Despite the success in clinical application, exact mechanism of shock wave therapy remains unknown. We hypothesized that induces ingrowth neovascularization and improves blood supply to tissues. The purpose this study was investigate effect on at tendon-bone junction. Fifty New Zealand white rabbits with body weight ranging from 2.5 3.5 kg were used study. right limb (the side) received Achilles tendon near insertion bone. left control no therapy. Biopsies junction performed 0, 1, 4, 8 12...

Abstract Extracorporeal shock waves (ESW) have recently been used in resolving tendinitis. However, mechanisms by which ESW promote tendon repair is not fully understood. In this study, we reported that an optimal treatment promoted healing of Achilles tendintis inducing TGF‐β1 and IGF‐I. Rats with the collagenease‐induced tendinitis were given a single (0.16 mJ/mm 2 energy flux density) 0, 200, 500 1000 impulses. tendons subjected to biomechanical (load failure stiffness), biochemical...

There is continuing controversy regarding the optimal treatment for patients with symptomatic early-stage osteonecrosis of femoral head. We compared results noninvasive extracorporeal shock waves those core decompression and bone-grafting in similar groups patients.Patients stage-I, II, or III were randomly assigned to be treated either nonvascularized fibular grafting. The shock-wave group consisted twenty-three (twenty-nine hips), surgical twenty-five (twenty-eight hips). two had...

Vascular endothelial growth factor (VEGF) released by osteoblasts plays an important role in angiogenesis and endochondral ossification during bone formation. In animal studies, we have reported that shock waves (SW) can promote osteogenic differentiation of mesenchymal stem cells through superoxide-mediated signal transduction (Wang, F. S., Wang, C. J., Sheen-Chen, S. M., Kuo, Y. R., Chen, R. F., Yang, K. D. (2002) J. Biol. Chem. 277, 10931–10937) vascularization the bone-tendon junction....

Extracorporeal shock wave (ESW) treatment has recently been established as a method to enhance bone repair. Here, we reported that ESW-promoted healing of segmental defect via stimulation mesenchymal stem cell recruitment and differentiation into forming cells. Rats with femoral were exposed single ESW (0.16 mJ/mm(2), 1 Hz, 500 impulses). Cell morphology histological changes in the region assessed 3, 7, 14, 28 days post-treatment. Presence was assayed by immuno-staining for RP59, discovered...

Glomerulosclerosis and diabetic nephropathy are attributable to high glucose induction of mesangial cell apoptosis. Whereas Wnt signaling has been found regulate renal morphogenesis pathogenesis, the biologic role Wnt/beta-catenin in controlling glucose-induced apoptosis is not well defined. Herein reported that required for protecting glomerular cells from glucose-mediated High downregulated Wnt4 Wnt5a expression subsequent nuclear translocation beta-catenin, whereas it increased glycogen...

Podocyte dysfunction is a detrimental feature in diabetic nephropathy, with loss of nephrin integrity contributing to podocytopathy. MicroRNAs (miRs) reportedly modulate the hyperglycemia-induced perturbation renal tissue homeostasis. This study investigated whether regulation histone deacetylase (HDAC) actions and acetylation by miR-29 contributes podocyte homeostasis function kidneys. Hyperglycemia accelerated injury reduced nephrin, acetylated miR-29a levels primary glomeruli from...

Long-term glucocorticoid treatment impairs the survival and bone formation of osteogenic cells, leading to mass loss. The Wnt inhibitor Dickkopf-1 (DKK1) acts as a potent bone-remodeling factor that mediates several types skeletal disorders. Whereas excess is known disturb signaling in modulation skeletally deleterious effects DKK1 alleviate induction loss has not been tested. In this study, knockdown expression by end-capped phosphorothioate antisense oligonucleotide (DKK1-AS) abrogated...

ABSTRACT Extracorporeal shock‐wave therapy (ESWT) has a significant positive effect in accelerating chronic wound healing. However, the bio‐mechanisms operating during ESWT of wounds remain unclear. This study investigated effectiveness enhancement diabetic A dorsal skin defect (area, 6 × 5 cm) streptozotocin‐induced diabetes rodent model was used. Fifty male Wistar rats were divided into five groups. Group I consisted nondiabetic control; group II included control receiving no ESWT; III...

