A5006888400- Genetic Neurodegenerative Diseases
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Lysosomal Storage Disorders Research
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Sperm and Testicular Function
- Nerve injury and regeneration
- Autophagy in Disease and Therapy
- Bone Tissue Engineering Materials
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Orthopedic Infections and Treatments
- Pluripotent Stem Cells Research
- Neurological and metabolic disorders
- Cellular transport and secretion
- Pneumonia and Respiratory Infections
- Radio Wave Propagation Studies
- Physical Education and Sports Studies
- MXene and MAX Phase Materials
- Respiratory and Cough-Related Research
- Education Pedagogy and Practices
- Hematological disorders and diagnostics
- Neurogenetic and Muscular Disorders Research
- 3D Printing in Biomedical Research
University of Coimbra
2018-2024
Institute for Biomedicine
2020
San Antonio College
2013-2016
Universidade do Porto
2011-2014
Instituto de Biologia Molecular e Celular
2013
Institute of Biomedical Science
2012
Abstract Lung metastases represent the most adverse clinical factor and rank as leading cause of osteosarcoma‐related death. Nearly 80% patients present lung micrometastasis at diagnosis not detected with current tools. Herein, an exosome (EX)‐based imaging tool is developed for by positron emission tomography (PET) using osteosarcoma‐derived EXs natural nanocarriers positron‐emitter copper‐64 ( 64 Cu). Exosomes are isolated from metastatic osteosarcoma cells functionalized macrocyclic...
Machado-Joseph disease (MJD) or spinocerebellar ataxia type 3, the most common dominant (SCA) worldwide, is caused by over-repetition of a CAG repeat in ATXN3/MJD1 gene, which translates into polyglutamine tract within ataxin-3 protein. There no treatment for this fatal disorder. Despite evidence safety and efficacy mesenchymal stromal cells (MSCs) delaying SCA progression exploratory clinical trials, unanticipated regression patients to status prior makes investigation causes solutions...
Abstract Blood-brain barrier (BBB) disruption is a common feature in neurodegenerative diseases. However, BBB integrity has not been assessed spinocerebellar ataxias (SCAs) such as Machado-Joseph disease/SCA type 3 (MJD/SCA3), genetic disorder, triggered by polyglutamine-expanded ataxin-3. To investigate that, was evaluated transgenic mouse model of MJD and human post-mortem brain tissues. Firstly, we investigated the permeability mice by: i) assessing extravasation Evans blue (EB) dye...
In Krabbe's disease, a demyelinating disorder, add-on strategies targeting the peripheral nervous system (PNS) are needed, as it is not corrected by bone-marrow (BM) transplantation. To circumvent this limitation of BM transplantation, we assessed whether i.v. delivery immortalized EGFP(+) BM-derived murine mesenchymal stromal cells (BM-MSC(TERT-EGFP) ) targets PNS disease model, Twitcher mouse. vitro, BM-MSC(TERT-EGFP) retained phenotype primary BM-MSC and did originate tumors upon...
Efforts have been made for the isolation and characterization of human stem spermatogonia (SG) which would be major interest fertility preservation in oncologic patients. We evaluated expression mammalian SG cell markers, KIT, OCT4, integrin alpha 6 (ITGA6), beta 1 (ITGB1) as possible indicators those cells humans. Two different types were individually isolated by micromanipulation from testicular biopsies men with conserved spermatogenesis. Expression mRNA showed absence KIT ITGB1 markers...
This study investigates the osteogenic differentiation of umbilical-cord-derived human mesenchymal stromal cells (hUC-MSCs) on biphasic calcium phosphate (BCP) scaffolds derived from cuttlefish bone doped with metal ions and coated polymers. First, in vitro cytocompatibility undoped ion-doped (Sr2+, Mg2+ and/or Zn2+) BCP was evaluated for 72 h using Live/Dead staining viability assays. From these tests, most promising composition found to be scaffold strontium (Sr2+), magnesium (Mg2+) zinc...
Abstract Machado-Joseph disease (MJD) or Spinocerebellar ataxia type 3 (SCA3) is the most common form of dominant SCA worldwide. Magnetic Resonance Imaging (MRI) and Proton Spectroscopy ( 1 H-MRS) provide promising non-invasive diagnostic follow-up tools, also serving to evaluate therapies efficacy. However, pre-clinical studies showing relationship between MRI-MRS based biomarkers functional performance are missing, which hampers an efficient clinical translation therapeutics. This study...
