A5082473660- Multiple Sclerosis Research Studies
- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neural Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Full-Duplex Wireless Communications
- Pluripotent Stem Cells Research
- Polyomavirus and related diseases
- Advanced Neuroimaging Techniques and Applications
- MicroRNA in disease regulation
- 3D Printing in Biomedical Research
- Systemic Lupus Erythematosus Research
- Antenna Design and Analysis
- Circular RNAs in diseases
- Cytokine Signaling Pathways and Interactions
- Cell Image Analysis Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Research and Treatments
- Fetal and Pediatric Neurological Disorders
- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Tryptophan and brain disorders
- Neuroscience and Neuropharmacology Research
- Salivary Gland Disorders and Functions
- Glioma Diagnosis and Treatment
- Advanced MRI Techniques and Applications
Tampere University
2015-2025
Tampere University Hospital
2011-2022
Abstract Astrocyte reactivation has been discovered to be an important contributor several neurological diseases. In vitro models involving human astrocytes have the potential reveal disease-specific mechanisms of these cells and advance research on neuropathological conditions. Here, we induced a reactive phenotype in pluripotent stem cell (hiPSC)-derived studied inflammatory natures effects neurons. Astrocytes responded interleukin-1β (IL-1β) tumor necrosis factor-α (TNF-α) treatment with...
Background: In multiple sclerosis (MS), microRNA (miRNA) dysregulation is mostly reported in different immune cells, but less information available on circulating miRNAs that exert strong biomarker potential due to their exceptional stability body fluids. Objective: The aim of this study was profile expression primary progressive (PPMS) and secondary (SPMS) assess association with neurological worsening. Methods: expressions 84 were profiled serum 83 subjects (62 MS 21 controls) using...
Background Biomarkers that could be used in early diagnosis of multiple sclerosis (MS), segregation disease subtypes, and discrimination the aggressive course from benign one are urgently needed. Objective The aim this study was to investigate specificity circulating microRNAs: miR-191-5p, miR-128-3p, miR-24-3p, miR-376c-3p MS evaluate their association with activity disability progression. Methods expressions miRNAs were studied serum 100 subjects (53 relapsing-remitting (RRMS), 20 primary...
Background: Microglia and astrocytes have been implicated as central mediators of neuroinflammatory processes in several neurodegenerative diseases. However, their intricate crosstalk contributions to pathogenesis remain elusive, highlighting the need for innovative vitro approaches investigating glial interactions neuroinflammation. The aim this study was develop advanced human-based coculture models explore inflammatory roles microglia . Methods: We utilized human induced pluripotent stem...
Abstract Objectives Diffusion tensor imaging (DTI) is sensitive technique to detect widespread changes in water diffusivity the normal‐appearing white matter (NAWM) that appears unaffected conventional magnetic resonance imaging. We aimed investigate prognostic value and stability of DTI indices NAWM brain an assessment disability progression patients with a relapsing‐onset multiple sclerosis (MS). Methods Forty‐six MS were studied for (fractional anisotropy (FA), mean (MD), radial (RD),...
Abstract Axonal dysfunction and degeneration are important pathological features of central nervous system (CNS) diseases traumas, such as Alzheimer's disease, traumatic brain injury, ischemic stroke spinal cord injury. Engineered microfluidic chips combined with human pluripotent stem cell (hPSC)‐derived neurons provide valuable tools for targeted in vitro research on axons to improve understanding disease mechanisms enhance drug development. Here, a polydimethylsiloxane (PDMS) chip...
Background The risk of progressive multifocal leukoencephalopathy (PML) caused by the JC virus (JCV) is increased in patients with multiple sclerosis receiving biological therapies. Objectives To determine seroprevalence anti-JCV antibodies Finnish (MS) and clinically isolated syndrome to assess clinical factors for JCV seropositivity. Methods was analyzed 503 using a second-generation two-step ELISA. Sixty-seven underwent longitudinal serological evaluation over 4.5 years. Results overall...
