A5091907831- Viral gastroenteritis research and epidemiology
- Mesenchymal stem cell research
- Child Nutrition and Water Access
- Tissue Engineering and Regenerative Medicine
- Clinical Nutrition and Gastroenterology
- Dental Implant Techniques and Outcomes
- Pelvic floor disorders treatments
- Infant Nutrition and Health
- Cancer Cells and Metastasis
- Probiotics and Fermented Foods
- Child Nutrition and Feeding Issues
- Bone Tissue Engineering Materials
- Pluripotent Stem Cells Research
- Hernia repair and management
- Stroke Rehabilitation and Recovery
- Bone health and osteoporosis research
- Cellular Mechanics and Interactions
- Infant Health and Development
- Periodontal Regeneration and Treatments
- Urological Disorders and Treatments
- Neurogenesis and neuroplasticity mechanisms
- Adipose Tissue and Metabolism
- Wound Healing and Treatments
- Adolescent and Pediatric Healthcare
- Adipokines, Inflammation, and Metabolic Diseases
Tampere University
1989-2023
Tampere University Hospital
2012-2023
FIT Biotech (Finland)
2012
Helsinki University Hospital
2001
Satakunta Central Hospital
1989-1997
Adipose tissue is an attractive and abundant source of multipotent stem cells. Human adipose cells (ASCs) have shown to therapeutic relevancy in diverse clinical applications. Nevertheless, expansion ASCs often necessary before performing studies. Standard vitro cell-culture techniques use animal-derived reagents that should be avoided because safety issues. Therefore, xeno- serum-free (XF/SF) are highly desirable for enhancing the quality transplanted ASCs. In current study, animal...
Successful management of gastroenteritis in children relies chiefly on the maintenance or restoration adequate hydration and electrolyte balance together with an nutritional intake. Earlier debate centred composition oral rehydration solution (ORS), which was considered optimal (1). In 1992 European Society Paediatric Gastroenterology Nutrition (ESPGAN) Working Group Oral Rehydration Therapy (ORT) published guidelines for ORS use Europe. A hypoosmolar glucose-based 60mmol/L sodium...
The main aim in managing gastroenteritis children is the correction of dehydration and maintenance hydration using an optimal oral rehydration solution (ORS). In 1992 European Society Paediatric Gastroenterology Nutrition (ESPGAN) working group on therapy (ORT) published guidelines for composition ORS Europe (1). second management these nutritional repair. positive effect continuing breast-feeding during treatment with well established (2). However, debate has continued non-breast-fed...
The effects of early home management acute diarrhea followed by rapid in-hospital realimentation on intestinal permeability were studied in 41 children aged 3-25 months with gastroenteritis (73% rotavirus). After oral rehydration, a 100 ml load containing 4 g lactulose and 0.8 mannitol was administered, an aliquot urine excreted the subsequent 5 h analyzed gas-liquid chromatography. mean lactulose/mannitol recovery ratio significantly higher than 28 nondiarrheal controls, which due to...
Bone morphogenetic protein-2 (BMP-2) is a growth factor used to stimulate bone regeneration in clinical applications. However, there are contradicting reports on the functionality of BMP-2 human adipose stem cells (hASCs), which frequently tissue engineering. In this study, we analyzed effects SMAD1/5 signaling, proliferation, and differentiation hASCs. Our results indicated that induced dose-dependent (25-100 ng/ml) activation SMAD signaling. Furthermore, cell proliferation analysis...
Despite the good performance of silicate bioactive glasses in bone regeneration, there is considerable potential to enhance their properties by chemical modifications. In this study, S53P4-based borosilicate were synthesized and dissolution profile was studied simulated body fluid assessing pH change, ion release conversion hydroxyapatite. The viability, proliferation, attachment, osteogenesis endothelial marker expression human adipose stem cells (hASCs) evaluated upon direct culture on...
Background/Objective: Stroke is a leading global cause of adult disability. As the population ages as well suffers co-morbidities, it expected that stroke burden will increase further. There are no established safe and effective restorative treatments to facilitate good functional outcome in patients. Cell-based therapies, which have wide therapeutic window, might benefit large percentage patients, especially if combined with different strategies. In this study, we tested whether effect...
Adipose tissue is an attractive stem cell source for soft and bone engineering applications therapies. The adipose-derived stromal/stem cells (ASCs) have a multilineage differentiation capacity that regulated through extracellular signals. cellular events related to adhesion cytoskeleton been suggested as central regulators of fate decision. However, the detailed knowledge these molecular mechanisms in human ASCs remains limited. This study examined significance focal kinase (FAK),...