OBJECTIVE Disturbances in podocytes are typically associated with marked proteinuria, a hallmark of diabetic nephropathy. This study was conducted to investigate modulation Notch-1 signaling high glucose (HG)-stressed human and animal model. RESEARCH DESIGN AND METHODS Expression the Notch components examined HG-treated podocytes, embryonic kidney cells (HEK293), kidneys from animals by RT-qPCR, Western blot analysis, immunohistochemical staining. The association between signaling, VEGF...

Compromised autophagy and mitochondrial dysfunction downregulate chondrocytic activity, accelerating the development of osteoarthritis (OA). Irisin, a cleaved form fibronectin type III domain containing 5 (FNDC5), regulates bone turnover muscle homeostasis. Little is known about effect Irisin on chondrocytes osteoarthritis. This study revealed that human osteoarthritic articular express decreased level FNDC5 autophagosome marker LC3-II but upregulated levels oxidative DNA damage...

Excessive glucocorticoid treatment increases the incidence of osteopenia and osteonecrosis. MicroRNAs (miRNAs) reportedly target messenger RNA expression regulate osteoblastogenesis skeletal development. We undertook this study to investigate whether miR-29a regulates glucocorticoid-mediated bone loss.Rats were given methylprednisolone, lentivirus-mediated precursor, or inhibitor. Dual x-ray absorptiometry, micro-computed tomography, material testing, enzyme-linked immunosorbent assay...

Abstract Chondrocyte loss is a prominent feature of osteoarthritis (OA). Autophagy indispensable in maintaining the metabolic activities cells exposed to deleterious stress. The contribution microRNA signaling chondrocyte autophagy OA development remains elusive. We uncovered an association between poor and increased miR-128a expressions articular chondrocytes patients with end-stage knee rat anterior cruciate ligament transection (ACLT) model for development. Cartilage matrix degradation...

MicroRNA-29 (miR-29) has been shown to play a critical role in reducing inflammation and fibrosis following liver injury. Non-alcoholic fatty disease (NAFLD) occurs when fat is deposited (steatosis) the due causes other than excessive alcohol use associated with fibrosis. In this study, we asked whether miR-29a could reduce experimental high diet (HFD)-induced obesity mice. We performed systematical expression analyses of transgenic mice (miR-29aTg mice) wild-type littermates subjected...

Abstract Motivation: Modern experimental biology is moving away from analyses of single elements to whole-organism measurements. Such measured time-course data contain a wealth information about the structure and dynamic pathway or network. The modeling whole systems formulated as reverse problem that requires well-suited mathematical model very efficient computational method identify parameters. Numerical integration for differential equations finding global parameter values are still two...

Prolonged glucocorticoid treatment is known to cause osteoporosis or aseptic necrosis. Secreted frizzled-related proteins 1 (SFRP1) and low-density lipoprotein-related protein 5 (LRP5), a Wnt antagonist coreceptor, have been found regulate skeletogenesis. Whereas recent studies reported that excess promotes bone loss, the biological role of SFRP1 LRP5 in regulating attenuation formation not fully understood. We showed supraphysiological level enhanced but expression primary mesenchymal cell...

The rapid growth of protein-protein interaction (PPI) data has led to the emergence PPI network analysis. Despite advances in high-throughput techniques, interactomes several model organisms are still far from complete. Therefore, it is desirable expand these with ortholog-based and other methods.Orthologous pairs 18 eukaryotic species were expanded merged experimental datasets. contributions interologs each evaluated. orthologous enable inference for various species. For example, more than...

Perturbation of Wnt signaling components reportedly regulates chondrocyte fate and joint disorders. The inhibitor Dkk-1 mediates remodeling various tissue types. We undertook this study to examine whether control expression prevents deterioration in osteoarthritic (OA) knees.Anterior cruciate ligament transection-and collagenase-induced OA rat knees was treated with end-capped phosphorothioate antisense oligonucleotide (Dkk-1-AS). Articular cartilage destruction, degradation markers, bone...

Background: This study investigated whether bone marrow–derived mesenchymal stem cell therapy has effectiveness in the enhancement of diabetic wound healing through tissue regeneration. Methods: The authors used a dorsal skin defect (6 × 5 cm) streptozotocin-induced diabetes rodent model. Forty male Wistar rats were divided into four groups: group I, nondiabetic (controls); II, controls receiving no cells; III, 1 107 cells per dose (subcutaneously administered eight areas surrounding margin)...