Background and Objective . The role of adipokines in regulation immune responses has been recognized, but very little is known about their impact on multiple sclerosis (MS). In this study, we analysed whether the major are differentially expressed plasma patients with different MS subtypes clinically isolated syndrome (CIS) explored association disease characteristics. Methods levels adiponectin, adipsin, leptin, resistin 80 CIS were followed up annually over two years. data obtained...
Stroke is a devastating neurological disorder and one of the leading causes mortality disability. To understand cellular molecular mechanisms stroke to develop novel therapeutic approaches, two different in vitro human cell-based models were established using oxygen-glucose deprivation (OGD) conditions. In addition, effect adipose stem cells (ASCs) on OGD-induced injury was studied. present study, SH-SY5Y neuroblastoma induced pluripotent (hiPSCs) differentiated into neurons, cultured under...
Human pluripotent stem cell (hPSC)-derived neural cultures have attracted interest for modeling epilepsy and seizure-like activity in vitro. Clinical experimental evidence shown that the multifunctional inflammatory cytokine interleukin (IL)-6 plays a significant role epilepsy. However, of IL-6 neuronal networks remains unclear. In this study, we modelled hPSC-derived cortical neurons using kainic acid (KA) explored effects its counterpart, hyper-IL-6 (H-IL-6), fusion protein consisting...
The possibilities of human pluripotent stem cell-derived neural cells from the basic research tool to a treatment option in regenerative medicine have been well recognized. These also offer an interesting for vitro models neuronal networks be used drug screening and neurotoxicological studies patient/disease specific models. Here, as aiming develop reductionistic network model, we tested whether embryonic cell (hESC)-derived could cultured cerebrospinal fluid (CSF) order better mimic vivo...
The aim of this study was to evaluate diffusion tensor imaging (DTI) indices in the corpus callosum and pyramidal tract normal-appearing white matter (NAWM) caudate nucleus thalamus deep grey (NADGM) all MS subtypes clinically isolated syndrome (CIS). Furthermore, it determined whether these metrics are associated with clinical measures serum levels candidate immune biomarkers. Apparent coefficients (ADC) values were significantly higher than controls six studied NAWM regions SPMS, 4/6 RRMS...
This paper addresses two subtypes of multiple sclerosis (MS), primary progressive (PPMS) and relapsing-remitting (RRMS). The separation PPMS RRMS is challenging in certain cases.To quantitatively determine MS using texture analysis (TA) diffusion tensor imaging (DTI).T1-weighted (T1W) magnetic resonance (MRI) DTI the left right brain hemispheres 17 patients with 19 were studied. Areas caudate nucleus thalamus investigated as normal appearing gray matter (NAGM), areas cerebral peduncle...
Multiple sclerosis (MS) is a complex autoimmune disease of the central nervous system where main pathogenetic events include demyelination and axonal degeneration. Here, we generated human induced pluripotent stem cell (hiPSC) line from peripheral blood mononuclear cells an MS patient utilizing Sendai virus reprogramming. The produced hiPSC expressed pluripotency markers, differentiated into three germ layers, showed normal karyotype was free vectors, transgenes mycoplasma. Established...
Abstract BACKGROUND Diffuse astrocytomas, especially glioblastomas (GBs), are aggressive brain tumors with a poor prognosis despite the efforts to improve their treatment. In diffuse myeloid immune cells, notably monocyte-derived macrophages and brain-resident microglia pivotal in immunoregulation of tumor microenvironment (TME). GBs, characterized by higher macrophage frequencies, often exhibit hypoxia TME. this work, we investigated how affects regulation cells. MATERIAL AND METHODS We...
Abstract Aberrant and sustained activation of microglia is implicated in the progression severity multiple sclerosis (MS). However, whether intrinsic alterations microglial function impact pathogenesis this disease remains unclear. We conducted transcriptomic functional analyses microglia-like cells (iMGLs) differentiated from induced pluripotent stem (iPSCs) patients with MS (pwMS) to answer question. generated iPSCs six pwMS showing increased activity via translocator protein (TSPO)-PET...