Anal incontinence is a devastating condition that significantly reduces the quality of life. Our aim was to evaluate effect human adipose stem cell (hASC) injections in rat model for anal sphincter injury, which main cause humans. Furthermore, we tested if efficacy hASCs could be improved by combining them with polyacrylamide hydrogel carrier, Bulkamid. Human ASCs derived from female donor were culture expanded DMEM/F12 supplemented platelet lysate. Female virgin Sprague-Dawley rats...
Abstract Background Adipose stromal/stem cells (ASCs) are promising candidates for future clinical applications. ASCs have regenerative capacity, low immunogenicity, and immunomodulatory ability. The success of cell-based therapies depends on the appropriate selection donors. Several factors, including age, sex, body mass index (BMI), may influence ASC characteristics. Our aim was to investigate effect acquired weight characteristics under same genetic background using derived from...
For adipose stromal/stem cell (ASCs)-based immunomodulatory therapies, it is important to study how donor characteristics, such as obesity and type 2 diabetes (T2D), influence ASCs efficacy. Here, were obtained from groups, donors with T2D (dASCs) or nondiabetic normal-weight (ndASCs), then cultured anti-CD3/CD28-stimulated allogeneic CD4 T cells. studied for the expression of immunomodulators CD54, CD274, indoleamine 2, 3 dioxygenase 1 (IDO) in inflammatory conditions. cells alone...
The surgical reconstruction of functional neovagina is challenging and susceptible to complications. Therefore, developing tissue engineering-based treatment methods for vaginal defects important. Our aim was develop test a novel supercritical carbon dioxide foamed poly- l -lactide-co-ɛ-caprolactone (scPLCL) scaffold reconstruction. scaffolds were manufactured characterized porosity (65 ± 4%), pore size (350 150 µm) elastic modulus (2.8 0.4 MPa). Vaginal epithelial (EC) stromal cells (SC)...
Stroke is a devastating neurological disorder and one of the leading causes mortality disability. To understand cellular molecular mechanisms stroke to develop novel therapeutic approaches, two different in vitro human cell-based models were established using oxygen-glucose deprivation (OGD) conditions. In addition, effect adipose stem cells (ASCs) on OGD-induced injury was studied. present study, SH-SY5Y neuroblastoma induced pluripotent (hiPSCs) differentiated into neurons, cultured under...
The effects of bioactive glass S53P4 or beta-tricalcium phosphate; and bone morphogenetic proteins protein-2, protein-7, protein-2 + 7 on osteogenic differentiation human adipose stem cells were compared in control medium, protein-supplemented medium to assess suitability for tissue engineering. Cell amount was evaluated with qDNA measurements; using marker gene expression, alkaline phosphate activity, angiogenic potential measured by vascular endothelial growth factor expression. As...
Microglia are involved in the post-stroke immunomodulation of brain plasticity, repair, and reorganization. Here, we evaluated whether adipose-tissue-derived mesenchymal stem cells (ADMSCs) and/or rehabilitation improve behavioral recovery by modulating long-term perilesional inflammation creating a recovery-permissive environment rat model ischemic stroke. Methods: A two-way mixed lymphocyte reaction was used to assess immunomodulatory capacity ADMSCs vitro. Two or 7 days after permanent...
<h3>AIM</h3> To evaluate the effect of maternal diabetes on concentrations free and bound leptin at birth during postnatal adaptation. <h3>METHODS</h3> Total, bound, percentage were measured in cord plasma 3 days age 13 term infants mothers with gestational mellitus (GDM) healthy mothers. Gestational was 40.2 (1.4) weeks, weight 3693 (549) g (means (SD)). <h3>RESULTS</h3> At birth, GDM had significantly higher total, a (all p < 0.05). In all infants, these lower than 0.003), differences...
Summary The effects of early home management acute diarrhea followed by rapid in‐hospital realimentation on intestinal permeability were studied in 41 children aged 3–25 months with gastroenteritis (73% rotavirus). After oral rehydration, a 100 ml load containing 4 g lactulose and 0.8 mannitol was administered, an aliquot urine excreted the subsequent 5 h analyzed gas‐liquid chromatography. mean lactulose/mannitol recovery ratio significantly higher than 28 nondiarrheal controls, which due...