiological processes of the body, but their excess disturbs metabolic balance and leads to an unfavorable phenomenon called oxidative stress. Several mechanisms account for ROS elimination. One antioxidants may be phenothiazine derivatives because basic chemical structure – ring. The study aimed assess antioxidant activity prochlorperazine dimaleate, a derivative, compare it with reference (Trolox ascorbic acid). Thus, we investigated ABTS,...

Propolis is a natural product widely consumed in folk medicine. Different biological activities, such as anticancer, antioxidant, anti-inflammatory, antibiotic and antifungal effects have been reported for propolis its constituents.An vitro study focused on an evaluation of the activity EEPP, including anti-proliferative influence selected neoplastic cells, considering qualitative-quantitative chemical characterization Polish propolis.Cytotoxicity was evaluated by means MTT LDH assays. The...

Medicines of biogenic origin with micro-organic, regenerative and analgesic properties are becoming more significant in the treatment burn wounds. These found apitherapeutics such as propolis honey—products collected processed by a honey bee. Their effect on course healing processes is multidirectional. The aim study was histopathological biochemical analysis scar formation experimentally evoked wounds white pigs treated 1% 3% Sepropol balms containing standardized extracts honey. results...

Alcohol increases the risk of carcinoma originated from oral epithelium, but biological effects ultra-low doses ethanol on existing cells in combination with natural substances are still unclear. A role for (EtOH), taken small amounts as an ingredient some beverages or mouthwashes to change growth behavior established squamous cell carcinoma, has not been examined sufficiently. We designed vitro study determine effect caffeic acid (CFA) viability and migration ability malignant epithelial...

Ovarian cancer has the worst prognosis among all gynecological cancers. Therefore, it seems reasonable to seek new drugs that may improve effectiveness of treatment or mitigate adverse effects chemotherapy. Caffeic acid phenethyl ester (CAPE) many beneficial biological properties. The aim study was assess anticancer properties CAPE against serum ovarian carcinoma cells. morphology cells evaluated in H-E staining and transmission electron microscopy. cytotoxic proapoptotic activity...

Among many infectious diseases, infections caused by pathogens of Staphylococcus species exert a substantial influence upon human health, mainly due to their continuous presence on skin and mucous membranes. For that reason, an intensive search for new, effective anistaphyloccocal agents can currently be observed worldwide. In recent years, there has been growing interest in nanoparticles, as compounds with potential antibacterial effect. The activity silver containing substances well...

We have studied a preventive effect of polyphenol-rich bee pollen ethanol extract (EEP) against histological changes in the liver and cardiac blood vessels, abnormalities lipid profile, levels oxidized low density lipoproteins (ox-LDL), asymmetric dimethylarginine (ADMA), angiotensin-converting enzyme (ACE), angiotensin II (ANG II) caused by high-fat diet C57BL₆ mice. Supplementing with EEP doses 0.1 g/kg body mass (BM) 1 BM resulted decrease total cholesterol 31% 35%, respectively. It also...

1) Background: Studies indicate that caffeic acid (CA), phenethyl ester (CAPE) are compounds with potent chemopreventive effects. Breast cancer is a common among women worldwide. The study shows comparison of and its activity in the cells breast line MDA-MB-231; 2) Methods: MDA-MB-231 were treated by CA CAPE doses from 10 to 100 µM time 24 h 48 h. Cytotoxicity MTT test, apoptosis Annexin V cell cycle Dead Cell Assay performed; 3) Results: cytotoxic was greater for comparing CA, both...