A5085400068- Circadian rhythm and melatonin
- Neonatal Respiratory Health Research
- Spaceflight effects on biology
- Asthma and respiratory diseases
- Neuroscience of respiration and sleep
- RNA and protein synthesis mechanisms
- Dietary Effects on Health
- Immune Cell Function and Interaction
- Climate Change and Health Impacts
- Genomics and Phylogenetic Studies
- Influenza Virus Research Studies
- Respiratory viral infections research
- Birth, Development, and Health
- Congenital Diaphragmatic Hernia Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Gut microbiota and health
- Immune cells in cancer
- Genomics, phytochemicals, and oxidative stress
- COVID-19 Clinical Research Studies
- Respiratory Support and Mechanisms
- Diabetes and associated disorders
- Genetics, Aging, and Longevity in Model Organisms
- Molecular Biology Techniques and Applications
- Pediatric health and respiratory diseases
- Immune responses and vaccinations
Children's Hospital of Philadelphia
2014-2025
University of Pennsylvania
2012-2024
Philadelphia University
2020-2024
Translational Therapeutics (United States)
2018-2024
California University of Pennsylvania
2013
All India Institute of Medical Sciences
2003
Gurukul Kangri Vishwavidyalaya
1991
Full-term neonates born between 37 and 41 weeks' gestational age have been considered a homogeneous, low-risk group. However, recent evidence from studies based on mode of delivery has pointed toward increased morbidity associated with early-term cesarean section births (37-38 weeks) compared term (39-41 weeks).To compare the short-term vs in county-based birth cohort using primary objective admission to neonatal intensive care unit (NICU) or neonatology service.Retrospective...
Influenza is a leading cause of respiratory mortality and morbidity. While inflammation essential for fighting infection, balance anti-viral defense host tolerance necessary recovery. Circadian rhythms have been shown to modulate inflammation. However, the importance diurnal variability in timing influenza infection not well understood. Here we demonstrate that endogenous affect survival infection. control mediated by enhanced as proven increased cellularity bronchoalveolar lavage (BAL),...
The response to oxidative stress and inflammation varies with diurnal rhythms. Nevertheless, it is not known whether circadian genes are regulated by these stimuli. We evaluated Rev-erbα, a key gene, was and/or in vitro mouse model.A unique sequence consisting of overlapping AP-1 nuclear factor kappa B (NFκB) consensus sequences identified on the Rev-erbα promoter. This mediates promoter activity transcription inflammation. region serves as an NrF2 platform both receive signals activate well...
Neutrophil extracellular traps (NETs) provide host defense but can contribute to the pathobiology of diverse human diseases. We sought determine extent and mechanism by which NETs airway cell inflammation. Primary normal bronchial epithelial cells (HBEs) grown at air-liquid interface wild-type (wt)CFBE41o- (expressing wtCFTR) were exposed cell-free from unrelated healthy volunteers for 18 h in vitro. Cytokines measured apical supernatant Luminex, effect on HBE transcriptome was assessed RNA...
Many normal functions and pathologies trace back to RNA splicing. As such, splicing is currently the focus of a vast array studies. To quantify transcriptome, short read RNA-Seq remains standard assay. The primary technical artifact library prep, which severely interferes with analysis, extreme non-uniformity in coverage across transcripts. This present both bulk single-cell observed even when sample contains only full-length issue dramatically affects accuracy isoform-level quantification...
We have previously demonstrated that the circadian clock regulates host response to influenza A virus (IAV) infection, conferring a time-of-day-specific protection; infection at dawn resulted in threefold increase survival and reduced immunopathology compared dusk. While IL–10 is well-known for its immunoregulatory function, role IAV remains unclear, with studies reporting both protective detrimental effects. Given diurnal rhythmicity of receptor (Il10ra) expression lung, we investigated...
Influenza and other respiratory viral pathogens are leading causes of mortality morbidity. We previously demonstrated that circadian rhythms confer temporal protection from influenza infection. Here, we investigated whether this requires rhythmic function after the initial infection by manipulating environmental cycles. demonstrate disrupting lighting cues within a critical window vulnerability abrogates time-of-day specific protection. This poor outcome is mediated dysregulated immune...
Abstract Fibroblasts are stromal cells abundant throughout tissues, including the lungs. integral coordinators of immune cell recruitment through chemokine secretion. Circadian rhythms direct to lung, which in turn impacts response infection and survival. Although fibroblasts display robust circadian rhythms, contribution fibroblast molecular clock lung‐specific migration remains be established. Mice challenged intranasally with lipopolysaccharide (LPS) at dusk showed increased expression...
Premature infants exposed to hyperoxia suffer acute and long-term pulmonary consequences. Nevertheless, neonates survive better than adults. The factors contributing neonatal hyperoxic tolerance are not fully elucidated. In contrast adults, heme oxygenase (HO)-1, an endoplasmic reticulum (ER)-anchored protein, is abundant in the lung but inducible response hyperoxia. latter may be important, because very high levels of HO-1 overexpression associated with significant oxygen cytotoxicity...
Adverse early-life exposures have a lasting negative impact on health. Neonatal hyperoxia that is risk factor for bronchopulmonary dysplasia confers susceptibility to influenza A virus (IAV) infection later in life. Given our previous findings the circadian clock protects against IAV, we asked if long-term of neonatal vis-à-vis IAV includes disruption. Here, show abolishes clock-mediated time day protection from mice, independent viral burden through host tolerance pathways. We discovered...
<b>Objective</b> To compare the efficacy of vinyl bags and thermal warming mattresses in preventing hypothermia during delivery room resuscitation stabilization extremely low-gestational-age neonates (ELGANs). <b>Study Design</b> Preterm infants 23 to 28 weeks' gestational age were randomly allocated either bag or mattress group at prevent hypothermia. The primary outcome study was axillary temperature on admission neonatal intensive care unit. <b>Results</b> A total 41 enrolled study. mean...
Simulation of RNA-seq reads is critical in the assessment, comparison, benchmarking and development bioinformatics tools. Yet field simulators has progressed little last decade. To address this need we have developed BEERS2, which combines a flexible highly configurable design with detailed simulation entire library preparation sequencing pipeline. BEERS2 takes input transcripts (typically fully length messenger RNA polyA tails) from either customizable or CAMPAREE simulated samples. It...
Abstract The immunological defects causing susceptibility to severe viral respiratory infections due early-life dysbiosis remain ill-defined. Here, we show that influenza virus in dysbiotic infant mice is caused by CD8 + T cell hyporesponsiveness and diminished persistence as tissue-resident memory cells. We describe a previously unknown role for nuclear factor interleukin 3 (NFIL3) repression of differentiation cells involving epigenetic regulation 1 (TCF 1) expression. Pulmonary from human...
Optimal lung repair and regeneration are essential for recovery from viral infections, including influenza A virus (IAV). We have previously demonstrated that acute inflammation mortality induced by IAV is under circadian control. However, it not known whether the influence of clock persists beyond outcomes. Here, we utilize UK Biobank to demonstrate an association between poor rhythms morbidity lower respiratory tract need hospitalization after discharge; this even adjusting common...
Abstract It is common to measure large numbers of features identify those differing between experimental conditions; for example using RNA-Seq search differentially expressed genes. Ranking by p-value allows statistical control, but has well known issues: unreliability without many replicates; and significance biologically irrelevant effect sizes. As a result prioritization typically performed in conjunction with size; the canonical one being “fold-change” . However fold-change several...