In this Feature Article, we focus on recent advances in our research molecular recognition and fluorescence sensing of phosphate anion derivatives biological importance. Because their significant roles systems, considerable efforts have been devoted to developing detection or determination systems. However, the these species under aqueous conditions using small-molecular chemosensors still remain as a challenging topic. We variety artificial receptors fluorescent for phosphoproteins...

We have developed a new fluorescent binuclear Zn(II) complex for the detection of neurofibrillary tangles (NFTs) hyperphosphorylated tau proteins, representative hallmark Alzheimer's disease (AD). The probe 1 incorporates BODIPY unit and two Zn(II)−2,2′-dipicolylamine (Dpa) complexes as binding site phosphorylated amino acid residues. Using fluorescence titration to evaluate sensing properties toward several peptide segments derived from protein, we found that binds preferentially peptides...

Highly syndiotactic poly(methyl methacrylate)s (PMMAs) with narrow molecular weight distribution (MWD) were prepared by polymerization of MMA in toluene at low temperatures, initiated tert-butyllithium (t-C4H9Li) combined trialkylaluminium compounds such as triethyl-, tributyl-, and trioctylaluminium (mole ratio Al/Li ≥ 3). Polymerization was the t-C4H9 anion proceeded a living manner. Several alkyl methacrylates also polymerized t-C4H9Li-(n-C4H9)3Al to give highly polymethacrylates MWD....

Human telomeric RNA has been identified as a key component of the telomere machinery. Recently, growing evidence suggests that forms G-quadruplex structures to play an important role in protection and regulation. In present studies, we developed 19F NMR spectroscopy method investigate vitro living cells. We demonstrated simplicity sensitivity approach can be used directly observe dimeric two-subunits stacked G-quadruplexes cells quantitatively characterize thermodynamic properties...

A cell growth inhibitory effect of drupanin and baccharin, ingredients propolis, was found in human cancer lines. These compounds induced apoptosis the cells characterized by morphological nucleosomal DNA fragmentation analysis. Their effects were less potent compared with that artepillin C, which is a known anticancer compound from propolis. Importantly, HL60 more sensitive to than Con A-stimulated peripheral blood lymphocytes, whereas potency C opposite drupanin.

Bee pollen, a honeybee product, is the feed for honeybees prepared themselves by pollens collecting from plants and has been consumed as perfect food in Europe, because it nutritionally well balanced. In this study, we aimed to investigate anti-inflammatory effect of bee pollen Cistus sp. Spanish origin method carrageenan-induced paw edema rats, mechanism action also elucidate components involved extracted with ethanol.The bulk, its water extract ethanol were administered orally rats. One...

To clarify the cis–trans isomeric effect on ultrafast DNA photo-cross-linking reaction of 3-cyanovinylcarbazole nucleoside (CNVK) in duplex, which gives a single photodimer reversed-phase HPLC chromatogram, kinetics photoisomerization CNVK double-stranded was evaluated. Since rate constant for cis to trans isomerization significantly larger than that isomerization, and thermodynamic stability isomer higher isomer, it strongly suggested is reactive species reaction. 1H–1H NOESY analysis...

In the living cell is where authors hope to elucidate structure of RNA by using their bispyrene-conjugated 2′-O-methyloligoribonucleotide (OMUpy2-I) fluorescence probe, detect hybridization with complementary RNA. The picture shows irradiated I) single-stranded OMUpy2-I, II) hybrid OMUpy2-I oligoRNA, and III) oligoDNA. Supporting information for this article available on WWW under http://www.wiley-vch.de/contents/jc_2002/2002/z19064_s.pdf or from author. Please note: publisher not...

Propolis, a honeybee product, has become popular as food and alternative medicine. Its constituents have been shown to exert pharmacological effects, such anticancer, antimicrobial, anti-inflammatory effects. The present study was performed investigate whether Brazilian green propolis exerts antihypertensive effects in spontaneously hypertensive rats (SHR) which are involved its extracted with ethanol subjected LH-20 column chromatography eluted ethanol. ethanol-eluted fractions at 10 mg/kg...

Brazilian propolis was extracted with water or various concentrations of ethanol and were administered orally to spontaneously hypertensive rats (SHR) the effects on blood pressure heart rate determined. Single oral administration 100 mg/kg extracts decreased in SHR. Significant decrease observed 25 70% ethanol. SHR given 5 ethanol, twice a day for 28 d compared control rats. While group increased by day, increase slower treated propolis. The hypotensive activity 25% more significant than...

We clearly demonstrated that photoreactive AS-ODNs having ^CNVK act as effective photo-regulators of constitutive GFP gene expression in living cells with only 10 s 366 nm irradiation.

3-Cyanovinylcarbazole modified D-threoninol ((CNV)D) was incorporated in oligodeoxyribonucleotide and tested for a photo-cross-linking reaction with complementary oligodeoxyribonucleotide. The photoreactivity 1.8- to 8-fold greater than that of 3-cyanovinylcarbazole deoxyribose ((CNV)K) previously reported. From the results melting analysis circular dichroism spectroscopy duplexes, relatively flexible structure (CNV)D compared (CNV)K might be advantageous [2 + 2] photocycloaddition between...