The present study deals with the development of multifunctional biphasic calcium phosphate (BCP) scaffolds coated biopolymers-poly(ε-caprolactone) (PCL) or poly(ester urea) (PEU)-loaded an antibiotic drug, Rifampicin (RFP). amounts RFP incorporated into PCL and PEU-coated were 0.55 ± 0.04 0.45 0.02 wt%, respectively. in vitro drug release profiles buffered saline over 6 days characterized by a burst within first 8h, followed sustained release. Korsmeyer-Peppas model showed that was...
Krabbe's disease (KD) is a demyelinating disorder caused by the deficiency of lysosomal galactocerebrosidase (GALC), affecting both central (CNS) and peripheral nervous system (PNS). A current therapy, hematopoietic stem cell transplantation (HSCT), ineffective at correcting PNS pathology. We have previously shown that systemic delivery immortalized bone marrow-derived murine mesenchymal stromal cells (BM-MSCs) diminishes neuropathology transplanted Twitcher mice, model KD. In this study, to...
Recently, an innovative low-cost approach for the construction of meteorological radars has been introduced by exploiting correlation between received signals from two fixed wide-beam antennas. Yet, it was then found that a very large amount signal samples would be required to ensure satisfactory performance proposed radar. On other hand, also envisaged such problem could circumvented use more than This work is first step in this direction, extending original radar proposal arbitrary number...
Event Abstract Back to Autophagy activation induced by let-7 microRNA overexpression counteracts disease phenotype in Machado-Joseph mouse models Sónia P. Duarte1, 2, Beatriz Estremores1, Janete Cunha-Santos1, 3, Catarina O. Miranda1, João Barata1, Albert La Spada4 and Luis De Almeida1, 3* 1 Centre for Neuroscience Cell Biology, University of Coimbra, Portugal 2 Institute Interdisciplinary Research, 3 Faculty Pharmacy, 4 Department Neurology, School Medicine, Duke University, United States...
Aim: Acute otitis media (AOM) is a common illness in children and accounts for significant proportion of antibiotic prescriptions. However use controversial since the disease may be self-limiting recurrent childhood excessive lead to increased bacterial resistance. Xylitol has potential preventing AOM. This review examines evidence effectiveness xylitol AOM children. Data sources: National Guideline Clearinghouse, NHS Evidence, Canadian Medical Association, Scottish Intercollegiate...
Introduction: Extrapulmonary tuberculosis is found in 29% of cases Portugal. The most common sites are lymph nodes, pleura, bone, joints, and meninges. high prevalence Portugal the scarcity reported soft tissues highlight importance this case report. Case presentation: A 17 year-old female was healthy until February 2013 when she began to complain pain on movement right elbow. There no history trauma. She treated with anti-inflammatory medication. Because persistent appearance signs local...
Spermatogenesis is a complex process of cell proliferation, meiosis and differentiation. In order to determine the genetic mechanisms that control this we need develop adequate methodologies for purification stage-specific germ cells. study aim evaluate four markers (c-kit, oct-4, Integrin α 6 β 1 ), used isolate spermatogonial stem cells in mammals, as possible isolate/sort testicular adult progenitor humans.
Event Abstract Back to Blood-brain barrier dysfunction as a neuropathological feature in Machado-Joseph Disease: results from human and mouse samples Diana D. Lobo1, 2, Rui J. Nobre1, 3, 4, Catarina O. Miranda1, Dina Pereira1, João Castelhano5, José Sereno5, Miguel Castelo-Branco5, 6 Luís P. De Almeida1, 7* 1 Centro de Neurociências e Biologia Celular, Universidade Coimbra, Portugal 2 University of 3 Institute for Interdisciplinary Research, 4 Viral Vector Gene Transfer Core Facility, 5...
Event Abstract Back to Non-invasive and allele-specific silencing therapy for Polyglutamine disorders: the case of Machado-Joseph disease/Spinocerebellar ataxia type 3 Rui J. Nobre1, 2, 3*, Joana Saraiva1, Susana Paixão1, Clelia Fusco1, Magda Santana1, Catarina O. Miranda1, Lorena Petrella4, Jose Sereno4, Joao Castelhano4, Miguel Castelo-Branco4, Sena-Esteves5 Luis Pereira De ALmeida1, 3, 6 1 Centre Neuroscience Cell Biology, University Coimbra, Portugal 2 Institute Interdisciplinary...