A5043194603- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Bladder and Urothelial Cancer Treatments
- Glutathione Transferases and Polymorphisms
- Ubiquitin and proteasome pathways
- Tissue Engineering and Regenerative Medicine
- Renal and related cancers
- Chromosomal and Genetic Variations
- Cancer, Hypoxia, and Metabolism
- Genomics, phytochemicals, and oxidative stress
- Immunotherapy and Immune Responses
- Genomics and Chromatin Dynamics
- Redox biology and oxidative stress
- Immune cells in cancer
- Alcohol Consumption and Health Effects
- Sulfur Compounds in Biology
- Urological Disorders and Treatments
- RNA and protein synthesis mechanisms
- Eicosanoids and Hypertension Pharmacology
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Ferroptosis and cancer prognosis
Chulalongkorn University
2017-2023
Heinrich Heine University Düsseldorf
2020-2021
Promoter hypermethylation of the runt-related transcription factor 3 (RUNX3) gene is associated with increased risk hepatocellular carcinoma (HCC). Oxidative stress plays a vital role in both carcinogenesis and progression HCC. However, whether oxidative RUNX3 HCC have cause- and-effect relationship not known. In this study, plasma protein carbonyl total antioxidant capacity (TAC) patients hepatitis B virus (HBV)-associated (n=60) age-matched healthy subjects (n=80) was determined....
Background/Aim: Reactivation of long interspersed nuclear element-1 (LINE-1) and oxidative stress are suggested to have oncogenic potential drive tumorigenesis cancer progression. We previously demonstrated that reactive oxygen species (ROS) caused hypomethylation LINE-1 elements in bladder cells. In this study, we investigated the expression LINE-1-encoded protein (ORF1p) marker 4-hydroxynonenal (4-HNE) human tissues, as well induction ORF1p by ROS cell lines. Materials Methods: Thirty-six...
Human genomes contain about 100,000 LINE-1 (L1) retroelements, of which more than 100 are intact. L1s normally tightly controlled by epigenetic mechanisms, often fail in cancer. In bladder urothelial carcinoma (UC), particularly, become DNA-hypomethylated, expressed and contribute to genomic instability tumor growth. It is, however, unknown individual activated. Following RNA-immunoprecipitation with a L1-specific antibody, third generation nanopore sequencing detected transcripts 90...
Epigenetic alteration by oxidative stress is vitally involved in carcinogenesis and cancer progression. Previously, we demonstrated that was increased hepatocellular carcinoma (HCC) patients associated with tumor aggressiveness. Herein, immunohistochemically investigated whether histone methylation, specifically H4K20me3, upregulated human hepatic tissues obtained from HCC (n = 100). Also, experimentally explored if the H4K20me3 reactive oxygen species (ROS) contributed to progression cell...
The histone demethylase UTX (gene: KDM6A) directs cell and tissue differentiation during development. Deleterious mutations in KDM6A occur many human cancers, most frequently urothelial carcinoma. consequences of these are poorly understood; plausibly, they may disturb differentiation. We therefore investigated the effects siRNA-mediated knockdown two vitro models differentiation; namely, primary cultures epithelial cells treated with troglitazone PD153035 immortalized line HBLAK high...
Abstract Background KDM6A, encoding a histone demethylase, is one of the top ten mutated epigenetic cancer genes. The effect mutations on its structure and function are however poorly characterized. Methods Database search identified nonsense missense in N-terminal TPR motifs C-terminal, catalytic JmjC domain, but also intrinsically disordered region connecting both these two well-structured domains. KDM6A variants with cancer-derived were generated using site directed mutagenesis fused to...
Abstract Background KDM6A, encoding a histone demethylase, is one of the top ten mutated epigenetic cancer genes. The effect mutations on its structure and function are however poorly characterized. Methods Database search identified nonsense missense in N-terminal TPR motifs C-terminal, catalytic JmjC domain, but also intrinsically disordered region connecting both well-structured domains. KDM6A variants with cancer-derived were generated using site directed mutagenesis fused to eGFP, which...