A tripodal quinone-cyanine dye having one donor and three acceptors, that is, quinone N-methylbenzothiazolium moieties, QCy(MeBT)3, was synthesized by simple Knoevenagel condensation between 2-hydroxybenzene-1,3,5-tricarbaldehyde N-methyl-2-methylbenzothiazolium iodide. The 700 nm (λex, 570 nm) 600 470 fluorescence emission of QCy(MeBT)3 significantly individually enhanced with the addition G-quadruplex (G4) DNA double-stranded (dsDNA), respectively. results docking simulations response...

ラットの膝関節腔内に種々の内因性発痛物質を投与することにより,関節疼痛モデルを作製し,Na-hyaluronate(SPH)の疼痛抑制作用について検討した.種々の発痛物質のうち,bradykinin(BK)とserotoninとでは投与量に依存した関節疼痛反応が明らかに認められたが,histamine,substance-P,acetylcholineでは顕著な疼痛反応は認められなかった.また,一般に病態関節では関節組織中のprosta-glandin量の増加や関節液中のhyaluronic acid(HA)の濃度及び分子量が低下していることが知られている.そこでBKによる疼痛モデルに対し,prostaglandin...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTViscoelastic properties of binary blends narrow molecular weight distribution polystyrenes. 2Hiroshi Watanabe, Takashi Sakamoto, and Tadao KotakaCite this: Macromolecules 1985, 18, 5, 1008–1015Publication Date (Print):May 1, 1985Publication History Published online1 May 2002Published inissue 1 1985https://pubs.acs.org/doi/10.1021/ma00147a034https://doi.org/10.1021/ma00147a034research-articleACS PublicationsRequest reuse permissionsArticle...

To visualize the guanine-quadruplex (G4) nucleic acids in cells or biological tissues, near-infrared (NIR) fluorescent probes that can respond specifically to G4 are required. Herein, an NIR fluorescence switch-on probe for imaging having higher selectivity and extremely large Stokes shift (ca. 220 nm) was successfully developed by modification of our original tripodal quinone-cyanine dye. The target binding-induced intramolecular stacking interaction might cause red shifting emission. here...

Although regioselective removal of 6-<i>O</i>-sulfate groups heparin has been undertaken by several researchers, complete 6-<i>O</i>-desulfation with little side reaction not attained successfully. In this work, a modified method certain silylating reagent,<i>N</i>-methyl-<i>N</i>-(trimethylsilyl)trifluoroacetamide, established to produce completely 6-<i>O</i>-desulfated few other chemical changes. The degrees were estimated means disaccharide analyses and/or ¹³C NMR spectra. the...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTEntanglements in linear polystyrenesHiroshi Watanabe, Takashi Sakamoto, and Tadao KotakaCite this: Macromolecules 1985, 18, 7, 1436–1442Publication Date (Print):July 1, 1985Publication History Published online1 May 2002Published inissue 1 July 1985https://pubs.acs.org/doi/10.1021/ma00149a014https://doi.org/10.1021/ma00149a014research-articleACS PublicationsRequest reuse permissionsArticle Views206Altmetric-Citations46LEARN ABOUT THESE...

Photo-induced artificial RNA editing was demonstrated using photo-reactive oligonucleotides containing 3-cyanovinylcarbazole nucleoside. This non-enzymatic and sequence-specific methodology will make a major contribution to the elucidation of functions including non-coding RNAs development drugs based on editing.

ADVERTISEMENT RETURN TO ISSUEPREVNoteIn Situ Measurement of Dielectrophoretic Mobility Single Polystyrene MicroparticlesHitoshi Watarai, Takashi Sakamoto, and Satoshi TsukaharaView Author Information Department Chemistry, Graduate School Science, Osaka University, Machikaneyama, Toyonaka, Osaka, 560 Japan Cite this: Langmuir 1997, 13, 8, 2417–2420Publication Date (Web):April 16, 1997Publication History Received31 October 1996Revised11 February 1997Published online16 April inissue 1...

Neuritic plaques are the key pathological feature of Alzheimer's disease, and amyloid β (Aβ) peptides major component these plaques. In this study, we demonstrated influence aluminum (Al) on Aβ peptide degradation by cathepsin D. Al did not directly affect D activity using small synthetic substrate. However, when were used as substrate, apparent inhibitory effect was observed. This disappeared treatment desferrioxamine. These results indicate that has potential to interact disrupt catabolism...

<b>Background: </b> Multifocal motor neuropathy (MMN) is often misdiagnosed as neuron disease, especially when overt evidence of conduction block (CB) lacking. Activity-dependent CB (ADCB), defined transient induced by brief exercise, has been recently found in MMN but not ALS. <b>Methods: To test the diagnostic utility ADCB for differentiating from ALS, authors recorded compound muscle action potentials (CMAPs) small hand muscles magnetically stimulating nerve roots before and after 1...

This study attempts to relate the 2,3,7,8-tetrachlorodibenzo-p-dioxin toxic equivalent (TEQ) level with certain responses including catalytic activities and expression of hepatic cytochrome P450 (CYP) 1A CYP1B in wild population Baikal seal (Pusa sibirica). We isolated full-length CYP1A1, 1A2, 1B1 cDNAs, which encode proteins 516, 512, 543 amino acids, respectively. Immunochemical analysis demonstrated that a cross-reactive protein polyclonal antibody against rat CYP1A1 or CYP1B1 was...